Class I small heat-shock protein gives thermotolerance in tobacco

Park, Soo Min; Hong, Choo Bong

doi:10.1078/0176-1617-00660

Cited by 38 publications

(15 citation statements)

References 15 publications

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“…One important mechanism responsible for the extra strong ability of plants to cope with environmental stresses has been cited from the heat shock response that accompanies the synthesis of heat shock proteins (HSPs), especially under high temperatures (Vierling, 1991;Queitsch et al, 2000;Park and Hong, 2001;Sun et al, 2002;Sung et al, 2003;Sanmiya et al, 2004). HSPs are grouped into five distinct classes based on molecular mass; their functions are most aptly regarded as molecular chaperones (Buchanan et al, 2000).…”

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confidence: 98%

Molecular cloning and expression pattern analyses of heat shock protein 70 genes fromNicotiana tabacum

Cho

Hong

2004

J. Plant Biol.

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We have isolated three genomic clones that code tobacco HSP70s, using a tobacco hsp70 cDNA clone as a probe. NtHSPTO-1, NtHSP70-2, and NtHSP70-3 contain full open reading frames of 653, 653, and 648 amino acid residues, respectively. All share three conserved regions, namely the C-terminal substrate binding domain, oligomerization domain, and N-terminal ATPase domain. In a comparison of their amino acid sequences, NtHSP70-1 and NtHSPTO-2 were very similar to each other, while NtHSPTO-3 showed significant differences, instead being highly homologous to the cytosolic HSP70 members of Arabidopsi$ thaliana and other plant species. Therefore, NtHSP70-1 and NtHSPTO-3 were chosen for further analyses. RNA blot hybridizations showed typical heat shock-responsive expression patterns, although their signal intensities differed significantly. Transcription of NtHSPTO-1 was also induced by dehydration stress and hormone treatments, such as BA, GA, and IAA, but that of NtHSPTO-3 was not.

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confidence: 98%

Molecular cloning and expression pattern analyses of heat shock protein 70 genes fromNicotiana tabacum

Cho

Hong

2004

J. Plant Biol.

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“…This was the first demonstration that manipulating the expression of a single heat shock gene could both increase and decrease thermotolerance in plants. Recently, it was reported that the constitutive expression of a tobacco cytosolic smHSP (Park & Hong 2002) and a tomato mitochondrial smHSP (Sanmiya et al . 2004) enhanced thermotolerance in transgenic tobacco.…”

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confidence: 99%

Introduction of the carrot HSP17.7 into potato (Solanum tuberosum L.) enhances cellular membrane stability and tuberization in vitro

Ahn

Zimmerman

2005

Plant Cell & Environment

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We have examined the ability of a carrot (Daucus carota L.) heat shock protein gene encoding HSP17.7 (DcHSP17.7) to confer enhanced heat tolerance to potato (Solanum tuberosum L.), a cool‐season crop. The DcHSP17.7 gene was fused to a 6XHistidine (His) tag to distinguish the engineered protein from endogenous potato proteins and was introduced into the potato cultivar ‘Désirée’ under the control of the cauliflower mosaic virus (CaMV) 35S promoter. Western analysis showed that engineered DcHSP17.7 was constitutively, but not abundantly, expressed in transgenic potato lines before heat stress. Leaves from multiple regenerated potato lines that contain the transgene exhibited significantly improved cellular membrane stability at high temperatures, compared with wild‐type and vector control plants. Transgenic potato lines also exhibited enhanced tuberization in vitro: under a condition of constant heat stress, at 29 °C, nodal sections of the transgenic lines produced larger and heavier microtubers at higher rates, compared to the wild type and vector controls. The dry weight and percentages of microtubers that were longer than 5 mm were up to three times higher in the transgenic lines. Our results suggest that constitutive expression of carrot HSP17.7 can enhance thermotolerance in transgenic potato plants. To our knowledge, this is the first study that shows that the thermotolerance of potato can be enhanced through gene transfer.

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“…Heat shock proteins (HSPs) have been Transgenic tobacco expressing class I cytosolic small HSP gene, TLHS1 showed up to two times higher cotyledon opening rates in comparison to the transgenic tobacco seedlings carrying the TLHS1 gene in antisense orientation at high temperature stresses of 40 °C and 45 °C for 1 to 4 h (Park and Hong, 2002). Expression of Athsp101 and sHSP17.7 into the basmati rice resulted in higher survival and better growth performance in the recovery phase following the heat stress (Agarwal et.…”

Section: Heat Shock Proteinsmentioning

confidence: 99%