Class 1 integrons in Dutch Salmonella enterica serovar Dublin isolates from clinical cases of bovine salmonellosis

Vo, An T. T.; Duijkeren, Engeline van; Fluit, Ad C.; Heck, Max; Verbruggen, Anjo; Zwaluw, Kim van der; Gaastra, Wim

doi:10.1016/j.vetmic.2006.05.001

Cited by 10 publications

(7 citation statements)

References 24 publications

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“…Reducing antibiotics use in pigs is particularly important to limit the emergence of multidrug resistance bacteria and to maintain good public health. According to previous reports (Chen et al 2008;Vo et al 2006b;Zhao et al 2007), Salmonella strains were highly resistant to ampicillin, chloramphenicol, kanamycin, streptomycin, sulfonamides, tetracyclines and quinolones, consistent with the results in this study. Among the 80 Salmonella isolates, 7 isolates were none drug resistant strains including 5 S. Newlands strains, one S. Derby and one S. Typhimurium.…”

Section: Discussionsupporting

confidence: 93%

Serotype distribution, antimicrobial susceptibility, antimicrobial resistance genes and virulence genes of Salmonella isolated from a pig slaughterhouse in Yangzhou, China

Yin

Zheng

et al. 2019

AMB Expr

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Salmonella is an important food-borne pathogen associated with public health and high economic losses. To investigate the prevalence and the characteristics of Salmonella in a pig slaughterhouse in Yangzhou, a total of 80 Salmonella isolates were isolated from 459 (17.43%) samples in 2016-2017. S. Derby (35/80, 43.75%) was the most prevalent, followed by S. Rissen (16/80, 20.00%) and S. Newlands (11/80, 13.75%). The highest rates of susceptibility were observed to cefoxitin (80/80, 100.0%) and amikacin (80/80, 100.0%), followed by aztreonam (79/80, 98.75%) and nitrofurantoin (79/80, 98.75%). The highest resistance rate was detected for tetracycline (65/80, 81.25%), followed by ampicillin (60/80, 75.00%), bactrim (55/80, 68.75%), and sulfisoxazole (54/80, 67.50%). Overall, 91.25% (73/80) of the isolates were resistant to at least one antibiotic, while 71.25% (57/80) of the isolate strains were multidrug resistant in the antimicrobial susceptibility tested. In addition, 86.36% (19/22) of the 22 antimicrobial resistance genes in the isolates were identified. Our data indicated that the resistance to certain antimicrobials was significantly associated, in part, with antimicrobial resistance genes. Furthermore, 81.25% (65/80) isolates harbored the virulence gene of mogA, of which 2 Salmonella Typhimurium isolates carried the mogA, spvB and spvC virulence genes at the same time. The results showed that swine products in the slaughterhouse were contaminated with multidrug resistant Salmonella commonly, especially some isolates carry the spv virulence genes. The virulence genes might facilitate the dissemination of the resistance genes to consumers along the production chain, suggesting the importance of controlling Salmonella during slaughter for public health.

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Section: Discussionsupporting

confidence: 93%

Serotype distribution, antimicrobial susceptibility, antimicrobial resistance genes and virulence genes of Salmonella isolated from a pig slaughterhouse in Yangzhou, China

Yin

Zheng

et al. 2019

AMB Expr

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“…Among the 113 strains studied and isolated over 33 years in Brazil, we found 14 different plasmid sizes (Figures and ). This diversity in the plasmids has been reported by other authors and several of them have been linked to virulence and resistance to anti‐microbials (Chu et al., ; Liebana et al., ; Lynne, Dorsey, David, & Foley, ; Vo et al., ). In the present study, the plasmids found were not related to anti‐microbial resistance profiles, to specific groups of the dendrograms generated or to the source of isolation being from humans or animals (Figures and ).…”

Section: Discussionsupporting

confidence: 75%

Genetic diversity, anti‐microbial resistance, plasmid profile and frequency of the Vi antigen in Salmonella Dublin strains isolated in Brazil

