Class B CpG-ODN2006 is highly associated with IgM and antimicrobial peptide gene expression through TLR9 pathway in yellowtail Seriola lalandi

Angulo, Carlos; Alamillo, Erika; Hirono, Ikuo; Kondo, Hidehiro; Jirapongpairoj, Walissara; Pérez-Urbiola, Juan Carlos; Reyes‐Becerril, Martha

doi:10.1016/j.fsi.2018.03.025

Cited by 11 publications

(3 citation statements)

References 58 publications

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“…When administered to rock bream, CpG-1668 activates stronger protective effects against viral infection than other CpG-ODNs with GTCGTT-hexamer or with the same GACGTT-hexamer motif but with different nucleotide lengths (100). CpG-2006 and CpG2007, which contain three copies of the GTCGTT-hexamer motifs in 24 and 22 nucleotides, respectively, have been shown to induce immune responses in yellowtail ( Seriola quinqueradiata ), olive flounder, large yellow croaker ( Larimichthys crocea ), grass carp ( Ctenopharyngodon idella ), Nile tilapia ( Oreochromis niloticus ), and Atlantic salmon (108, 109, 112, 113, 116, 121). In olive flounder, CpG-2007 has better protection against Edwardsiella tarda infection than CpG-1668 (112).…”

Section: Sequence Of Cpg-odn For Tlr9 and Tlr21 Activation In Teleostsmentioning

confidence: 99%

Immunostimulatory Activities of CpG-Oligodeoxynucleotides in Teleosts: Toll-Like Receptors 9 and 21

Lai

Luo

et al. 2019

Front. Immunol.

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Toll-like receptors (TLRs) are pattern-recognition receptors that detect a wide variety of microbial pathogens for the initiation of host defense immunological responses. Thirteen TLRs have been identified in mammals, and teleosts contain 22 mammalian or non-mammalian TLRs. Of these, TLR9 and TLR21 are the cytosine-phosphate-guanosine-oligodeoxynucleotides (CpG-ODNs) recognition TLRs in teleosts. TLR9 is a mammalian TLR expressed in teleost but not in the avian species. TLR21 is a non-mammalian TLR expressed in both teleost and the avian species. Synthetic CpG-ODNs are potent immunostimulants that are being studied for their application against tumors, allergies, and infectious diseases, and as a vaccine adjuvant in humans. The immunostimulatory effects of CpG-ODNs as vaccine adjuvants and their antimicrobial function in domestic animals and teleosts are also being investigated. Most of our current knowledge about the molecular basis for the immunostimulatory activity of CpG-ODNs comes from earlier studies of the interaction between CpG-ODN and TLR9. More recent studies indicate that in addition to TLR9, TLR21 is another receptor for CpG-ODN recognition in teleosts to initiate immune responses. Whether these two receptors have differential functions in mediating the immunostimulatory activity of CpG-ODN in teleost has not been well-studied. Nevertheless, the existence of two recognition TLRs suggests that the molecular basis for the immunostimulatory activity of CpG-ODN in teleosts is different and more complex than in mammals. This article reviews the current knowledge of TLR9 and TLR21 activation by CpG-ODNs. The key points that need to be considered for CpG-ODNs as immunostimulants with maximum effectiveness in activation of immune responses in teleosts are discussed. This includes the structure/activity relationship of CpG-ODN activities for TLR9 and TLR21, the structure/functional relationship of these two TLRs, and differential expression levels and tissue distributions for these two TLRs.

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Section: Sequence Of Cpg-odn For Tlr9 and Tlr21 Activation In Teleostsmentioning

confidence: 99%

Immunostimulatory Activities of CpG-Oligodeoxynucleotides in Teleosts: Toll-Like Receptors 9 and 21

Lai

Luo

et al. 2019

Front. Immunol.

