Class 1 non-symbiotic and class 3 truncated hemoglobin-like genes are differentially expressed in stone fruit rootstocks (Prunus L.) with different degrees of tolerance to root hypoxia

Almada, Rubén; Arismendi, María José; Pimentel, Paula; Rojas, Pamela; Hinrichsen, Patricio; Pinto, Manuel; Sagredo, Boris

doi:10.1007/s11295-013-0618-8

Cited by 24 publications

(23 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The signal is proportional to the amount of DNA polymerase chain product and the apparatus detects the accumulation of the amplified product during each cycle of the DNA polymerase reaction. Several studies were carried out with the RT-qPCR technique as to verify gene expression in Prunus rootstock under waterinduced stress, such as those performed by Pistelli et al (2012) and Almada et al (2013) in which the molecular responses in plants submitted to flooding were studied, whereas Jiménez et al (2013) studied the responses in plants under water-deficit stress.…”

Section: Introductionmentioning

confidence: 99%

Differential expression of the genes involved in responses to water-deficit stress in peach trees cv. Chimarrita grafted onto two different rootstocks

et al. 2019

View full text Add to dashboard Cite

Water deficit is responsible for a negative impact on agricultural systems. Several physiological, biochemical, and molecular processes are initiated as a response to water-deficit stress.Quantifying the differential expression of the genes involved in the response to water-deficit stress plays a key role in the development of molecular strategies for engineering water-stress tolerance in plants.Therefore, the present study aims to evaluate the expression of key genes involved in the water-deficit response process in peach trees cv. Chimarrita grafted onto two different rootstocks. The experimental design was completely randomized, with four treatments that correspond to the evaluation periods: day zero (control), the fourth, seventh, and ninth days of water-deficit stress. Leaf samples of each Chimarrita/rootstock combination were analyzed separately. The PLANT BREEDING -ArticleDifferential expression of the genes involved in responses to water-deficit stress in peach trees cv. Chimarrita grafted onto two different rootstocks expression of the genes related to ethylene biosynthesis, osmotic adjustment, and carbohydrate metabolism, namely ACC oxidase, GTL, SDH, SIP1, SOT1, S6PDH, and P5CS was quantified by real-time quantitative polymerase chain reaction (RT-qPCR). A differential response was observed in the expression of the SDH, GTL, P5CS, and SIP1 genes between Chimarrita/rootstock (Aldrighi 1 and Tsukuba 2) combinations. In both combinations of grafting, the S6PDH gene presented the highest level of expression at the fourth day of stress.These results show that genes related to carbohydrate and proline metabolism are important molecular markers to identify variability to water-deficit tolerance in Prunus persica.

show abstract

Section: Introductionmentioning

confidence: 99%

Differential expression of the genes involved in responses to water-deficit stress in peach trees cv. Chimarrita grafted onto two different rootstocks

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Saline, cold, heat and drought stress treatments were applied according to [18,19]. ‘Mariana 2624’ plants of uniform size (30 cm tall) were used for these experiments.…”

Section: Methodsmentioning

confidence: 99%

Identifying and validating housekeeping hybridPrunussp. genes for root gene-expression studies