Vilela¹,

Frazão

Rodrigues

et al. 2017

Zoonoses and Public Health

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SummarySalmonella Dublin is strongly adapted to cattle causing enteritis and/or systemic disease with high rates of mortality. However, it can be sporadically isolated from humans, usually causing serious disease, especially in patients with underlying chronic diseases.The aim of this study was to molecularly type S. Dublin strains isolated from humans and animals in Brazil to verify the diversity of these strains as well as to ascertain possible differences between strains isolated from humans and animals. Moreover, the presence of the capsular antigen Vi and the plasmid profile was characterized in addition to the anti-microbial resistance against 15 drugs. For this reason, 113 S. Dublin strains isolated between 1983 and 2016 from humans (83) and animals (30) in Brazil were typed by PFGE and MLVA. The presence of the capsular antigen Vi was verified by PCR, and the phenotypic expression of the capsular antigen was determined serologically. Also, a plasmid analysis for each strain was carried out. The strains studied were divided into 35 different PFGE types and 89 MLVA-types with a similarity of ≥80% and ≥17.5%, respectively. The plasmid sizes found ranged from 2 to >150 kb and none of the strains studied presented the capsular antigen Vi. Resistance or intermediate resistance was found in 23 strains (20.3%) that were resistant to ampicillin, ciprofloxacin, chloramphenicol, imipenem, nalidixic acid, piperacillin, streptomycin and/or tetracycline. The majority of the S. Dublin strains studied and isolated over a 33-year period may descend from a common subtype that has been contaminating humans and animals in Brazil and able to cause invasive disease even in the absence of the capsular antigen. The higher diversity of resistance phenotypes in human isolates, as compared with animal strains, may be a reflection of the different anti-microbial treatments used to control S. Dublin infections in humans in Brazil. K E Y W O R D Santigen Vi, anti-microbial resistance, multiple-locus variable-number tandem-repeat analysis, plasmid analysis, pulsed-field gel electrophoresis, Salmonella Dublin

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“…The scheme of the conjugation experiments, in which the MDR Salmonella strains and the ESC-resistant E. coli or Klebsiella isolates could either act as donor or recipient, is shown in Table 2. Conjugation was carried out using the broth mating method (Vo et al, 2006b) except that the MacConkey agar was supplemented with a combination of ceftazidime (8 mg/ml) and kanamycin (256 mg/ml) or a combination of ceftazidime (8 mg/ml) and norfloxacin (32 mg/ ml) to select for transconjugants. The transconjugants were identified as Salmonella or E. coli using the API 20E system (bioMerieux, France).…”

Section: Conjugation Assaymentioning

confidence: 99%

Characteristics of extended-spectrum cephalosporin-resistant Escherichia coli and Klebsiella pneumoniae isolates from horses

Duijkeren

Fluit

et al. 2007

Veterinary Microbiology

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The aim of the present study was to contribute to the knowledge on extended-spectrum b-lactamases (ESBL's), AmpC blactamases and integrons in Enterobacteriaceae isolated from horses, which is still limited. The susceptibility of 1581 clinical isolates from animals to ceftiofur was tested. Most of these isolates (n = 1347) originated from horses. Seven ceftiofur-resistant equine isolates (four Escherichia coli and three Klebsiella pneumoniae) were identified and all seven were multidrug-resistant. These isolates were further studied for the presence of ESBL's, AmpC b-lactamases and class 1 integrons. The potential for the horizontal transfer of resistance genes among these clinical isolates was also studied. ESBL-type resistance genes were found in five isolates, AmpC-type genes in one isolates and integrons in six isolates. Nucleotide sequence analysis revealed that the isolates carried the bla CTX-M-1 , bla CMY-2 , bla TEM-1 and/or bla SHV-1 genes. This is the first report describing the in vitro conjugal transfer of the bla CTX-M-1 genes from a clinical E. coli isolate to Salmonella isolates. Gene cassettes encoding resistance to aminoglycosides (aadA1, aadA2 and aadA5), and trimethoprim (dfrA1, drfA12 and dfrA17) were found on the integrons present in the isolates. The cassette arrays of the dfrA17-aadA5 and dfrA1-aadA1 genes in the two integrons of a single E. coli isolate have not yet been described before. To our knowledge this is the first report on ESBL's and AmpC b-lactamases in equine E. coli and Klebsiella isolates. #

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Class 1 integrons in Dutch Salmonella enterica serovar Dublin isolates from clinical cases of bovine salmonellosis

Cited by 10 publications

References 24 publications

Serotype distribution, antimicrobial susceptibility, antimicrobial resistance genes and virulence genes of Salmonella isolated from a pig slaughterhouse in Yangzhou, China

Serotype distribution, antimicrobial susceptibility, antimicrobial resistance genes and virulence genes of Salmonella isolated from a pig slaughterhouse in Yangzhou, China

Genetic diversity, anti‐microbial resistance, plasmid profile and frequency of the Vi antigen in Salmonella Dublin strains isolated in Brazil

Characteristics of extended-spectrum cephalosporin-resistant Escherichia coli and Klebsiella pneumoniae isolates from horses

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