Class 1 Integron in Pseudomonas aeruginosa Isolates From Different Places and Devices of ICU in Babol, Iran

Rajabnia, Ramazan; Asgharpour, Fariba; Ferdosi-Shahandashti, Elaheh; Khalilian, Mahia; Norkhomami, Sonia; Shafii, M; Moulana, Zahra

doi:10.5812/jjm.4850

Cited by 14 publications

(10 citation statements)

References 22 publications

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“…These data thus demonstrated that class I integrons are hugely important for the occurrence and transmission of MDR between bacteria. Rajabnia et al [27], in 2013, showed that out of 33 Pseudomonas aeruginosa samples isolated from different places and devices used in ICU, 39.4% had the int1 gene, 24.2% of which were characterized as MDR.In our study, despite the presence of int1 and int 2, there was no significant difference between antibiotic resistance and the presence of integrons in MDR E. coli isolates (p > 0.05). Integrons are located on plasmids and the rate of spread among bacteria is different and may cause different rates between bacterial samples.Márquez et al [28], in 2008 in Uruguay, reported that 15 of 104 E. coli strains had class 2 integrons.…”

Section: Discussioncontrasting

confidence: 57%

“…Different studies have shown that the prevalence of the class 1 integron is higher than the other classes of integron. Integrons and resistant genes that are located on plasmids can easily spread among bacteria species, and those which are located on gene cassettes can get separated and penetrate into other integrons [27]. Many antibiotics are prescribed and consumed widely in developed countries; this has also contributed to the emergence of resistant strains.…”

Section: Discussionmentioning

confidence: 99%

“…Many antibiotics are prescribed and consumed widely in developed countries; this has also contributed to the emergence of resistant strains. Bacteria serve as a reservoir for the dissemination of MDR, and this issue must be controlled to reduce the appearance or distribution of these antibiotic-resistant agents [18,27]. …”

Section: Discussionmentioning

confidence: 99%

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Multidrug-Resistant Escherichia coli Strains Isolated from Patients Are Associated with Class 1 and 2 Integrons

Lavakhamseh¹,

Mohajeri

Rouhi

et al. 2015

Chemotherapy

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Background:Escherichia coli isolates displaying multidrug-resistance (MDR) are a major health care problem that results in mortality and morbidity. Integrons are DNA elements in E.coli that are related to antibiotic resistance. The aim of this study was to determine class 1 and 2 integrons and MDR in E. coli isolates obtained from patients in two Sanandaj hospitals, located in Iran. Materials and Methods: 120 isolates of E. coli were obtained from clinical specimens (from November 2013 to April 2014), and the susceptibility of E. coli antimicrobial agents was determined using the Kirby-Bauer disk diffusion method according to the CLSI. PCR were applied for detection of class 1 and 2 integronsinE. coliisolates. SPSS software v16 and the χ² test were used for statistical analysis in order to calculate the association between antibiotic resistance and the presence of integrons (p < 0.05). Results: In a total of 120 E. coli isolates, 42.5% had MDR. Integrons were found in 50.9% of the MDR isolates, and included 47.05% class 1 and 3.92% class 2 integrons. The strains did not have both classes of integrons simultaneously. An association between resistance to antibiotics and integrons was found. Conclusion: Our results showed that int1 and int2 genes present in E. coli isolates obtained from patients cause MDR in this isolates. Since such bacteria are a reservoir for the transmission of MDR bacteria, appropriate programs are necessary to reduce this problem.

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Section: Discussioncontrasting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Multidrug-Resistant Escherichia coli Strains Isolated from Patients Are Associated with Class 1 and 2 Integrons

Lavakhamseh¹,

Mohajeri

Rouhi

et al. 2015

Chemotherapy

View full text Add to dashboard Cite

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“…But, in present study, regarding the 90% prevalence of Class 1 integrons as compared to their results, the prevalence rate was higher and no significant relationship was discovered between the presence of Class I integrons and the pattern of multidrug resistance genes ( p ˃ 0.05) [23]. Based on our results, the prevalence of Class I integrons in the present study was 90%, but in the study carried out by Rajabnia et al in Babol in 2013, the prevalence of Class I integrons was reported at 39.4% [24]. This is lower than our study, but this rate is high too.…”

Section: Discussioncontrasting

confidence: 71%

Evaluate the Relationship Between Class 1 Integrons and Drug Resistance Genes in Clinical Isolates of Pseudomonas aeruginosa

