Class-IIS restriction enzymes- a review

Szybalski, Waclaw; Kim, Sun C.; Hasan, Noaman; Podhajska, Anna J.

doi:10.1016/0378-1119(91)90605-b

Cited by 19 publications

(23 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Given the assay that is used to ®nd them, which detects any activity yielding a consistent DNA fragmentation pattern, it is no surprise that they come in a large variety of`¯avors'. Early on it was recognized that while then-normal Type II enzymes recognized palindromic sequences and cleaved symmetrically within them, the Type IIS enzymes cut outside their normally asymmetric sequences and differed in other interesting ways (14). We now know of additional enzymes that cleave on both sides of their recognition sequence (e.g.…”

Section: Type IImentioning

confidence: 99%

See 1 more Smart Citation

A Nomenclature for Restriction Enzymes, DNA Methyltransferases, Homing Endonucleases, and Their Genes

Rj¹,

Belford²,

Bester³

et al. 2004

Restriction Endonucleases

133

View full text Add to dashboard Cite

show abstract

Section: Type IImentioning

confidence: 99%

“…However, in most cases both strands are cleaved away from the recognition sequence, which therefore remains intact. These were the earliest sub-classes of the Type II restriction enzymes to be recognized (14).…”

Section: Type Iismentioning

confidence: 99%

A Nomenclature for Restriction Enzymes, DNA Methyltransferases, Homing Endonucleases, and Their Genes

Rj¹,

Belford²,

Bester³

et al. 2004

Restriction Endonucleases

133

View full text Add to dashboard Cite

show abstract

“…Coupled with the discovery and characterization of the FokI endonuclease [18][19][20][21][22], zinc finger-FokI fusion proteins were developed to form the first ZFNs in 1996 [23]. Contributing to the specificity with which ZFNs cleave DNA is the fact that FokI requires dimerization for cleavage activity and has weak native self-interaction [24,25].…”

Section: Zinc Finger Nucleasesmentioning

confidence: 99%

To Crispr And Beyond: The Evolution Of Genome Editing In Stem Cells

Chen

Knoepfler

2016

Regen. Med.

View full text Add to dashboard Cite

The goal of editing the genomes of stem cells to generate model organisms and cell lines for genetic and biological studies has been pursued for decades. There is also exciting potential for future clinical impact in humans. While recent, rapid advances in targeted nuclease technologies have led to unprecedented accessibility and ease of gene editing, biology has benefited from past directed gene modification via homologous recombination, gene traps and other transgenic methodologies. Here we review the history of genome editing in stem cells (including via zinc finger nucleases, transcription activator-like effector nucleases and CRISPR–Cas9), discuss recent developments leading to the implementation of stem cell gene therapies in clinical trials and consider the prospects for future advances in this rapidly evolving field.

show abstract

“…Subclass IIM is at the opposite extreme from other type II subclasses as it recognizes and cleavages methylated target sequences. The key distinguishing feature of type IIS is the cleavage position outside of the recognition sequence at a defined distance [49]. Subclass IIT is an example of a variation in the typical genetic organization of type II RM systems, as the endonuclease is composed of two different subunits.…”

Section: Type II Rmmentioning

confidence: 99%