Classification of anti-FcϵRI and anti-IgE autoantibodies in chronic idiopathic urticaria and correlation with disease severity

Sabroe, R.A.; Fiebiger, Edda; Francis, David M.; Maurer, Dieter; Seed, Paul; Grattan, Clive; Black, A. Kobza; Stingl, Georg; Greaves, Malcolm W.; Barr, Robert M.

doi:10.1067/mai.2002.126782

Cited by 249 publications

(252 citation statements)

References 19 publications

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“…Clinical and demowheal-and-flare reactions much more frequently than ASST, and observed an increase of thrombin generation in plasma of patients with CSU, suggesting a mechanism of mast cells degranulation different from autoantibody-mediated activation (8). Histamine is the most important mediator in CU which is released upon stimulation by both circulating basophils and tissue-infiltrating mastocytes (1,3). Functional assay of histamine release (HR) from healthy non atopic donors' basophils incubated with CU patients' serum has previously been shown to have a good sensitivity and specificity for endogenous histamine-releasing factors presence, either antibodies or not (9).…”

Section: Study Cohortmentioning

confidence: 99%

“…Chronic spontaneous urticaria (CSU) refers more specifically to those form of CU not caused by vascular inflammation (urticarial vasculitis) or triggered by an identifiable external factor, including physical stimuli, and inherited or acquired inflammatory diseases. Approximately 30-50% of cases of CSU are thought to have an autoimmune etiology, primarily correlated to the presence of IgG autoantibodies directed against FcεRIα receptor or less commonly IgE, that leads to mast cells degranulation (1)(2)(3)(4)(5). The demonstration of these autoantibodies is not usually part of common clinical practice.…”

Section: Introductionmentioning

confidence: 99%

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Histamine release positive test associates with disease remission in chronic spontaneous urticaria: a proof-of-concept study

Berti

Yacoub

Skov

et al. 2017

Eur Ann Allergy Clin Immunol

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Section: Study Cohortmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Histamine release positive test associates with disease remission in chronic spontaneous urticaria: a proof-of-concept study

Berti

Yacoub

Skov

et al. 2017

Eur Ann Allergy Clin Immunol

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“…Bu çalışma sonucunda ayrıca OSDT pozitifliği olan hastaların Konstantinou ve arkadaşlarının 25 belirttiği gibi tiroid patolojileri açısından araştırılması gerektiği ifade edilmiştir 22 . Son yayınlarda KÜ tanılı olguların %23-40 kadarında yüksek afiniteli IgE reseptörüne karşı IgG yapısında otoantikorlar saptanmış 26,27 olup, ilave olarak %5-9 kadarında da fonksiyonel anti IgE antikorları saptanmıştır 27,28 . Bu durum kronik otoimmun ürtiker (KOÜ) olarak ifade edilmektedir.…”

