Classification and biomarker identification of prostate tissue from TRAMP mice with hyperpolarized 13C-SIRA

Fine optimization of a dissolution dynamic nuclear polarization experimental setting for 13C NMR of metabolic samples

“…However, several recent studies highlighted the potential of d-DNP for analyzing complex metabolic mixtures by 13 C NMR. Recent studies demonstrated the relevance of d-DNP for fluxomic studies by quantifying the 13 C isotopic patterns to understand the metabolic activity of cancer cell extract incubated with 13 C-enriched glucose (Frahm et al, 2021;Lerche et al, 2018;Frahm et al, 2020). Parallel investigations also focused on the development of d-DNP methods to analyze metabolic samples at natural 13 C abundance, taking advantage of 1 H → 13 C cross-polarization (CP) in the solid state to reach high 13 C polarization levels in a short time (Batel et al, 2014;Bornet et al, 2013).…”

NMR methods for the analysis of mixtures

“…In many systems, the dissolution, transfer and stabilisation times add up to more than 10 s. 87 This is why most applications of D-DNP rely on the hyperpolarisation of quaternary carbons, which have relaxation time constants that can be larger than 20 s. Several groups have also reported instrumentation developments that make is possible to reduce the delay between dissolution and detection to less than 5 seconds. [88][89][90][91] With such devices, a broader range of chemical sites can be observed. D-DNP is used extensively for kinetic studies based on the injection of hyperpolarised compounds in a mixture containing other reactants and/or catalysts, or in cell cultures.…”

“…D-DNP is also investigated as a method to increase sensitivity in NMR metabolomics. 91,[102][103][104][105] Lerche and co-workers have used D-DNP for sensitivity enhanced stable-isotope resolved analysis (SIRA). 91,103 In this approach, a biological system is fed with 13 C-labelled sugars for a period of time, before undergoing metabolic quenching and extraction.…”

“…91,[102][103][104][105] Lerche and co-workers have used D-DNP for sensitivity enhanced stable-isotope resolved analysis (SIRA). 91,103 In this approach, a biological system is fed with 13 C-labelled sugars for a period of time, before undergoing metabolic quenching and extraction. DNPenhanced 13 C-NMR spectra of the extracts are then quantitatively analysed to classify sample types and identify potential biomarkers.…”

Fine optimization of a dissolution-DNP experimental setting for <sup>13</sup>C NMR of metabolic samples

“…However, several recent studies highlighted the potential of d-DNP for analyzing complex metabolic mixtures by 13 C NMR. Lerche et al demonstrated the relevance of d-DNP for fluxomic studies by quantifying the 13 C isotopic patterns to understand the metabolic activity of cancer cell extract incubated with 13 C-enriched glucose (Frahm et al, 2021;Lerche 65 et al, 2018;Frahm et al, 2020). Recent investigations also focused on the development of d-DNP methods to analyze metabolic samples at natural 13 C abundance, taking advantage of 1 H → 13 C cross polarization (CP) in the solid-state to reach high 13 C polarization levels in a short time (Batel et al, 2014;Bornet et al, 2013).…”