Clarithromycin Resistance and 23S rRNA Mutations in Helicobacter pylori

Kargar, Mohammad; Baghernejad, Maryam; Doosti, Abbas; Ghorbani-Dalini, Sadegh

doi:10.5772/22655

Cited by 11 publications

(13 citation statements)

References 52 publications

(50 reference statements)

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“…In these studies, point mutations at positions 2142 or 2143 of the 23S rRNA gene have been identified. [2223] Point mutations identified in our study were different with those studies in Iran. In comparison with the sequence of strain u27270 H. pylori , we found one new point mutation at position T2243C (GenBank KC417289).…”

Section: Discussioncontrasting

confidence: 85%

The study of mutation in 23S rRNA resistance gene of Helicobacter pylori to clarithromycin in patients with gastrointestinal disorders in Isfahan - Iran

et al. 2014

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Background:Helicobacter pylori antimicrobial resistance is an important factor responsible for treatment failure. The purpose of this study was evaluating the prevalence of point mutations in clarithromycin-resistant clinical isolates of H. pylori in Isfahan city of Iran.Materials and Methods:Thirty isolates of H. pylori from 130 biopsy specimens were isolated by culture and confirmed by biochemical and PCR tests. The MIC of clarithromycin antibiotic for 30 clinical isolates of H. pylori was determined by E-test method. The point mutations in the 288 bp of 23S rRNA gene of H. pylori were investigated in four clarithromycin-resistant clinical isolates by PCR followed by sequencing.Results:Among 30 isolates of H. pylori, 4 cases were resistant to clarithromycin. One point mutation was found at position T2243C in the 23S rRNA gene in all resistance isolates.Conclusions:In our study, H. pylori resistance to clarithromycin associated with point mutation at position 2243 (T2243C).

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Section: Discussioncontrasting

confidence: 85%

The study of mutation in 23S rRNA resistance gene of Helicobacter pylori to clarithromycin in patients with gastrointestinal disorders in Isfahan - Iran

et al. 2014

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“…For instance, in several studies performed in Tehran, capital of Iran, resistance rate to clarithromycin was as follows: in two studies, Fallahi et al and Falsafi et al reported the rate of resistance to clarithromycin as 4.16% and 21%, respectively (3,28 (34). In Isfahan, Khashei et al conducted a study that reported 6.25% of resistance (23).…”

Section: Discussionmentioning

confidence: 99%

“…Several studies were done in Japan by their researchers during 2002 and 2011,reporting that the frequency of clarithromycin resistant strains was 11-12% (2002), 83.9% (2004) and 36.1% (2010) and ultimately it reached 38.8% in 2011 (16,34,40,41). In other studies in Poland, rate of resistance to clarithromycin, evaluated during 1998, 2005 and 2008 had an increasing trend form 9.1% to28% and 18.4%, toward to 1998, respectively (39,42,43).…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Resistance of Helicobacter pylori Strains to Selected Antibiotics in Isfahan, Iran

Mirzaei

Poursina

Faghri

et al. 2013

Jundishapur J Microbiol

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Background:Helicobacter pylori (H. pylori) is one of the most common infectious diseases in the world. It colonizes about 50-60% of the world's population. The estimated prevalence of H. pylori infection is approximately 65% in Iran. Objectives: The aim of the present study was to investigate the antimicrobial resistance of H. pylori to metronidazole, clarithromycin and amoxicillin. Materials and Methods: A total of 110 biopsy specimens were collected from patients with clinical symptoms of gastrointestinal disorders who were referred to the endoscopy unit at Al-Zahra Hospital, Isfahan, Iran. We investigated the frequency of H. pylori resistance to metronidazole, clarithromycin and amoxicillin among 48 positive isolates of H. pylori from the referred patients. After the culture of biopsy specimens and identification of H. pylori strains, susceptibility tests were done using the E test (epsilometer test) Results: The overall rates of resistance to metronidazole, clarithromycin and amoxicillin were 56.3%, 14.6% and 4.2% respectively. In our study, there was a significant correlation between resistance to metronidazole and gender (P < 0.05) but there was no significant correlation between resistance to amoxicillin and clarithromycin and gender (P ˃ 0.05). Conclusions: Information on antibiotic susceptibility profile plays an important role in empiric antibiotic treatment and management of refractive cases. With regards to this study, using metronidazole in the Isfahan region, can lead to eradication failure in clinical therapies due to having the highest rate of resistance but amoxicillin and clarithromycin are prescribed for first and second lines of treatment against H. pylori. Actually, susceptibility testing of H. pylori isolates in different geographical areas is advised because it aids the selection of optimal therapy regimens.

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“…19-25 41 Several reports have been published regarding RT-LAMP assays 42 that have utilized the HCV 5 0 non-coding region (5 0 -NCR) for 43 primer design and the detection of HCV-RNA. [22][23][24][25] Unfortunately, 44 these works have been limited to detection only and have 45 demonstrated no pattern formation of the bands, thereby 46…”

Section: Q2mentioning

confidence: 99%

A method for rapid detection and genotype identification of hepatitis C virus 1–6 by one-step reverse transcription loop-mediated isothermal amplification

Nyan

Swinson

2016

International Journal of Infectious Diseases

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Clarithromycin Resistance and 23S rRNA Mutations in Helicobacter pylori

Cited by 11 publications

References 52 publications

The study of mutation in 23S rRNA resistance gene of Helicobacter pylori to clarithromycin in patients with gastrointestinal disorders in Isfahan - Iran

The study of mutation in 23S rRNA resistance gene of Helicobacter pylori to clarithromycin in patients with gastrointestinal disorders in Isfahan - Iran

Prevalence of Resistance of Helicobacter pylori Strains to Selected Antibiotics in Isfahan, Iran

A method for rapid detection and genotype identification of hepatitis C virus 1–6 by one-step reverse transcription loop-mediated isothermal amplification

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