Classical Swine Fever Virus N<sup>pro</sup>Interacts with Interferon Regulatory Factor 3 and Induces Its Proteasomal Degradation

Bauhofer, Oliver; Summerfield, Artur; Sakoda, Yoshihiro; Tratschin, Jon-Duri; Hofmann, Martin; Ruggli, Nicolas

doi:10.1128/jvi.02032-06

Cited by 183 publications

(175 citation statements)

References 83 publications

Supporting

Mentioning

161

Contrasting

Unclassified

Order By: Relevance

“…Therefore, it seems that antiviral activity of 3D8 scFv against CSFV is not mediated by the induction of IFN-a/b at least. Slight increase of IFN-b mRNA level upon CSFV (LOM strain) was observed by RT-PCR, in contrast to the reports in which IFN-a/b proteins were not detected in PK-15 cells [13,21].…”

Section: Discussioncontrasting

confidence: 50%

“…It is known that CSFV interfere with IFN-a/b production in the infected cells by virtue of two proteins (N pro and E rns ) of CSFV [13,14,21]. In order to investigate the possibility that antiviral activity of 3D8 scFv against CSFV was resulted from breaking the suppression of IFN-a/b induction, we analyzed mRNA levels of IFN-b in control PK-15 cells and C26 cells infected with CSFV.…”

Section: Discussionmentioning

confidence: 99%

“…CSFV is capable of avoiding the cellular anti-viral defense by suppressing production of IFN-a/b induction [13,14]. To know that reduction of viral RNA level in C26 was caused by induction of IFN-a/b, expression of IFN-b mRNA was measured.…”

Section: Antiviral Activity Of 3d8 Scfv Is Not Associated With Inductmentioning

confidence: 99%

See 2 more Smart Citations

An RNA-hydrolyzing recombinant antibody exhibits an antiviral activity against classical swine fever virus

Jun¹,

Pham²,

Lim³

et al. 2010

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

a b s t r a c tSome proteins with ribonuclease (RNase) activity have been shown to suppress viral replication. A wellcharacterized recombinant antibody, 3D8 single-chain variable fragment (3D8 scFv), has RNA-hydrolyzing and cell-penetrating activities. Here, we investigated antiviral activity of 3D8 scFv against classical swine fever virus (CSFV). In a cell line expressing 3D8 scFv (C26), intracellular RNA-hydrolysis activity was higher compared to control PK-15 cells and viral replication was strongly suppressed at the viral RNA level, with the evidence of independency of IFN-a/b induction. Exogenous treatment of 3D8 scFv, prior to or post-CSFV infection, was also shown to suppress CSFV replication at the viral RNA level. These observations suggest that antiviral activity of 3D8 scFv may be due to the intrinsic RNase activity of 3D8 scFv, which is capable of targeting viral RNA genomes or transcripts.

show abstract

Section: Discussioncontrasting

confidence: 50%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

An RNA-hydrolyzing recombinant antibody exhibits an antiviral activity against classical swine fever virus

Jun¹,

Pham²,

Lim³

et al. 2010

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

show abstract

“…IRF-3 is depleted by the viral protein N pro through proteasomal degradation. 6 The combined effect of N pro and E rns is necessary to adequately block IFN production and establish PI. Infection of fetuses with N pro -or E rns -deleted mutants of ncp BVDV alone was not sufficient to block establishment of fetal infection.…”

Section: Persistent Infectionmentioning

confidence: 99%

Bovine Viral Diarrhea Virus Infections

Brodersen

2014

Vet Pathol

View full text Add to dashboard Cite

Bovine viral diarrhea virus (BVDV) continues to be of economic significance to the livestock industry in terms of acute disease and fetal loss. Many of the lesions relating to BVDV infection have been well described previously. The virus is perpetuated in herds through the presence of calves that are persistently infected. Relationships between various species and biotypes of BVDV and host defenses are increasingly understood. Understanding of the host defense mechanisms of innate immunity and adaptive immunity continues to improve, and the effects of the virus on these immune mechanisms are being used to explain how persistent infection develops. The noncytopathic biotype of BVDV plays the major role in its effects on the host defenses by inhibiting various aspects of the innate immune system and creation of immunotolerance in the fetus during early gestation. Recent advances have allowed for development of affordable test strategies to identify and remove persistently infected animals. With these improved tests and removal strategies, the livestock industry can begin more widespread effective control programs.

show abstract

“…For CSFV, the cells responsible for producing large quantities of type I IFN have been identified as plasmacytoid dendritic cells (pDCs) (18)(19)(20).…”

mentioning

confidence: 99%

Type I and III IFNs Produced by Plasmacytoid Dendritic Cells in Response to a Member of theFlaviviridaeSuppress Cellular Immune Responses

Reid

Juleff

Windsor

et al. 2016

The Journal of Immunology

View full text Add to dashboard Cite

The pestivirus noncytopathic bovine viral diarrhea virus (BVDV) can suppress IFN production in the majority of cell types in vitro. However, IFN is detectable in serum during acute infection in vivo for ∼5–7 d, which correlates with a period of leucopoenia and immunosuppression. In this study, we demonstrate that a highly enriched population of bovine plasmacytoid dendritic cells (DCs) produced IFN in response to BVDV in vitro. We further show that the majority of the IFN produced in response to infection both in vitro and in vivo is type III IFN and acid labile. Further, we show IL-28B (IFN-λ3) mRNA is induced in this cell population in vitro. Supernatant from plasmacytoid DCs harvested postinfection with BVDV or recombinant bovine IFN-α or human IL-28B significantly reduced CD4+ T cell proliferation induced by tubercle bacillus Ag 85–stimulated monocyte-derived DCs. Furthermore, these IFNs induced IFN-stimulated gene expression predominantly in monocyte-derived DCs. IFN-treated immature DCs derived from murine bone marrow also had a reduced capacity to stimulate T cell proliferative responses to tubercle bacillus Ag 85. Immature DCs derived from either source had a reduced capacity for Ag uptake following IFN treatment that is dose dependent. Immunosuppression is a feature of a number of pestivirus infections; our studies suggest type III IFN production plays a key role in the pathogenesis of this family of viruses. Overall, in a natural host, we have demonstrated a link between the induction of type I and III IFN after acute viral infection and transient immunosuppression.

show abstract

Classical Swine Fever Virus N^proInteracts with Interferon Regulatory Factor 3 and Induces Its Proteasomal Degradation

Cited by 183 publications

References 83 publications

An RNA-hydrolyzing recombinant antibody exhibits an antiviral activity against classical swine fever virus

An RNA-hydrolyzing recombinant antibody exhibits an antiviral activity against classical swine fever virus

Bovine Viral Diarrhea Virus Infections

Type I and III IFNs Produced by Plasmacytoid Dendritic Cells in Response to a Member of theFlaviviridaeSuppress Cellular Immune Responses

Contact Info

Product

Resources

About

Classical Swine Fever Virus NproInteracts with Interferon Regulatory Factor 3 and Induces Its Proteasomal Degradation

Cited by 183 publications

References 83 publications

An RNA-hydrolyzing recombinant antibody exhibits an antiviral activity against classical swine fever virus

An RNA-hydrolyzing recombinant antibody exhibits an antiviral activity against classical swine fever virus

Bovine Viral Diarrhea Virus Infections

Type I and III IFNs Produced by Plasmacytoid Dendritic Cells in Response to a Member of theFlaviviridaeSuppress Cellular Immune Responses

Contact Info

Product

Resources

About

Classical Swine Fever Virus N^proInteracts with Interferon Regulatory Factor 3 and Induces Its Proteasomal Degradation