Class-Specific Antibody Determination against &lt;i&gt;Aspergillus fumigatus &lt;/i&gt;by Means of the Enzyme-Linked Immunosorbent Assay

Kauffman, H.F.; Heide, S. van der; Beaumont, F; Blok, H.J.; Vries, K. de

doi:10.1159/000234069

Cited by 18 publications

(5 citation statements)

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“…[23][24][25] The serum IgG levels were significantly higher but not IgA and IgM in CPA subjects, as previously described. 28,37,38 Unlike a previous study, we did not find a difference in the proportion of total B cells and switched or unswitched B cells. 20 The previous study had a retrospective study design and included non-consecutive subjects with different underlying diseases, including chronic obstructive pulmonary disease, connective tissue diseases, cancer survivors and others.…”

Section: Discussioncontrasting

confidence: 89%

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Innate and adaptive immune responses in subjects with CPA secondary to post‐pulmonary tuberculosis lung abnormalities

Chirumamilla,

Arora,

Kaur

et al. 2024

Mycoses

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BackgroundPost‐tuberculosis lung abnormality (PTLA) is the most common risk factor for chronic pulmonary aspergillosis (CPA), and 14%–25% of the subjects with PTLA develop CPA. The pathogenesis and the host immune response in subjects with PTLA who develop CPA need to be better understood.MethodsWe prospectively compared the innate and adaptive immune responses mounted by patients of PTLA with or without CPA (controls). We studied the neutrophil oxidative burst (by dihydrorhodamine 123 test), classic (serum C3 and C4 levels) and alternative (mannose‐binding lectin [MBL] protein levels) complement pathway, serum immunoglobulins (IgG, IgM and IgA), B and T lymphocytes and their subsets in subjects with PTLA with or without CPA.ResultsWe included 111 subjects (58 CPA and 53 controls) in the current study. The mean ± SD age of the study population was 42.6 ± 15.7 years. The cases and controls were matched for age, gender distribution and body weight. Subjects with CPA had impaired neutrophil oxidative burst, lower memory T lymphocytes and impaired Th‐1 immune response (lower Th‐1 lymphocytes) than controls. We found no significant difference between the two groups in the serum complement levels, MBL levels, B‐cell subsets and other T lymphocyte subsets.ConclusionSubjects with CPA secondary to PTLA have impaired neutrophil oxidative burst and a lower Th‐1 response than controls.

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Section: Discussioncontrasting

confidence: 89%

“…The serum IgG levels were significantly higher but not IgA and IgM in CPA subjects, as previously described 28,37,38 . Unlike a previous study, we did not find a difference in the proportion of total B cells and switched or unswitched B cells 20 .…”

Section: Discussioncontrasting

confidence: 50%

Innate and adaptive immune responses in subjects with CPA secondary to post‐pulmonary tuberculosis lung abnormalities

Chirumamilla,

Arora,

Kaur

et al. 2024

Mycoses

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“…This patient had positive result of A. fumigatus culture. This may be due to the ability of ICT to detect IgM in addition to IgG, as the presence of IgM was reported in up to 50% of CPA cases [ 18 , 19 , 20 ]. Elevated IgM levels in patients after surgery for aspergilloma was detected, while the IgG existed in low level [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

Performance of LDBio Aspergillus WB and ICT Antibody Detection in Chronic Pulmonary Aspergillosis

Setianingrum

Azahra

Abdullah

et al. 2021

JoF

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The detection of Aspergillus antibody has a key role in the diagnosis of chronic pulmonary aspergillosis. Western blot (WB) and immunochromatography (ICT) lateral flow detection of Aspergillus antibody can be used as confirmatory and screening assays but their comparative performance in TB patients is not known. This study investigated the performance of these assays among 88 post-tuberculosis patients with suspected CPA. Sensitivity, specificity, receiver operating curve (ROC), area under-curve (AUC) and the agreement between two assays were evaluated. Both WB and ICT showed good sensitivity (80% and 85%, respectively) for detection of Aspergillus antibodies. Substantial agreement (0.716) between these assays was also obtained. The highest AUC result (0.804) was achieved with the combination of WB and ICT. The global intensity of WB correlated with the severity of symptoms in CPA group (p = 0.001). The combination of WB and ICT may increase specificity in CPA diagnosis.

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“…As such, it is probable that an assay using a mixture of antigens could remain positive for IgM over an extended period of infection (11). One limitation of Aspergillus -specific IgM testing is poor specificity (28, 30, 34, 35); however, the ability of the LDBio Aspergillus ICT test to detect both IgG and IgM may overcome this constraint to detect variations in serological responses to CPA. This study did not evaluate the individual contributions of IgG and IgM from patient sera to the ICT results acquired, and there have been no published studies on the antibody class-specific performance of the Aspergillus ICT test, warranting further investigation.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of LDBio Aspergillus ICT Lateral Flow Assay for IgG and IgM Antibody Detection in Chronic Pulmonary Aspergillosis

2019

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Detecting Aspergillus-specific IgG is critical to diagnosing chronic pulmonary aspergillosis (CPA). Existing assays are often cost- and resource-intensive and not compatible with resource-constrained laboratory settings. LDBio Diagnostics has recently commercialized a lateral flow assay based on immunochromatographic technology (ICT) that detects Aspergillus antibodies (IgG and IgM) in less than 30 min, requiring minimal laboratory equipment. A total of 154 CPA patient sera collected at the National Aspergillosis Centre (Manchester, United Kingdom) and control patient sera from the Peninsula Research Bank (Exeter, United Kingdom) were evaluated. Samples were applied to the LDBio Aspergillus ICT lateral flow assay, and results were read both visually and digitally. Results were compared with Aspergillus IgG titers in CPA patients, measured by ImmunoCAP-specific IgG assays. For proven CPA patients versus controls, sensitivity and specificity for the LDBio Aspergillus ICT were 91.6% and 98.0%, respectively. In contrast, the routinely used ImmunoCAP assay exhibited 80.5% sensitivity for the same cohort (cutoff value, 40 mg of antigen-specific antibodies [mgA]/liter). The assay is easy to perform but challenging to read when only a very faint band is present (5/154 samples tested). The ImmunoCAP Aspergillus IgG titer was also compared with the Aspergillus ICT test line intensity or rate of development, with weak to moderate correlations. The Aspergillus ICT lateral flow assay exhibits excellent sensitivity for serological diagnosis of CPA. Quantifying IgG from test line intensity measurements is not reliable. Given the short run time, simplicity, and limited resources needed, the LDBio Aspergillus ICT is a suitable diagnostic tool for CPA in resource-constrained settings.

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Class-Specific Antibody Determination against <i>Aspergillus fumigatus </i>by Means of the Enzyme-Linked Immunosorbent Assay

Cited by 18 publications

References 5 publications

Innate and adaptive immune responses in subjects with CPA secondary to post‐pulmonary tuberculosis lung abnormalities

Innate and adaptive immune responses in subjects with CPA secondary to post‐pulmonary tuberculosis lung abnormalities

Performance of LDBio Aspergillus WB and ICT Antibody Detection in Chronic Pulmonary Aspergillosis

Evaluation of LDBio Aspergillus ICT Lateral Flow Assay for IgG and IgM Antibody Detection in Chronic Pulmonary Aspergillosis

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