Classification of Bacteroides nodosus by agglutination tests

Claxton, P. D.; Ribeiro, Lima; Egerton, J. R.

doi:10.1111/j.1751-0813.1983.tb02834.x

Cited by 129 publications

(80 citation statements)

References 12 publications

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“…RESULTS Genetic constructions. The B. nodosus fimbrial subunit gene used in this study was derived from strain VCS1001, which is the designated prototype of serogroup A (8). The gene is located within a 5.5-kilobase HindlIl genomic DNA fragment and is expressed in E. coli from an associated promoter within the cloned sequence (1).…”

Section: )mentioning

confidence: 99%

Morphogenetic expression of Bacteroides nodosus fimbriae in Pseudomonas aeruginosa

Mattick¹,

Bills²,

Anderson³

et al. 1987

J Bacteriol

105

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Type 4 fimbriae are found in a range of pathogenic bacteria, including Bacteroides nodosus, Moraxella bovis, Neisseria gonorrhoeae, and Pseudomonas aeruginosa. The structural subunits of these fimbriae all contain a highly conserved hydrophobic amino-terminal sequence preceding a variable hydrophilic carboxy-terminal region. We show here that recombinant P. aeruginosa cells containing the B. nodosus fimbrial subunit gene under-the control of a strong promoter (PL, from bacteriophage A) produced large amounts of fimbriae that were structurally and antigenically indistinguishable from those produced by B. nodosus. This was demonstrated by fimbrial isolation and purification, electrophoretic and Western transfer analyses, and immunogold labeling and electron microscopy. These results suggest that type 4 fimbriated bacteria use a common mechanism for fimbrial assembly and that the structural subunits are interchangeable, thereby providing a basis for the development of multivalent vaccines.Bacteroides nodosus is the essential causative agent of ovine footrot (4, 11). This anaerobe contains numerous surface filaments, about 6 nm in diameter and ranging up to several micrometers in length (14, 46, 50), termed fimbriae (or common pili), which play a central role in both pathogenesis and immunity (for a recent review, see reference 29).Fimbriae have adherent functions and appear to be a mechanism for the colonization of epithelial tissues in eucaryotic hosts. The properties of B. nodosus fimbriae (14) suggest that they belong in the category of type 4, as proposed by Ottow (38), citing Pseudomonas aeruginosa (6) as a prototype. Fimbriae of this type have a polar location on the cell and appear to be involved in surface translocation by a phenomenon known as twitching motility (21). The same characteristics are also observed in the fimbriae found in a broad range of gram-negative species classified within the genera Acinetobacter, Alteromonas, Bacteroides, Eikenella, Moraxella, Neisseria, and Pseudomonas, among others (6,17,20,21).This grouping is supported by recent protein and DNA sequence analyses of the structural subunits of the fimbriae of B. nodosus (12, 31), Moraxella nonliquefaciens (16), Moraxella bovis (28), Neisseria gonorrhoeae (22,33,44), Neisseria meningitidis (22, 36), and P. aeruginosa (42). These subunits, which range in size from about 145 to 160 amino acids among different species and serotypes, all share the distinctive feature of an unusual modified amino acid, methylphenylalanine (MeF), as the first residue in the mature protein, as well as a striking degree of sequence conservation throughout the amino-terminal region. This region is highly hydrophobic and exhibits at least 90% homology with the following 32-amino-acid consensus sequence:MeF T L I E L M I V

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Section: )mentioning

confidence: 99%

Morphogenetic expression of Bacteroides nodosus fimbriae in Pseudomonas aeruginosa

Mattick¹,

Bills²,

Anderson³

et al. 1987

J Bacteriol

105

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“…The genes are grouped in several gene clusters. The major fimbrial subunit protein (FimA), encoded by fimA, is strain-specific and is used to divide the species into different serological groups (15). Variations of fimA amongst different serogroups have been confirmed by PCR-SSCP analyses (114).…”

Section: Dichelobacter Nodosusmentioning

confidence: 98%

Footrot in Clawed and Hoofed Animals: Symptoms, Causes and Treatments

Petrov¹,

Dicks²

2013

Biotechnology & Biotechnological Equipment

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“…The level of amino acid identity was 40.5% to the pectin lyase regulator RdgA of Erwinia carotouora (Liu et al, 1995) (Stevens et al, 1993). The gepB gene encodes a protein with 64-9 Yo amino acid similarity over 188 amino acids to an unidentified ORF in the cob gene cluster from Pseudornonas denitrificans (Crouzet et al, 1991), while the gepC gene shows no significant similarity to any genes in the databases.…”

Section: Identification Of An Integrase Gene To the Right Of Wap Regimentioning

confidence: 99%

“…It is of interest that the Salmonella virulence plasmid also contains a gene required for cobalamin transport (Rioux et al, 1990), as we have found similarity between the gene gepB and a gene from the cob gene cluster of Pseudomonas denitrificans (Crouzet et al, 1991). In addition, the Salmonella virulence plasmid contains a gene, rsd, with similarity to the lambda family of integrases (Krause & Guiney, 1991).…”

Section: A Killer System In D Nodosus?mentioning

confidence: 99%

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Analysis of sequences flanking the vap regions of Dichelobacter nodosus: evidence for multiple integration events, a killer system, and a new genetic element

et al. 1997

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Dichelobacter nodosus is the causative agent of ovine footrot. The wap regions of the D. nodosus genome may have arisen by the integration of a genetic element and may have a role in virulence. The virulent D. nodosus strain A198 has multiple copies of the wap regions. In the present study, sequences to the left and right of wap regions 1,2 and 3 of strain A198 were analysed by Southern blotting and DNA sequencing. The results suggest that wap regions 1 and 2 arose by independent integration events into different tRNA genes. The discovery of a second integrase gene (intB), a gene with similarity to bacteriophage repressor proteins (regA), and a gene similar to an ORF from a conjugative transposon (gepA), suggests that a second genetic element either a bacteriophage or a conjugative transposon, is integrated next to wap region 3 in the D. nodosus genome. The arrangement of inti3 and the wap regions in three other virulent strains and one benign strain was determined using using Southern blotting and PCR. One strain, H1215, contained wapF and not wapE, and thus resembles wap region 3, suggesting that wap region 3 also may have arisen by an independent integration event. In all strains, a copy of intS was found next to the wap regions. The wap regions contain two genes, wapA and toxA, with similarity to the hig genes of the killer plasmid Rtsl. Evidence is presented that wapA and toxA have a similar function in D. nodosus.

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Classification of Bacteroides nodosus by agglutination tests

Cited by 129 publications

References 12 publications

Morphogenetic expression of Bacteroides nodosus fimbriae in Pseudomonas aeruginosa

Morphogenetic expression of Bacteroides nodosus fimbriae in Pseudomonas aeruginosa

Footrot in Clawed and Hoofed Animals: Symptoms, Causes and Treatments

Analysis of sequences flanking the vap regions of Dichelobacter nodosus: evidence for multiple integration events, a killer system, and a new genetic element

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