Classical swine fever virus: the past, present and future

Ganges, Llilianne; Crooke, Helen; Bohórquez, José Alejandro; Postel, Alexander; Sakoda, Yoshihiro; Becher, Paul; Ruggli, Nicolas

doi:10.1016/j.virusres.2020.198151

Cited by 114 publications

(148 citation statements)

References 334 publications

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“…The Pestivirus genus includes economically important members such as bovine viral diarrhea virus (BVDV) and classical swine fever virus (CSFV) [47].…”

Section: Groupmentioning

confidence: 99%

Potential for Protein Kinase Pharmacological Regulation in Flaviviridae Infections

Blázquez

Saiz

2020

IJMS

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Protein kinases (PKs) are enzymes that catalyze the transfer of the terminal phosphate group from ATP to a protein acceptor, mainly to serine, threonine, and tyrosine residues. PK catalyzed phosphorylation is critical to the regulation of cellular signaling pathways that affect crucial cell processes, such as growth, differentiation, and metabolism. PKs represent attractive targets for drugs against a wide spectrum of diseases, including viral infections. Two different approaches are being applied in the search for antivirals: compounds directed against viral targets (direct-acting antivirals, DAAs), or against cellular components essential for the viral life cycle (host-directed antivirals, HDAs). One of the main drawbacks of DAAs is the rapid emergence of drug-resistant viruses. In contrast, HDAs present a higher barrier to resistance development. This work reviews the use of chemicals that target cellular PKs as HDAs against virus of the Flaviviridae family (Flavivirus and Hepacivirus), thus being potentially valuable therapeutic targets in the control of these pathogens.

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“…The Pestivirus genus includes economically important members such as bovine viral diarrhea virus (BVDV) and classical swine fever virus (CSFV) [47].…”

Section: Groupmentioning

confidence: 99%

Potential for Protein Kinase Pharmacological Regulation in Flaviviridae Infections

Blázquez

Saiz

2020

IJMS

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“…Classical swine fever (CSF) is a highly contagious disease that affects domestic pigs and wild boars. CSF poses a threat for the pig industry from sanitary and economic points of view, and its notification is mandatory to the World Health Organization for animal health (OIE) [ 1 , 2 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

“…However, this approach carries significant ethical and economic implications. Meanwhile, in other countries where the virus remains endemic, vaccination poses a sustainable alternative to prevent massive losses and to contain the virus spread [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

“…The C-strain based vaccines are the most well studied ones, and their administration has proven to be safe and highly effective regardless of the genotype of the challenge strain [ 12 ]. However, CSFV persists in endemic countries even after active vaccination programs with these vaccines, mainly in areas that use either cell-culture or lapinized C-strain [ 2 ]. It is likely that this phenomenon is related to shortcomings in the vaccine program implementation rather than the effectiveness of the vaccines themselves [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Early and Solid Protection Afforded by the Thiverval Vaccine Provides Novel Vaccination Alternatives Against Classical Swine Fever Virus

et al. 2021

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Classical swine fever virus (CSFV) remains a challenge for the porcine industry. Inefficient vaccination programs in some endemic areas may have contributed to the emergence of low and moderate virulence CSFV variants. This work aimed to expand and update the information about the safety and efficacy of the CSFV Thiverval-strain vaccine. Two groups of pigs were vaccinated, and a contact and control groups were also included. Animals were challenged with a highly virulent CSFV strain at 21- or 5-days post vaccination (dpv). The vaccine induced rapid and strong IFN-α response, mainly in the 5-day immunized group, and no vaccine virus transmission was detected. Vaccinated pigs showed humoral response against CSFV E2 and Erns glycoproteins, with neutralising activity, starting at 14 days post vaccination (dpv). Strong clinical protection was afforded in all the vaccinated pigs as early as 5 dpv. The vaccine controlled viral replication after challenge, showing efficient virological protection in the 21-day immunized pigs despite being housed with animals excreting high CSFV titres. These results demonstrate the high efficacy of the Thiverval strain against CSFV replication. Its early protection capacity makes it a useful alternative for emergency vaccination and a consistent tool for CSFV control worldwide.

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“…CSFV can cause classical swine fever (CSF), another noti able disease to the OIE, which is characterized by high fever, leukopenia, extensive hemorrhage, convulsion and constipation or diarrhea, with high morbidity and mortality [8]. CSF was rst reported in Ohio, USA in 1833, and nowadays, CSF is still prevalent in many countries in the world [9,10]. Although Chinese C-strain vaccine of CSFV was developed in 1950s and has been widely using since then in the elds, CSF is still sporadic in many regions in China [11,12].…”

Section: Introductionmentioning

confidence: 99%

Development of A One-Step Multiplex qRT-PCR Assay For Detection of African Swine Fever Virus, Classical Swine Fever Virus And Atypical Porcine Pestivirus

Liu

Shi

Zhao

et al. 2021

Preprint

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Background: African swine fever virus (ASFV), classical swine fever virus (CSFV) and atypical porcine pestivirus (APPV) have caused great economic losses to the swine industry in China. Since there exist co-infections of ASFV, CSFV and APPV in certain pig herds, it is necessary to accurately and differentially detect these pathogens in the fields. In this study, a one-step multiplex real-time quantitative reverse transcription-polymerase chain reaction (multiplex qRT-PCR) was developed for simultaneous and differential detection of ASFV, CSFV and APPV.Results: The developed one-step multiplex qRT-PCR was able to specifically detect ASFV, CSFV and APPV, but could not amplify other viruses, including porcine circovirus type 2 (PCV2), pseudorabies virus (PRV), porcine reproductive and respiratory syndrome virus (PRRSV), foot-and-mouth disease virus (FMDV), porcine parvovirus (PPV), porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), porcine rotavirus (PRoV), porcine deltacoronavirus (PDCoV), border disease virus (BDV), bovine viral diarrhea virus type 1 (BVDV-1), BVDV-2 and so on. The detection limit of the assay was 2.52×101 copies/μL for ASFV, CSFV and APPV. Repeatability test using standard plasmids showed that the coefficients of variation of the intra- and inter-assay were less than 2 %. The detection of 509 clinical samples collected in Guangxi Province, Southern China from October 2018 to December 2020 showed that the positive rates of ASFV, CSFV and APPV were 45.58 %, 12.57 % and 3.54 %, respectively, while the co-infection rates of ASFV and CSFV, ASFV and APPV, CSFV and APPV were 4.91 %, 1.38 %, 0.98 %, respectively. Phylogenetic analysis based on the nucleotide sequences of partial ASFV p72 gene showed that all ASFV strains from Guangxi Province, Southern China belonged to genotype I and II.Conclusion: A one-step multiplex qRT-PCR with high specificity, sensitivity, accuracy and repeatability was successfully developed for simultaneous and differential detection of ASFV, CSFV and APPV.

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Classical swine fever virus: the past, present and future

Cited by 114 publications

References 334 publications

Potential for Protein Kinase Pharmacological Regulation in Flaviviridae Infections

Potential for Protein Kinase Pharmacological Regulation in Flaviviridae Infections

Early and Solid Protection Afforded by the Thiverval Vaccine Provides Novel Vaccination Alternatives Against Classical Swine Fever Virus

Development of A One-Step Multiplex qRT-PCR Assay For Detection of African Swine Fever Virus, Classical Swine Fever Virus And Atypical Porcine Pestivirus

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