Class I integrons containing a dhfrI trimethoprim resistance gene cassette in aquatic Acinetobacter spp.

Petersen, Andreas

doi:10.1016/s0378-1097(99)00563-7

Cited by 26 publications

(34 citation statements)

References 18 publications

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“…The association of integrons with various resistance gene cassettes in aquatic environments has been well documented [33][34][35][36][37], however there are few reports of integron and associated resistance genes in Pseudomonas spp. There is limited information on the occurrence of integrons and other resistance genes in bacteria of aquaculture origin in Australia [38,39].…”

Section: Discussionmentioning

confidence: 99%

Resistance Determinants of Pseudomonas Species from Aquaculture in Australia

Ndi¹

2012

J Aquac Res Development

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Section: Discussionmentioning

confidence: 99%

Resistance Determinants of Pseudomonas Species from Aquaculture in Australia

Ndi¹

2012

J Aquac Res Development

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“…If such integrons are mobilized onto Rplasmids, indirect selection could contribute to the maintenance of tetracycline resistance genes within the population. Only few researchers so far have addressed the incidence and spread of class 1 integrons and integron-associated resistance genes in environmental microorganisms (27,31,41).…”

Section: Discussionmentioning

confidence: 99%

“…Only few studies have to date addressed the prevalence of class 1 integrons among environmental bacteria (27,31), and to our knowledge, this is the first report of class 1 integrons within the widely distributed motile Aeromonas species.…”

mentioning

confidence: 88%

Incidence, Distribution, and Spread of Tetracycline Resistance Determinants and Integron-Associated Antibiotic Resistance Genes among Motile Aeromonads from a Fish Farming Environment

Schmidt¹,

Bruun²,

Dalsgaard³

et al. 2001

Appl Environ Microbiol

252

192

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A collection of 313 motile aeromonads isolated at Danish rainbow trout farms was analyzed to identify some of the genes involved in high levels of antimicrobial resistance found in a previous field trial (A. S. Schmidt, M. S. Bruun, I. Dalsgaard, K. Pedersen, and J. L. Larsen, Appl. Environ. Microbiol. 66:4908-4915, 2000), the predominant resistance phenotype (37%) being a combined oxytetracycline (OTC) and sulphadiazine/trimethoprim resistance. Combined sulphonamide/trimethoprim resistance (135 isolates) appeared closely related to the presence of a class 1 integron (141 strains). Among the isolates containing integrons, four different combinations of integrated resistance gene cassettes occurred, in all cases including a dihydrofolate reductase gene and a downstream aminoglycoside resistance insert (87 isolates) and occasionally an additional chloramphenicol resistance gene cassette (31 isolates). In addition, 23 isolates had "empty" integrons without inserted gene cassettes. As far as OTC resistance was concerned, only 66 (30%) out of 216 resistant aeromonads could be assigned to resistance determinant class A (19 isolates), D (n ‫؍‬ 6), or E (n ‫؍‬ 39); three isolates contained two tetracycline resistance determinants (AD, AE, and DE). Forty OTC-resistant isolates containing large plasmids were selected as donors in a conjugation assay, 27 of which also contained a class 1 integron. Out of 17 successful R-plasmid transfers to Escherichia coli recipients, the respective integrons were cotransferred along with the tetracycline resistance determinants in 15 matings. Transconjugants were predominantly tetA positive (10 of 17) and contained class 1 integrons with two or more inserted antibiotic resistance genes. While there appeared to be a positive correlation between conjugative R-plasmids and tetA among the OTCresistant aeromonads, tetE and the unclassified OTC resistance genes as well as class 1 integrons were equally distributed among isolates with and without plasmids. These findings indicate the implication of other mechanisms of gene transfer besides plasmid transfer in the dissemination of antibiotic resistance among environmental motile aeromonads.

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“…Class 1 integrons were the most common integrons found in Gram-negative bacteria such as Acinetobacter, especially in A. baumannii isolates (Gallego and Towner, 2001;Da Silva et al, 2002;Houang et al, 2003;Poirel et al, 2003;Segal et al, 2003;Nemec et al, 2004;Zarrilli et al, 2004; Abbott et al, 2005). Class 1 integrons have also been found in Acinetobacter strains of environmental origin (Petersen et al, 2000).…”

Section: Transposons and Integronsmentioning

confidence: 99%

Safety Assessment of Transgenic Organisms, Volume 4

2010

Harmonisation of Regulatory Oversight in Biotechnology

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Class I integrons containing a dhfrI trimethoprim resistance gene cassette in aquatic Acinetobacter spp.

Cited by 26 publications

References 18 publications

Resistance Determinants of Pseudomonas Species from Aquaculture in Australia

Resistance Determinants of Pseudomonas Species from Aquaculture in Australia

Incidence, Distribution, and Spread of Tetracycline Resistance Determinants and Integron-Associated Antibiotic Resistance Genes among Motile Aeromonads from a Fish Farming Environment

Safety Assessment of Transgenic Organisms, Volume 4

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