Section: Discussionunclassified

Kronik ürtikerli olgularımızın etyolojik değerlendirmesi

Ayyıldız¹,

Sözmen²,

Fırıncı³

et al. 2014

TURKDERM

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ÖzetAmaç: Çocukluk çağında sınırlı veri nedeniyle kronik ürtiker (KÜ) etyolojisi tam olarak anlaşılamamıştır. Bu çalışmada kronik ürtiker tanılı çocuklarda muhtemel etyolojik nedenlerin değerlendirilmesini amaçladık. Gereç ve Yöntem: Ocak 2011 ile Temmuz 2012 tarihleri arasında KÜ tanısı ile kliniğimizde izlenen 18 yaş altı 67 hasta retrospektif olarak analiz edildi. Kronik ürtiker tanılı hastalarda otoimmun hastalıklar, maliniteler, infeksiyonlar gibi etyolojik faktörler araştırıldı. Veriler ortalama±standart sapma (SD) ve yüzde (%) olarak belirtildi. İki grup karşılaştırması Mann-Whitney U metodu kullanılarak yapıldı. P<0,05 olması istatiksel açıdan anlamlı kabul edildi. Bulgular: Kronik ürtiker (KÜ) tanısı olan 67 hasta çalışmaya alındı. Erkek/kız oranı:22/45, ortalama semptom başlangıç yaşı 10,2±4,6 (1-17) yıl idi. Hastaların %7'sinde ANA titresi >1/100 saptanmış olup bu hastaların hiçbirinde romatolojik hastalığa ait klinik özellik belirtilmediği öğrenildi. Hastaların %13,7'sinde tiroid otoantikor pozitifliği olup, hepsine tiroidit tanısı konulduğu ve L-Tiroxin tedavisi başlandığı görüldü. Otolog serum deri testi hastaların %48,2 (n=28)'sinde pozitif olarak saptandı. Sonuç: Çocuklarda infeksiyonlar ve besinler önemli birer KÜ nedeni gibi görünmemektedir. Erişkinlerde olduğu gibi çocuklarda da KÜ etyolojisinde otoreaktif süreçler önemli bir yer tutmaktadır. Bu sonuçlar daha önce KÜ tanılı Türk çocuklarda yapılan çalışmalar ile benzer özelliktedir. (Türk derm 2014; 48: 13-6) Anah tar Ke li me ler: Kronik ürtiker, otolog serum testi, antinükleer antikor, tiroidit Sum maryBackground and Design: The etiology of chronic urticaria (CU) in childhood remains incompletely understood because of limited data. In this study, we aimed to examine possible etiologies of CU in children. Materials and Methods:We retrospectively analyzed the medical records of 67 children who attended our clinic and were diagnosed with CU between January 2001 and July 2012. We investigated the etiological factors contributing to chronic urticaria, such as autoimmune disorders, malignancies and infections. Data were presented as mean±standard deviation (SD) and percentage (%). The comparisons between two groups were conducted using the Mann-Whitney U test. A p value of less than 0.05 was considered statistically significant. Results: Sixty-seven children who met the criteria for CU were included in the study. Male/female ratio was 0.5 and the mean age at onset of symptoms was 10.2±4.6 (range: 1-17) years. In 7% of patients, ANA titers were over 1/100. None of the patients had clinical features of a rheumatic disease, 13.7% of the subjects were positive for thyroid autoantibody. All of them were diagnosed with thyroiditis and received L-thyroxine treatment. Autologous serum skin test was positive in 42.8% of the patients (n=28). Conclusion: In children, infections and foods did not appear to be important causative factors for CU. As in adults, the etiology of CU in children is mainly related to autoreactive processes. This result is similar to ...

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“…However, CBM␣8 did not block the membrane-proximal epitope on Fc⑀RI␣, as seen by binding of the control mAb 5H5F8. In CU cases, the presence of anti-Fc⑀RI␣ Abs in serum has been frequently reported (53)(54)(55), but the epitopes of these Abs have not been elucidated. We have confirmed that CBM␣8 induces degranulation in vitro by measuring the release of the preformed granular enzyme ␤-hexosaminidase from RBL-2H3 cells expressing functional human Fc⑀RI␣ and by measuring histamine release from purified human basophils.…”

Section: Discussionmentioning

confidence: 99%

A High-Affinity Natural Autoantibody from Human Cord Blood Defines a Physiologically Relevant Epitope on the FcεRIα

Bobrzynski

Fux

Vogel

et al. 2005

The Journal of Immunology

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Natural Abs represent the indigenous immune repertoire and are thus present at birth and persist throughout life. Previously, human autoantibodies to the α domain of the high-affinity IgE receptor (FcεRIα) have been isolated from Ab libraries derived from normal donors and patients with chronic urticaria. To investigate whether these anti-FcεRIα Abs are present in the germline repertoire, we constructed a phage Fab display library from human cord blood, which represents the naive immune repertoire before exposure to exogenous Ags. All isolated clones specific to the FcεRIα had the same sequence. This single IgM Ab, named CBMα8, was strictly in germline configuration and had high affinity and functional in vitro anaphylactogenic activity. Inhibition experiments indicated an overlapping epitope on the FcεRIα recognized by both CBMα8 and the previously isolated anti-FcεRIα Abs from autoimmune and healthy donors. This common epitope on FcεRIα coincides with the binding site for IgE. Affinity measurements demonstrated the presence of Abs showing CBMα8-like specificity, but with a significantly lower affinity in i.v. Ig, a therapeutic multidonor IgG preparation. We propose a hypothesis of escape mutants, whereby the resulting lower affinity IgG anti-FcεRIα Abs are rendered less likely to compete with IgE for binding to FcεRIα.

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Classification of anti-FcϵRI and anti-IgE autoantibodies in chronic idiopathic urticaria and correlation with disease severity

Cited by 249 publications

References 19 publications

Histamine release positive test associates with disease remission in chronic spontaneous urticaria: a proof-of-concept study

Histamine release positive test associates with disease remission in chronic spontaneous urticaria: a proof-of-concept study

Kronik ürtikerli olgularımızın etyolojik değerlendirmesi

A High-Affinity Natural Autoantibody from Human Cord Blood Defines a Physiologically Relevant Epitope on the FcεRIα

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