Bastías

Oviedo

Almada

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

Prunus rootstock belonging to subgenera Amygdalus (peach), Prunus (plum) and Cerasus (cherry) are either from the same species as the scion or another one. The number of inter-species (including inter-subgenera) hybrids have increased as a result of efforts to broaden the genetic basis for biotic and abiotic resistance/tolerance. Identifying genes associated with important traits and responses requires expression analysis. Relative quantification is the simplest and most popular alternative, which requires reference genes (housekeeping) to normalize RT-qPCR data. However, there is a scarcity of validated housekeeping genes for hybrid Prunus rootstock species. This research aims to increase the number of housekeeping genes suitable for Prunus rootstock expression analysis.Twenty-one candidate housekeeping genes were pre-selected from previous RNAseq data that compared the response of root transcriptomes of two rootstocks subgenera to hypoxia treatment, ‘Mariana 2624’ (P. cerasifera Ehrh.× P. munsoniana W. Wight & Hedrick), and ‘Mazzard F12/1’ (P. avium L.). Representing groups of low, intermediate or high levels of expression, the genes were assayed by RT-qPCR at 72 hours of hypoxia treatment and analyzed with NormFinder software. A sub-set of seven housekeeping genes that presented the highest level of stability were selected, two with low levels of expression (Unknown 3, Unknown 7) and five with medium levels (GTB 1, TUA 3, ATPase P, PRT 6, RP II). The stability of these genes was evaluated under different stress conditions, cold and heat with the hybrid ‘Mariana 2624’ and N nutrition with the hybrids ‘Colt’ (P. avium × P. pseudocerasus Lindl.) and ‘Garnem’ [P. dulcis Mill.× (P. persica L.× P. davidiana Carr.)]. The algorithms of geNorm and BestKeeper software also were used to analyze the performance of these genes as housekeepers.Stability rankings varied according to treatments, genotypes and the software for evaluation, but the gene GBT 1 often had the highest ranking. However, most of the genes are suitable depending on the stressor and/or genotype to be evaluated. No optimal number of reference genes could be determined with geNorm software when all conditions and genotypes were considered. These results strongly suggest that relative RT-qPCR should be analyzed separately with their respective best housekeeper according to the treatment and/or genotypes in Prunus spp rootstocks.

show abstract

“…It has been reported that nsHb1 genes are induced by osmotic stress and high salinity (Taylor et al, 1994;Trevaskis et al, 1997;Lira-Ruan et al, 2001;Zhao et al, 2008;Almada et al, 2013), by treatments with nitrate, nitrite, nitric oxide, salicylic acid, methyl jasmonic acid, and H 2 O 2 (Wang et al, 2000;Sakamoto et al, 2004;Ohwaki et al, 2005;Qu et al, 2006;Sasakura et al, 2006), as well as by deficiencies in minerals such as P, K, and Fe (Wang et al, 2003). ZmHb mRNA levels in maize seedlings are induced by high-salt and osmotic stresses in addition to hypoxic stress (Zhao et al, 2008).…”

Section: Regulation By Osmotic Stress and Mineralsmentioning

confidence: 99%

“…It has been shown that expression of nsHb genes is modified when plants grow under different stress conditions, which suggests that the function of the nsHb genes is related to a plant's response to these stress conditions (Hebelstrup et al, 2006;Sairam et al, 2009;Almada et al, 2013). The AtnsHb1 (At21660) gene is expressed in roots of plants that grow under normal conditions, and is overexpressed in leaves and roots when plants are under hypoxic conditions (Hebelstrup et al, 2006;Dordas, 2009).…”

Section: Function Of Nshb In Stress Due To Hypoxia and Its Interactiomentioning

confidence: 99%

Non-symbiotic hemoglobin and its relation with hypoxic stress

Riquelme¹,

Hinrichsen

2015

Chilean J. Agric. Res.

Self Cite

View full text Add to dashboard Cite

Today we know that several types of hemoglobins exist in plants. The symbiotic hemoglobins were discovered in 1939 and are only found in nodules of plants capable of symbiotically fixing atmospheric N. Another class, called non-symbiotic hemoglobin, was discovered 32 yr ago and is now thought to exist throughout the plant kingdom, being expressed in different organs and tissues. Recently the existence of another type of hemoglobin, called truncated hemoglobin, was demonstrated in plants. Although the presence of hemoglobins is widespread in the plant kingdom, their role has not yet been fully elucidated. This review discusses recent findings regarding the role of plant hemoglobins, with special emphasis on their relationship to plants adaptation to hypoxia. It also discusses the role of nitric oxide in plant cells under hypoxic conditions, since one of the functions of hemoglobin appears to be modulating nitric oxide levels in the cells.

show abstract

Class 1 non-symbiotic and class 3 truncated hemoglobin-like genes are differentially expressed in stone fruit rootstocks (Prunus L.) with different degrees of tolerance to root hypoxia

Cited by 24 publications

References 48 publications

Differential expression of the genes involved in responses to water-deficit stress in peach trees cv. Chimarrita grafted onto two different rootstocks

Differential expression of the genes involved in responses to water-deficit stress in peach trees cv. Chimarrita grafted onto two different rootstocks

Identifying and validating housekeeping hybridPrunussp. genes for root gene-expression studies

Non-symbiotic hemoglobin and its relation with hypoxic stress

Contact Info

Product

Resources

About