Hosseini¹,

Naeini²,

Khaledi³

et al. 2016

TOMICROJ

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Background:The prevalence of resistant Pseudomonas aeruginosa isolates is increasing and it is considered as one of the major public health concerns in the world. The association between integrons and drug resistance has been proven and evidences suggest that integrons are coding and responsible for dissemination of antibiotic resistance among P. aeruginosa isolates.Objective:This study is aimed to evaluate the relationship between class 1 integrons and drug resistance genes in clinical isolates of P. aeruginosa from burn patients.Methods:100 isolates of P. aeruginosa were collected from burn patients hospitalized in the skin ward of Shahid Motahari hospital and susceptibility testing was performed by disk diffusion method (Kirby-Bauer). Then DNA was extracted and PCR technique was performed for the detection of class 1 integrons and drug resistance genes. Then data was analyzed using SPSS software.Results:The most effective antibiotic was polymyxin B with sensitivity 100%, and the most resistance was observed to the ciprofloxacin (93%) and amikacin (67%), respectively. The maximum and lowest frequencies of drug resistance genes belonged to the aac (6 ') - 1, VEB-1 with prevalence rate 93% and 10%, respectively. The statistical Chi-square test did not find any significant correlation between class 1 integrons and drug resistance genes (p˃ 0.05).Conclusion:Although no significant correlation between class 1 integrons and drug resistance was observed, but the resistance rate to antibiotics tested among P. aeruginosa isolates was high. So, surveillance, optimization and strict consideration of antimicrobial use and control of infection are necessary.

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“…As compared to Class 1 and 2, less is known about Class 3 integron which have been described as rare and found only in a 2 limited number of isolates including Serratia marcescens, Klebsiella pneumoniae, P. aeruginosa, P. putida, Alcaligenes xylosoxidans, and Delftia spp (14). Cassettes usually include a single ORF and downstream, an attC site which is an imperfect inverted repeat sequence related to a 60bp consensus sequence (14)(15)(16).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Class 1, 2, and 3 Integrons and Biofilm Formation in Pseudomonas aeruginosa and Acinetobacter baumannii among ICU and non-ICU Patients

Aryanezhad¹,

Shakibaie²,

Karmostaji³

et al. 2016

IEM

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Background:Infections caused by Pseudomonas aeruginosa or Acinetobacter baumannii are of greatest concern for hospitalized patients, particularly those in intensive care units (ICUs). The aims of this study were to investigate the prevalence of integrons and biofilm formation among P. aeruginosa and A. baumannii isolates collected from ICU and non-ICU inpatients. Materials and Methods: A total of 90 P. aeruginosa and 90 A. baumannii isolates were recovered from patients admitted into diverse units of Shahid Mohammadi hospital in Bandar Abbas from January to December 2014. Bacterial identification was carried out by phenotypic methods and PCR. Antibiotic susceptibility was measured by disk diffusion assay. The presence of Class 1, 2, and 3 integrons were evaluated by multiplex-PCR. Biofilm quantification was done by microtiter method. Results: The highest number of isolates (48%) were recovered from ICU patients. About 81% of P. aeruginosa isolates were sensitive to piperacillin/tazobactam and ticarcillin, while 60% were resistant to third generation of cephalosporins. In case of A. baumannii, all the isolates were sensitive to colistin, but 98% were resistant to other antibiotics (p≤0.05). Susceptibility to ceftazidime, ticarcillin, imipenem, and piperacillin/tazobactam were higher among isolates obtained from non-ICU patients. Class 1 integron was detected in 13.3% of the P. aeruginosa and 40% of the A. baumannii isolates, while Class 2 integron was harbored by 7 and 6.6% of the isolates, respectively. Furthermore, 23% of the A. baumannii and 12% of the P. aeruginosa isolates showed strong biofilm activity. Conclusion: Class 1 integron-positive isolates were resistant to three classes of antibiotics and predominantly observed in specimens collected from ICU patients showing strong biofilm.

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Class 1 Integron in Pseudomonas aeruginosa Isolates From Different Places and Devices of ICU in Babol, Iran

Cited by 14 publications

References 22 publications

Multidrug-Resistant <b><i>Escherichia coli</i></b> Strains Isolated from Patients Are Associated with Class 1 and 2 Integrons

Multidrug-Resistant <b><i>Escherichia coli</i></b> Strains Isolated from Patients Are Associated with Class 1 and 2 Integrons

Evaluate the Relationship Between Class 1 Integrons and Drug Resistance Genes in Clinical Isolates of Pseudomonas aeruginosa

Prevalence of Class 1, 2, and 3 Integrons and Biofilm Formation in Pseudomonas aeruginosa and Acinetobacter baumannii among ICU and non-ICU Patients

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