CLASP Promotes Microtubule Rescue by Recruiting Tubulin Dimers to the Microtubule

Al-Bassam, Jawdat; Kim, Hwajin; Brouhard, Gary J.; Oijen, Antoine M. van; Harrison, Stephen C.; Chang, Fred

doi:10.1016/j.devcel.2010.07.016

Cited by 196 publications

(295 citation statements)

References 37 publications

(84 reference statements)

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“…Mini microtubule dynamics are regulated by an associated protein, BtubC, leading to stable, cytoskeletal rather than cytomotive filaments. The regulation of mini microtubules by BtubC is reminiscent of what certain microtubule associated proteins (MAPs), such as DCX, Clasp, and Mal3, do to microtubules (21)(22)(23).…”

mentioning

confidence: 99%

Four-stranded mini microtubules formed by Prosthecobacter BtubAB show dynamic instability

Deng

Fink

Bharat

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Microtubules, the dynamic, yet stiff hollow tubes built from αβ-tubulin protein heterodimers, are thought to be present only in eukaryotic cells. Here, we report a 3.6-Å helical reconstruction electron cryomicroscopy structure of four-stranded mini microtubules formed by bacterial tubulin-like Prosthecobacter dejongeii BtubAB proteins. Despite their much smaller diameter, mini microtubules share many key structural features with eukaryotic microtubules, such as an M-loop, alternating subunits, and a seam that breaks overall helical symmetry. Using in vitro total internal reflection fluorescence microscopy, we show that bacterial mini microtubules treadmill and display dynamic instability, another hallmark of eukaryotic microtubules. The third protein in the btub gene cluster, BtubC, previously known as “bacterial kinesin light chain,” binds along protofilaments every 8 nm, inhibits BtubAB mini microtubule catastrophe, and increases rescue. Our work reveals that some bacteria contain regulated and dynamic cytomotive microtubule systems that were once thought to be only useful in much larger and sophisticated eukaryotic cells.

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mentioning

confidence: 99%

Four-stranded mini microtubules formed by Prosthecobacter BtubAB show dynamic instability

Deng

Fink

Bharat

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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“…A common concept encountered in these cases is that the dynamic parameters of microtubules engaged in an overlap are locally altered by an effector protein recruited to the overlap by microtubule cross-linkers of the Ase1/PRC1/ MAP65 family. For instance, in yeast and human spindles the microtubule rescue factor CLASP is recruited (Bratman and Chang 2007;Liu et al 2009;Al-Bassam et al 2010) and in case of the animal midzone the growth-inhibiting kinesin KIF4 is recruited by PRC1 (Bieling et al 2010;Hu et al 2011;Nunes Bastos et al 2013). Large fluctuations in microtubule length are thus generally supressed in overlaps.…”

Section: Cell Plate Formationmentioning

confidence: 99%

Microtubule networks for plant cell division

2014

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During cytokinesis the cytoplasm of a cell is divided to form two daughter cells. In animal cells, the existing plasma membrane is first constricted and then abscised to generate two individual plasma membranes. Plant cells on the other hand divide by forming an interior dividing wall, the so-called cell plate, which is constructed by localized deposition of membrane and cell wall material. Construction starts in the centre of the cell at the locus of the mitotic spindle and continues radially towards the existing plasma membrane. Finally the membrane of the cell plate and plasma membrane fuse to form two individual plasma membranes. Two microtubule-based cytoskeletal networks, the phragmoplast and the pre-prophase band (PPB), jointly control cytokinesis in plants. The bipolar microtubule array of the phragmoplast regulates cell plate deposition towards a cortical position that is templated by the ring-shaped microtubule array of the PPB. In contrast to most animal cells, plants do not use centrosomes as foci of microtubule growth initiation. Instead, plant microtubule networks are striking examples of selforganizing systems that emerge from physically constrained interactions of dispersed microtubules. Here we will discuss how microtubule-based activities including growth, shrinkage, severing, sliding, nucleation and bundling interrelate to jointly generate the required ordered structures. Evidence mounts that adapter proteins sense the local geometry of microtubules to locally modulate the activity of proteins involved in microtubule growth regulation and severing. Many of the proteins and mechanisms involved have roles in other microtubule assemblies as well, bestowing broader relevance to insights gained from plants.

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“…CLASP1 is ubiquitously expressed, while CLASP2 is more abundant in the brain. CLASP1 and CLASP2 promote MT rescue and inhibit catastrophes (52,53).…”

Section: Microtubule Plus-end Tracking Proteins (+ Tips)mentioning

confidence: 99%

Role of non-motile microtubule-associated proteins in virus trafficking

Portilho

Persson²,

Arhel

2016

Biomolecular Concepts

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Viruses are entirely dependent on their ability to infect a host cell in order to replicate. To reach their site of replication as rapidly and efficiently as possible following cell entry, many have evolved elaborate mechanisms to hijack the cellular transport machinery to propel themselves across the cytoplasm. Long-range movements have been shown to involve motor proteins along microtubules (MTs) and direct interactions between viral proteins and dynein and/or kinesin motors have been well described. Although less well-characterized, it is also becoming increasingly clear that non-motile microtubule-associated proteins (MAPs), including structural MAPs of the MAP1 and MAP2 families, and microtubule plus-end tracking proteins (+ TIPs), can also promote viral trafficking in infected cells, by mediating interaction of viruses with filaments and/or motor proteins, and modulating filament stability. Here we review our current knowledge on nonmotile MAPs, their role in the regulation of cytoskeletal dynamics and in viral trafficking during the early steps of infection.

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CLASP Promotes Microtubule Rescue by Recruiting Tubulin Dimers to the Microtubule

Cited by 196 publications

References 37 publications

Four-stranded mini microtubules formed by Prosthecobacter BtubAB show dynamic instability

Four-stranded mini microtubules formed by Prosthecobacter BtubAB show dynamic instability

Microtubule networks for plant cell division

Role of non-motile microtubule-associated proteins in virus trafficking

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