Class II antigens on canine T lymphocytes

Doveren, R. F. C.; Buurman, Wim A.; Schütte, B.; Groenewegen, Gerard; Linden, C. J. Van Der

doi:10.1111/j.1399-0039.1985.tb00448.x

Cited by 51 publications

(9 citation statements)

References 38 publications

(16 reference statements)

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“…3 and 4). Our finding is consistent with previous studies that showed more than 90% of bottlenose dolphins’ [35] and beluga whales’ [36] peripheral blood cells within lymphocyte region express MHC class II, which is also observed in ungulates and carnivores such as swine [36], equine [37], canine [38], and feline [39]. Although, MHC class I is displayed on all human and mouse nucleated cells, we found that only a small percentage of dolphin cells expressed MHC class I (Fig.…”

Section: Discussionsupporting

confidence: 93%

Phenotyping and comparing the immune cell populations of free-ranging Atlantic bottlenose dolphins (Tursiops truncatus) and dolphins under human care

et al. 2017

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BackgroundStudies suggest that free-ranging bottlenose dolphins exhibit a suppressed immune system because of exposure to contaminants or microorganisms. However, due to a lack of commercially available antibodies specific to marine mammal immune cell surface markers, the research has been indecisive. The purpose of this study was to identify cross-reactive terrestrial-specific antibodies in order to assess the changes in the immune cell populations of dolphins under human care and free-ranging dolphins. The blood and PBMC fraction of blood samples from human care and free-ranging dolphins were characterized by H&E staining of cytospin slides and flow cytometry using a panel of terrestrial-specific antibodies.ResultsIn this study, we show that out of 65 terrestrial-specific antibodies tested, 11 were cross-reactive and identified dolphin immune cell populations within their peripheral blood. Using these antibodies, we found significant differences in the absolute number of cells expressing specific markers within their lymphocyte and monocyte fractions. Interestingly, the peripheral blood mononuclear cell profile of free-ranging dolphins retained an additional population of cells that divided them into two groups showing a low (<27%) or high (>56%) percentage of smaller cells resembling granulocytes.ConclusionsWe found that the cross-reactive antibodies not only identified specific changes in the immune cells of free-ranging dolphins, but also opened the possibility to investigate the causal relationship between immunosuppression and mortality seen in free-ranging dolphins.

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Section: Discussionsupporting

confidence: 93%

Phenotyping and comparing the immune cell populations of free-ranging Atlantic bottlenose dolphins (Tursiops truncatus) and dolphins under human care

et al. 2017

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“…The pattern of reactivity of 2D16 with B and T lymphocytes of rhesus macaques and sheep, and human lymphoid cell lines is in accord with the expected distribution of class I1 antigens on lymphocytes of man, mouse and bovine (Nadler et al 1981, Hammerling et al 1979, Spooner & Ferrone 1984, Lewin et al 1985 and is different from that found on lymphocytes of swine and dogs in that the majority of peripheral blood T lymphocytes as well as B lymphocytes express class I1 antigens in the latter species (Thistlewaite et al 1983, Deeg et al 1982, Iwaki et al 1983, Doveren et al 1985. In the case of the dog, it has not been emphasized that the level of expression of class I1 antigens on T lymphocytes is less than that on B lymphocytes; this was indicated by immunohistochemical staining of canine lymphoid tissues with 2D16, CIA and TH21A in this study.…”

Section: Discussionsupporting

confidence: 51%

“…The possibility of diffusion of reaction product from intensely stained adjacent non-lymphoid cells could not be excluded as an explanation of this phenomenom. However, a subpopulation of class I1 antigen bearing thymocytes has been described in mouse, man and dog (Krammer et al 1975, Williams et al 1980, Doveren et al 1985, hence it is possible that the staining reactions are a true indication of class I1 antigen expression. We were unable to unequivocally determine the identity of the intensely stained non-lymphoid cells in the thymic medulla with our single labeling technique.…”

Section: Discussionmentioning

confidence: 99%

Development and characterization of a monoclonal antibody to class II MHC antigens in rhesus macaques

1986

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The characterization of a monoclonal antibody with specificity for a monomorphic determinant on rhesus macaque class II antigens is described. This antibody, designated 2D16, is an IgG2b immunoglobulin which also displayed useful cross‐reactivity with lymphoreticular cells and cell lines of other species including man, bonnet and stumptail macaques, sheep, dog and horse. Limited polymorphism of the 2D16 epitope was observed in the dog.

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“…Eight functional genes and five pseudogenes have been identified to date for the class I and II regions. In particular, MHC class II molecules in the dog are constitutively expressed on the surface of Dendritic cells (DCs), macrophages, B cells, T cells, thymocytes and vascular endothelium (Doveren et al 1985;Mueller et al 2004). DCs and MHC class II molecules play crucial roles in regulating immune responses in skin (Delves and Roitt 2000).…”

Section: Discussionmentioning

confidence: 98%

Rapid detection of self-biting disease of mink by specific sequence-characterized amplified regions

Liu

Ning

Yang

et al. 2011

Journal of Forestry Research

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Self-biting disease occurred in most farmed fur animals in the world. The mechanism and rapid detection method of this disease has not been reported. We applied bulked sergeant analysis (BSA) in combination with RAPD method to analyze a molecular genetic marker linked with self-biting trait in mink group. The molecular marker was converted into sequence-characterized amplified regions (SCAR) marker for rapid detection of this disease. A single RAPD marker A8 amplified a specific band of 263bp in self-biting minks, which was designated as SRA8-250, and non-specific band of 315bp in both self-biting and healthy minks.The sequences of the bands exhibited 75% and 88% similarity to Canis familiarizes major histocompatibility complex (MHC) class II region and Macaca mulatta MHC class I region, respectively. A SCAR marker SCAR-A8 was designed for the specific fragment SRA8-250 and validated in 30 self-biting minks and 30 healthy minks. Positive amplification of SCAR-A8 was detected in 24 self-biting minks and 12 healthy minks. χ 2 test showed significant difference (p<0.01) in the detection rate between the two groups. This indicated that SRA8-250 can be used as a positive marker to detect self-biting disease in minks. Furthermore, the finding that self-biting disease links with MHC genes has significant implications for the mechanism of the disease.

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Class II antigens on canine T lymphocytes

Cited by 51 publications

References 38 publications

Phenotyping and comparing the immune cell populations of free-ranging Atlantic bottlenose dolphins (Tursiops truncatus) and dolphins under human care

Phenotyping and comparing the immune cell populations of free-ranging Atlantic bottlenose dolphins (Tursiops truncatus) and dolphins under human care

Development and characterization of a monoclonal antibody to class II MHC antigens in rhesus macaques

Rapid detection of self-biting disease of mink by specific sequence-characterized amplified regions

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