Cladia aggregata (lichen) from Brazilian northeast: chemical characterization and antimicrobial activity

Martins, Mônica Cristina Barroso; Lima, Marcio James Gonçalves de; Silva, Flávia Pereira; Azevedo-Ximenes, E.; Silva, Nicácio Henrique da; Pereira, Eugênia C.

doi:10.1590/s1516-89132010000100015

Cited by 34 publications

(19 citation statements)

References 24 publications

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“…Besides that, the bacteria strains are also different between this investigation and Yilmaz et al [23]. Martins et al [25] reported MIC values of 50-100 µg/mL for (2) against various strains of S. aureus which is similar to this study where we obtained MIC value of 62.50 µg/mL concentration. Disk diffusion method has its own disadvantages where zone sizes are affected by the media used and by the different growth rates of organisms being tested therefore MIC tests are more accurate with anaerobic bacteria [26].…”

Section: Notes: a Acetone Extract; M Methanol Extractsupporting

confidence: 83%

Antibacterial and Antioxidant Activity of Lichens Usnea rubrotincta, Ramalina dumeticola, Cladonia verticillata and Their Chemical Constituents

Gunasekaran¹,

Pillai²,

Ramanathan³

et al. 2016

MJAS

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The present study was carried out to evaluate the antibacterial and antioxidant activity of extract and chemical constituents of Usnea rubrotincta, Ramalina dumeticola and Cladonia verticillata. Acetone extract of U. rubrotincta showed promising antibacterial activity against Gram positive bacteria Bacillus subtilis with the lowest Minimal Inhibitory Concentration (MIC) value of (15.63 µg/mL). Six secondary metabolites were isolated using Preparative High Performance Liquid Chromatography (PHPLC) method from the two bioactive lichens U. rubrotincta and R. dumeticola (compound 1 -6). Among all six compounds, compound (1) exhibited strongest activity against both the tested Gram positive bacteria at 7.81 µg/mL. Compound (6) had 69.57% scavenging activity against DPPH free radical while the rest only showed below 50% scavenging activity. This is the first evaluation of antibacterial activity of lichens found in Malaysia and to our knowledge, this is the first report of antibacterial and antioxidant activity of compound (3) and (5). Keywords: antibacterial, antioxidant, Usnea rubrotincta, Ramalina dumeticola, Cladonia verticillata, usnic acid AbstrakKajian ini dijalankan bagi menilai aktiviti antibakteria dan antioksidan sebatian kimia liken yang diekstrak iaitu Usnea rubrotincta, Ramalina dumeticola dan Cladonia verticillata. Ekstrak aseton terhadap U. rubrotincta telah menunjukkan aktiviti antibakteria yang memberangsangkan terhadap bakteria Gram positif Bacillus subtilis dengan nilai Kepekatan Perencatan Minimum (KPM) sebanyak (15.63 µg/mL). Enam metabolit sekunder telah dipencilkan daripada 2 liken bioaktif iaitu U. rubrotincta dan R. dumeticola menggunakan kaedah Kromatografi Cecair Prestasi Tinggi Preparatif (KCPTP) (sebatian 1 -6). Daripada 6 sebatian, sebatian (1) telah memaparkan aktiviti yang paling kuat terhadap kedua-dua bakteria Gram positif yang dikaji pada kepekatan 7.81 µg/mL. Sebatian (6) mempunyai peratus pemerangkapan radikal bebas DPPH pada 69.57% manakala sebatian lain hanya menunjukkan aktiviti pemerangkapan kurang daripada 50%. Ini adalah penilaian pertama terhadap aktiviti antibakteria bagi liken yang dijumpai di Malaysia dan kepada pengetahuan kita, ini adalah laporan pertama bagi aktiviti antibakteria dan antioksidan sebatian (3) dan (5).

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Section: Notes: a Acetone Extract; M Methanol Extractsupporting

confidence: 83%

Antibacterial and Antioxidant Activity of Lichens Usnea rubrotincta, Ramalina dumeticola, Cladonia verticillata and Their Chemical Constituents

Gunasekaran¹,

Pillai²,

Ramanathan³

et al. 2016

MJAS

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“…; it can be conceived that the Cladia extract will also be effective against other anthropophilic and zoophilic spp., namely, M. pachydermatis, M. japonica, M. yamatoensis and M. equii. The C. aggregata , along with Usnea baileyi and Everniastrum nepalense , has been found active against multidrug resistant Staphylococcus aureus [31, 32]. Established results on the antifungal activity of Everniastrum cirrhatum with minimum fungicidal concentration (MFC) of as low as 60 μ L/mL against human pathogenic fungi (dermatophytes), namely, Epidermophyton floccosum, Microsporum gypseum, M. canis, M. audounii, Trichophyton rubrum, T. mentagrophytes, T. violaceum, and T. tonsurans, have also been reported in the past [33].…”