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“…As reported, IgM antibody plays an important role in the early period of anti-infection and its production could be promoted through activating the TLR9 pathway by CpG. 54,55 We collected serum of each mouse at 5 days post immunization, and the ELISA results showed that IgM antibody titers against S. f lexneri 2a strain 301 LPS increased in mice immunized with CTB-SA-OPS-CpG (Figure 5d). Subsequently, mice were abdominally challenged with approximately 1.68 × 10 7 CFU mouse −1 (2 × LD 50 ) of S. flexneri 2a strain 301 strains at 7 days post immunization.…”

Section: Evaluation Of Innate Immunity and Protective Effect Of The N...mentioning

confidence: 58%

“…Then, the expression levels of various TLR9 signaling pathway molecules in the lymph nodes of each mice were analyzed by the real-time quantitative polymerase chain reaction (RT-PCR) and the quantitative results revealed that the expression levels of TLR9 signaling molecules (including TLR9, Nfκb, Irf5, and Baff) were significantly increased in mice immunized with CTB-SA-OPS-CpG, compared with that in the CTB-SA-OPS+CpG group (Figure c), indicating that CpG coupled on the nanoparticle could better activate the TLR9 signaling pathway than free CpG. As reported, IgM antibody plays an important role in the early period of anti-infection and its production could be promoted through activating the TLR9 pathway by CpG. , We collected serum of each mouse at 5 days post immunization, and the ELISA results showed that IgM antibody titers against S. flexneri 2a strain 301 LPS increased in mice immunized with CTB-SA-OPS-CpG (Figure d). Subsequently, mice were abdominally challenged with approximately 1.68 × 10 7 CFU mouse –1 (2 × LD 50 ) of S. flexneri 2a strain 301 strains at 7 days post immunization.…”

Section: Resultsmentioning

confidence: 99%

Self-Assembled Proteinaceous Nanoparticles for Co-Delivery of Antigens and Cytosine Phosphoguanine (CpG) Adjuvants: Implications for Nanovaccines

Li¹,

Pan²,

Li³

et al. 2023

ACS Appl. Nano Mater.

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Nanotechnology has developed rapidly, giving rise to "nanovaccinology". In particular, protein-based nanocarriers have gained widespread attention because of their excellent biocompatibility. As the development of flexible and rapid vaccines is challenging, modular extensible nanoparticles are urgently needed. In this study, a multifunctional nanocarrier capable of delivering various biomolecules (including polysaccharides, proteins, and nucleic acids) was designed by fusing the cholera toxin B subunit with streptavidin. Then, the nanocarrier was used to prepare a bioconjugate nanovaccine against S. f lexneri by co-delivery of antigens and CpG adjuvants. Subsequent experimental results indicated that the nanovaccine with multiple components could stimulate both adaptive and innate immunity. Moreover, combining nanocarriers and CpG adjuvants with glycan antigens could improve the survival of vaccinated mice during the interval of two vaccination injections. The multifunctional nanocarrier and the design strategy demonstrated in this study could be utilized in the development of many other nanovaccines against infectious diseases.

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Additional use of α-IgM antibodies potentiates CpG ODN2006-induced B cell activation by targeting mainly naïve and marginal zone-like B cells

Fleige,

Fillatreau,

Claus

et al. 2024

Cellular Immunology

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Class B CpG-ODN2006 is highly associated with IgM and antimicrobial peptide gene expression through TLR9 pathway in yellowtail Seriola lalandi

Cited by 11 publications

References 58 publications

Immunostimulatory Activities of CpG-Oligodeoxynucleotides in Teleosts: Toll-Like Receptors 9 and 21

Immunostimulatory Activities of CpG-Oligodeoxynucleotides in Teleosts: Toll-Like Receptors 9 and 21

Self-Assembled Proteinaceous Nanoparticles for Co-Delivery of Antigens and Cytosine Phosphoguanine (CpG) Adjuvants: Implications for Nanovaccines

Additional use of α-IgM antibodies potentiates CpG ODN2006-induced B cell activation by targeting mainly naïve and marginal zone-like B cells

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