Class 1 integrons and resistance gene cassettes among multidrug resistant Salmonella serovars isolated from slaughter animals and foods of animal origin in Ethiopia

Molla, B.; Miko, Angelika; Pries, Karin; Hildebrandt, G.; Kleer, J.; Helmuth, Reiner

doi:10.1016/j.actatropica.2007.05.018

Cited by 35 publications

(28 citation statements)

References 25 publications

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“…More than 90% of these isolates were resistant to 2 to 12 antimicrobials. Similar results were described by other workers (33,35). The most common types of resistance found for these S. Saintpaul isolates were resistance to ampicillin (85% of the isolates), resistance to sulfamethoxazole (82%), resistance to nalidixic acid (79%), and resistance to streptomycin (75%); 34% and 25% of the isolates were resistant to gentamicin and kanamycin, whereas 44% and 49% of the isolates exhibited in- Dendrogram showing the genetic similarity between XbaI profiles determined by using the unweighted-pair group method with arithmetic averages (UPGMA) and Jaccard's coefficient (J).…”

Section: Discussionsupporting

confidence: 93%

“…Previous studies showed that isolates of S. Saintpaul are often multidrug resistant (33,35), but little is known about the mechanisms underlying antimicrobial resistance or about the pathogenic potential and epidemiology of isolates belonging to this serotype. The goals of this study were to obtain information about the resistance characteristics of isolates collected between 2000 and 2007 in Germany and to assess possible clonal relationships.…”

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confidence: 99%

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A Predominant Multidrug-Resistant Salmonella enterica Serovar Saintpaul Clonal Line in German Turkey and Related Food Products

Beutlich

Rodríguez

Käsbohrer

et al. 2010

Appl Environ Microbiol

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Recently, Salmonella enterica subsp. enterica serovar Saintpaul has increasingly been observed in several countries, including Germany. However, the pathogenic potential and epidemiology of this serovar are not very well known. This study describes biological attributes of S. Saintpaul isolates obtained from turkeys in Germany based on characterization of their pheno-and genotypic properties. Fifty-five S. Saintpaul isolates from German turkeys and turkey-derived food products isolated from 2000 to 2007 were analyzed by using antimicrobial agent, organic solvent, and disinfectant susceptibility tests, isoelectric focusing, detection of resistance determinants, plasmid profiling, pulsed-field gel electrophoresis (PFGE), and hybridization experiments. These isolates were compared to an outgroup consisting of 24 S. Saintpaul isolates obtained from humans and chickens in Germany and from poultry and poultry products (including turkeys) in Netherlands. A common core resistance pattern was detected for 27 German turkey and turkey product isolates. This pattern included resistance (full or intermediate) to ampicillin, amoxicillin-clavulanic acid, gentamicin, kanamycin, nalidixic acid, streptomycin, spectinomycin, and sulfamethoxazole and intermediate resistance or decreased susceptibility to ciprofloxacin (MIC, 2 or 1 g/ml, respectively) and several third-generation cephalosporins (including ceftiofur and cefoxitin [MIC, 4 to 2 and 16 to 2 g/ml, respectively]). These isolates had the same core resistance genotype, with bla TEM-1 , aadB, aadA2, sul1, a Ser833Glu83 mutation in the gyrA gene, and a chromosomal class 1 integron carrying the aadB-aadA2 gene cassette. Their XbaI, BlnI, and combined XbaIBlnI PFGE patterns revealed levels of genetic similarity of 93, 75, and 90%, respectively. This study revealed that a multiresistant S. Saintpaul clonal line is widespread in turkeys and turkey products in Germany and was also detected among German human fecal and Dutch poultry isolates.

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Section: Discussionsupporting

confidence: 93%

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confidence: 99%

A Predominant Multidrug-Resistant Salmonella enterica Serovar Saintpaul Clonal Line in German Turkey and Related Food Products

Beutlich

Rodríguez

Käsbohrer

et al. 2010

Appl Environ Microbiol

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“…Since the end of 2002, the emergence of multidrug-resistant and ciprofloxacin-resistant serovar Kentucky strains isolated from European travelers returning from African countries was reported in different studies (11,29,32,44). The SGI1-carrying serovar Kentucky strains studied here presented different MDR phenotypes with or without resistance to ciprofloxacin (Table 2).…”

Section: Discussionmentioning

confidence: 70%

Novel Insertion Sequence- and Transposon-Mediated Genetic Rearrangements in Genomic Island SGI1 ofSalmonella entericaSerovar Kentucky