Section: Resultsmentioning

confidence: 99%

Management of Cosmetic Embarrassment Caused byMalasseziaspp. with Fruticose LichenCladiaUsing Phylogenetic Approach

Pandey

Mishra

Tiwari

et al. 2013

BioMed Research International

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During anti-Malassezia screening of plants by CLSI broth microdilution method, Cladia aggregata (Swartz) Nyl. (family Cladoniaceae), a fruticose lichen from Sikkim (northeast Himalayan region), has been found effective at minimum inhibitory concentrations (mg/mL) of 2.72, 0.63, and 1.28 against yeast-like fungi namely, M. furfur, M. globosa and M. sympodialis, respectively. These test pathogens are responsible for pityriasis versicolor (PV) and seborrheic dermatitis (SD) in humans. We tried to establish the reason for variable MICs against various Malassezia spp. using bioinformatical tools, thereby reducing the cost of the experimentation. This is the first report on anti-Malassezia activity of C. aggregata and thus can serve as a potential source for the development of cosmaceuticals.

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“…Ribeiro et al (2006) also reported action of ether extract from C. substellata against human and plant pathogenic bacteria and supposed a synergic action between their components. Martins et al (2010) also showed a synergic action of organic extract of C. aggregata against S. aureus. Similar results about the synergism between lichen substances have been observed for ether extract of Heterodermia leucomela, that contained high content of ATR and zeorin as an accessory substance, against B. subtilis (Falcã o et al, 2002).…”

Section: In Vitro Antioxidant and Antimicrobial Properties Of Organicmentioning

confidence: 87%

Production of Bioactive Lichen Compounds by Alginate-Immobilized Bionts Isolated from Cladonia verticillaris: An in Vitro Study

Santiago¹,

Martins²,

Nascimento³

et al. 2020

JPS

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Bionts isolated from thalli of Cladonia verticillaris, immobilized in calcium alginate, produce two depsidones, fumarprotocetraric and protocetraric acids, and the depside atranorin, that exhibit antimicrobial activity against Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae. Organic lichen extract (acetone/ether/chloroform) shows the highest antimicrobial activity due to a possible synergism between these substances. Antioxidant capacity of soluble metabolites secreted from the immobilisates to the bath medium of incubation during the first 12 days of immobilization has been found (˃80% oxidation inhibition). The concentration of soluble phenolic substances depends on the immobilization time (during 32 days), exogenous supply of acetate (1.0m mM sodium or calcium acetate) and on the type of isolated biont (phycobionts, mycobionts, whole thallus or immobilized phycobionts co-incubated with the mycobionts ones). The role of phycobionts in phenol production has been interpreted as a possible modification of the polymalonyl pathway; for example, atranorin is actively produced and secreted during immobilization while it is not detected in thallus in natura. Co-incubated bionts secrete higher amounts of atranorin to the media during the first 12 days of immobilization. Immobilization of isolated bionts could be used as a biotechnological technique to obtain a potential source of biological active compounds. On the other hand, the physiological state detected of C. verticillaris in the Cerrado is much better for experimentation than that of the specimens collected in Caatinga since, in this case, the thalli showed the fragility that the extreme environmental conditions of the semi-arid region of NE Brazil impose on this lichen species. To date, no priority has been defined for lichen conservation in Brazilian ecosystems. It is therefore suggested that it imust important to include lichen ecophysiology studies in public conservation policies.

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Cladia aggregata (lichen) from Brazilian northeast: chemical characterization and antimicrobial activity

Abstract: ABSTRACT

Cited by 34 publications

References 24 publications

Antibacterial and Antioxidant Activity of Lichens Usnea rubrotincta, Ramalina dumeticola, Cladonia verticillata and Their Chemical Constituents

Antibacterial and Antioxidant Activity of Lichens Usnea rubrotincta, Ramalina dumeticola, Cladonia verticillata and Their Chemical Constituents

Management of Cosmetic Embarrassment Caused byMalasseziaspp. with Fruticose LichenCladiaUsing Phylogenetic Approach

Production of Bioactive Lichen Compounds by Alginate-Immobilized Bionts Isolated from Cladonia verticillaris: An in Vitro Study

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