Class I defensins (BraDef) from broccoli (Brassica oleracea var. italica) seeds and their antimicrobial activity

Pacheco-Cano, Rubén D; Salcedo‐Hernández, Rubén; Casados‐Vázquez, Luz E.; Wróbel, Kazimierz; Bideshi, Dennis K.; Barboza‐Corona, José E.

doi:10.1007/s11274-020-2807-6

Cited by 6 publications

(4 citation statements)

References 49 publications

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“…43 BraDef (BraDef1 + BraDef2), another plant defensin, at 12.6 μg, reduced the hyphal growth of C. gloeosporioides by approximately 56% and caused membrane disruption. 44 Understanding the mechanism of action requires an understanding of the processes that occur during growth suppression. This knowledge is vital for the potential future application of these substances as microbicidal agents.…”

Section: Discussionmentioning

confidence: 99%

“…The defensin PsDef2 from Pinus sylvestris presents antifungal activity against Fusarium sporotrichiella and Saccharomyces cerevisiae , oomycota against Phytophthora gonapodyides and antibacterial activity; in addition, it presents inhibition of different α‐amylases of insects 43 . BraDef (BraDef1 + BraDef2), another plant defensin, at 12.6 μg, reduced the hyphal growth of C. gloeosporioides by approximately 56% and caused membrane disruption 44 …”

Section: Discussionmentioning

confidence: 99%

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Defensin‐like peptides from Capsicum chinense induce increased ROS, loss of mitochondrial functionality, and reduced growth of the fungus Colletotrichum scovillei

Resende,

de Oliveira Mello,

Zeraik

et al. 2024

Pest Management Science

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In the present study, we identified and characterized two defensin‐like peptides in an antifungal fraction obtained from Capsicum chinense pepper fruits and inhibited the growth of Colletotrichum scovillei, which causes anthracnose. AMPs were extracted from the pericarp of C. chinense peppers and subjected to ion exchange, molecular exclusion, and reversed‐phase in a high‐performance liquid chromatography system. We investigated the endogenous increase in reactive oxygen species (ROS), the loss of mitochondrial functioning, and the ultrastructure of hyphae. The peptides obtained from the G3 fraction through molecular exclusion chromatography were subsequently fractionated using reverse‐phase chromatography, resulting in the isolation of fractions F1, F2, F3, F4, and F5. The F1 fraction suppressed C. scovillei growth by 90, 70.4, and 44% at 100, 50, and 25 μg/mL, respectively. At 24 h, the IC50 and minimum inhibitory concentration were 21.5 μg/mL and 200 μg/mL, respectively. We found an increase in ROS, which may have resulted in an oxidative burst, loss of mitochondrial functioning, and cytoplasm retraction, as well as an increase in autophagic vacuoles. MS/MS analysis of the F1 fraction indicated the presence of two defensin‐like proteins, and we were able to identify the expression of three defensin sequences in our C. chinense fruit extract. The F1 fraction was also found to inhibit the activity of insect α‐amylases. In summary, the F1 fraction of C. chinense exhibits antifungal activity against a major pepper pathogen that causes anthracnose. These defensin‐like compounds are promising prospects for further research into antifungal and insecticide biotechnology applications.This article is protected by copyright. All rights reserved.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Defensin‐like peptides from Capsicum chinense induce increased ROS, loss of mitochondrial functionality, and reduced growth of the fungus Colletotrichum scovillei

Resende,

de Oliveira Mello,

Zeraik

et al. 2024

Pest Management Science

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“…The compound responsible for the antifungal activity of broccoli seed extracts is an antimicrobial peptide called broccoli napin [73]. Promising antimicrobial activity of broccoli-derived compounds, such as antimicrobial peptides and sulforaphane against various foodborne pathogens, including Staphylococcus aureus, Bacillus subtilis, and Salmonella typhimurium, enable their application as preservatives in the functional food industry [74,75]. Moreover, research shows that glucosinolates present in cruciferous byproducts possess antifungal and antibacterial while also having bioherbicidal, antioxidant, antimutagenic, and anticarcinogenic properties [76].…”

Section: Antimicrobial Propertiesmentioning

confidence: 99%

The Valorization of Wastes and Byproducts from Cruciferous Vegetables: A Review on the Potential Utilization of Cabbage, Cauliflower, and Broccoli Byproducts

Shinali,

Zhang,

Altaf

et al. 2024

Foods

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The management of vegetable waste and byproducts is a global challenge in the agricultural industry. As a commonly consumed vegetable crop, cruciferous vegetables marked higher amounts of wastage during their supply chain processes, with a significant contribution from cabbage, cauliflower, and broccoli. Therefore, the sustainable and resource-efficient utilization of discarded materials is crucial. This review explores potential applications of cruciferous vegetable waste and byproducts, spotlighting cabbage, cauliflower, and broccoli in food, medicinal, and other industries. Their significance of being utilized in value-added applications is addressed, emphasizing important biomolecules, technologies involved in the valorization process, and future aspects of practical applications. Cabbage, cauliflower, and broccoli generate waste and low-processing byproducts, including leaves, stems, stalks, and rot. Most of them contain high-value biomolecules, including bioactive proteins and phytochemicals, glucosinolates, flavonoids, anthocyanins, carotenoids, and tocopherols. Interestingly, isothiocyanates, derived from glucosinolates, exhibit strong anti-inflammatory and anticancer activity through various interactions with cellular molecules and the modulation of key signaling pathways in cells. Therefore, these cruciferous-based residues can be valorized efficiently through various innovative extraction and biotransformation techniques, as well as employing different biorefinery approaches. This not only minimizes environmental impact but also contributes to the development of high-value-added products for food, medicinal, and other related industries.

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“…For example, recently, Pachero-Cano et al studied the anti-listeria activity of defensins from broccoli seeds. In this study, the crude extract of purified defensins were studied, demonstrating their activity [95]. Most of the peptides of this superfamily were isolated from seeds, apart from other parts of plants [35,40].…”

Section: Compounds Exerting Anti-listeria Activity In Plant Extractsmentioning

confidence: 99%

Natural Antimicrobials: A Reservoir to Contrast Listeria monocytogenes

Ricci,

Lazzi,

Bernini

2023

Microorganisms

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Natural environments possess a reservoir of compounds exerting antimicrobial activity that are forms of defence for some organisms against others. Recently, they have become more and more attractive in the food sector due to the increasing demand for natural compounds that have the capacity to protect food from pathogenic microorganisms. Among foodborne pathogens, Listeria monocytogenes can contaminate food during production, distribution, or storage, and its presence is especially detected in fresh, raw food and ready-to-eat products. The interest in this microorganism is related to listeriosis, a severe disease with a high mortality rate that can occur after its ingestion. Starting from this premise, the present review aims to investigate plant extract and fermented plant matrices, as well as the compounds or mixtures of compounds produced during microbial fermentation processes that have anti-listeria activity.

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Class I defensins (BraDef) from broccoli (Brassica oleracea var. italica) seeds and their antimicrobial activity

Cited by 6 publications

References 49 publications

Defensin‐like peptides from Capsicum chinense induce increased ROS, loss of mitochondrial functionality, and reduced growth of the fungus Colletotrichum scovillei

Defensin‐like peptides from Capsicum chinense induce increased ROS, loss of mitochondrial functionality, and reduced growth of the fungus Colletotrichum scovillei

The Valorization of Wastes and Byproducts from Cruciferous Vegetables: A Review on the Potential Utilization of Cabbage, Cauliflower, and Broccoli Byproducts

Natural Antimicrobials: A Reservoir to Contrast Listeria monocytogenes

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