Doublet

Praud

Bertrand

et al. 2008

Antimicrob Agents Chemother

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Salmonella genomic island 1 (SGI1) is an integrative mobilizable element that harbors a multidrug resistance (MDR) gene cluster. Since its identification in epidemic Salmonella enterica serovar Typhimurium DT104 strains, variant SGI1 MDR gene clusters conferring different MDR phenotypes have been identified in several S. enterica serovars and classified as SGI1-A to -O. A study was undertaken to characterize SGI1 from serovar Kentucky strains isolated from travelers returning from Africa. Several strains tested were found to contain the partially characterized variant SGI1-K, recently described in a serovar Kentucky strain isolated in Australia. This variant contained only one cassette array, aac(3)-Id-aadA7, and an adjacent mercury resistance module. Here, the uncharacterized part of SGI1-K was sequenced. Downstream of the mer module similar to that found in Tn21, a mosaic genetic structure was found, comprising (i) part of Tn1721 containing the tetracycline resistance genes tetR and tet(A); (ii) part of Tn5393 containing the streptomycin resistance genes strAB, IS1133, and a truncated tnpR gene; and (iii) a Tn3-like region containing the tnpR gene and the ␤-lactamase bla TEM-1 gene flanked by two IS26 elements in opposite orientations. The rightmost IS26 element was shown to be inserted into the S044 open reading frame of the SGI1 backbone. This variant MDR region was named SGI1-K1 according to the previously described variant SGI1-K. Other SGI1-K MDR regions due to different IS26 locations, inversion, and partial deletions were characterized and named SGI1-K2 to -K5. Two new SGI1 variants named SGI1-P1 and -P2 contained only the Tn3-like region comprising the ␤-lactamase bla TEM-1 gene flanked by the two IS26 elements inserted into the SGI1 backbone. Three other new variants harbored only one IS26 element inserted in place of the MDR region of SGI1 and were named SGI1-Q1 to -Q3. Thus, in serovar Kentucky, the SGI1 MDR region undergoes recombinational and insertional events of transposon and insertion sequences, resulting in a higher diversity of MDR gene clusters than previously reported and consequently a higher diversity of MDR phenotypes.The 43-kb Salmonella genomic island 1 (SGI1) is a Salmonella enterica-derived integrative mobilizable element that was originally identified in epidemic multidrug-resistant S. enterica serovar Typhimurium phage type DT104 strains (4). SGI1 contains a multidrug resistance (MDR) region containing all antibiotic resistance genes, which is a complex class 1 integron, recently named In104 and located in the 3Ј part of the island (Fig. 1) (4, 25). The In104 complex integron of SGI1 confers the common multidrug resistance profile to ampicillin, chloramphenicol-florfenicol, streptomycin-spectinomycin, sulfonamides, and tetracycline of DT104 isolates (4, 10, 34). The In104 complex integron possesses two cassette attachment sites (attI1). At the first attI1 site of this complex integron, the cassette carries the aadA2 gene, which confers resistance to streptomycin and spectinomycin,...

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“…One important cause of the proliferation of ARGs is the horizontal gene transfer (HGT) with mobile genetic elements (MGEs) as vectors, which have specific structures and ability to capture genes by a sitespecific recombination system. Integrons are a kind of MGEs linked with multidrug resistance and facilitate ARGs dissemination (Molla et al, 2007). Some bacteria can capture ARGs housed on the MGEs via HGT (Martinez, 2008) and Martinez et al (2014) ranked the ARGs which could transfer by MGEs harbored in human bacteria pathogens as the highest risk level.…”

Section: Introductionmentioning

confidence: 99%

Effects of chlortetracycline and copper on tetracyclines and copper resistance genes and microbial community during swine manure anaerobic digestion

Wang

Chen

Feng

et al. 2017

Bioresource Technology

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Class 1 integrons and resistance gene cassettes among multidrug resistant Salmonella serovars isolated from slaughter animals and foods of animal origin in Ethiopia

Cited by 35 publications

References 25 publications

A Predominant Multidrug-Resistant Salmonella enterica Serovar Saintpaul Clonal Line in German Turkey and Related Food Products

A Predominant Multidrug-Resistant Salmonella enterica Serovar Saintpaul Clonal Line in German Turkey and Related Food Products

Novel Insertion Sequence- and Transposon-Mediated Genetic Rearrangements in Genomic Island SGI1 ofSalmonella entericaSerovar Kentucky

Effects of chlortetracycline and copper on tetracyclines and copper resistance genes and microbial community during swine manure anaerobic digestion

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