CKB1 is involved in abscisic acid and gibberellic acid signaling to regulate stress responses in Arabidopsis thaliana

Yuan, Congying; Ai, Jianping; Chang, Huoy-Rou; Xiao, Wenjun; Liu, Lu; Zhang, Cheng; Zhuang, He; Huang, Ji; Li, Jinyan; Guo, Xinhong

doi:10.1007/s10265-017-0924-6

Cited by 16 publications

(11 citation statements)

References 64 publications

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“…Recent molecular genetics and biochemical analyses suggest that CK2 is involved in ABA signaling and abiotic stress responses (for review: Vilela et al 2015b). So far, studies of CK2 knockout mutants and RNAi lines suggest that CK2αs and CK2β1 are positive regulators of ABA responses (Mulekar et al 2012; Mulekar and Huq 2014; Wang et al 2014; Yuan et al 2017), whereas biochemical studies suggest that CK2 negatively regulates SnRK2-mediated ABA signaling (Vilela et al 2015a). It is thus controversial whether casein kinase II subunits (CK2s) positively or negatively regulate ABA signaling in plants.…”

Section: Introductionmentioning

confidence: 99%

Casein kinase 2 α and β subunits inversely modulate ABA signal output in Arabidopsis protoplasts

Nagatoshi

Fujita

2018

Planta

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Main conclusionOur transient gene expression analyses in Arabidopsis protoplasts support the view that CK2αs and CK2βs positively and negatively modulate ABRE-dependent gene expression, respectively.The phytohormone abscisic acid (ABA) regulates the expression of thousands of genes via ABA-responsive elements (ABREs), and has a crucial role in abiotic stress response. Casein kinase II (CK2), a conserved Ser/Thr protein kinase in eukaryotes, is essential for plant viability. Although the CK2 has been known as a tetrameric holoenzyme comprised of two catalytic α and two regulatory β subunits, each of the two types of subunits has been proposed to have independent functions. The Arabidopsis genome encodes four α subunits (CK2α1, CK2α2, CK2α3, CK2α4) and four β subunits (CK2β1, CK2β2, CK2β3, CK2β4). There is a growing body of evidence linking CK2 to ABA signaling and abiotic stress responses. However, the roles of each CK2 subunit in ABA signaling remain largely elusive. Using the transient expression system with the core ABA signaling components in Arabidopsis leaf mesophyll protoplasts, we show here that CK2α1 and CK2α2 (CK2α1/2) positively modulate ABRE-dependent gene expression as ABA signal output in ABA signaling, whereas all four CK2βs negatively modulate the ABRE-dependent gene expression mediated by subclass III SnRK2–AREB/ABF pathway and by CK2α1/2. These data indicate that CK2α1/2 and CK2βs positively and negatively modulate ABA signal output, respectively, suggesting that the quantitative balance of CK2 subunits determines the ABA signal output in plants. Given that CK2s act as pleiotropic enzymes involved in multiple developmental and stress–responsive processes, our findings suggest that CK2 subunits may be involved in integration and coordination of ABA-dependent and -independent signaling.Electronic supplementary materialThe online version of this article (10.1007/s00425-018-2919-5) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Casein kinase 2 α and β subunits inversely modulate ABA signal output in Arabidopsis protoplasts

Nagatoshi

Fujita

2018

Planta

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“…Gibberellin and ABA are known to antagonistically regulate various developmental processes throughout the plant life cycle, including stress responses ( Yuan et al, 2017 ), seed germination and seedling growth ( Lin et al, 2015 ; Zhong et al, 2015 ; Liu et al, 2016 ), and the formation of arbuscular mycorrhizal symbiosis ( Martín-Rodríguez et al, 2016 ). For example, in rose ( R. hybrida ), the activity of Rh-PIP2;1 , which is involved in ethylene-mediated rose petal expansion, was reported to be enhanced by GA 3 at the later stages but suppressed by ABA during the early stages ( Ma et al, 2008 ; Li et al, 2009 ; Pei et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

A Mini Zinc-Finger Protein (MIF) from Gerbera hybrida Activates the GASA Protein Family Gene, GEG, to Inhibit Ray Petal Elongation

et al. 2017

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Petal appearance is an important horticultural trail that is generally used to evaluate the ornamental value of plants. However, knowledge of the molecular regulation of petal growth is mostly derived from analyses of Arabidopsis thaliana, and relatively little is known about this process in ornamental plants. Previously, GEG (Gerbera hybrida homolog of the gibberellin [GA]–stimulated transcript 1 [GAST1] from tomato), a gene from the GA stimulated Arabidopsis (GASA) family, was reported to be an inhibitor of ray petal growth in the ornamental species, G. hybrida. To explore the molecular regulatory mechanism of GEG in petal growth inhibition, a mini zinc-finger protein (MIF) was identified using yeast one-hybrid (Y1H) screen. The direct binding of GhMIF to the GEG promoter was verified by using an electrophoretic mobility shift assay and a dual-luciferase assay. A yeast two-hybrid (Y2H) revealed that GhMIF acts as a transcriptional activator. Transient transformation assay indicated that GhMIF is involved in inhibiting ray petal elongation by activating the expression of GEG. Spatiotemporal expression analyses and hormone treatment assay showed that the expression of GhMIF and GEG is coordinated during petal development. Taken together, these results suggest that GhMIF acts as a direct transcriptional activator of GEG, a gene from the GASA protein family to regulate the petal elongation.

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“…Expression of CKB1 under various stresses was analysed in our previous study (Yuan et al . ), which showed that exogenous ABA induced a rapid increase of CKB1 expression in 1 h. However, exogenous GA induced a gradual increase of CKB 1 over 8 h. For response to cold stress, expression of CKB1 was reduced under low temperatures. For response to osmotic stress, the expression of CKB1 first decreased and then increased with time when treated with mannitol.…”

Section: Discussionmentioning

confidence: 99%

“…The CKB1 gene over‐expression lines (CKB1‐OE) were generated by our lab and were described in a previous study (Yuan et al . ). Briefly, cDNA of Col‐0 was used as template to amplify fragments of the CKB1 gene with PCR.…”

Section: Methodsmentioning

confidence: 97%

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CKB1 regulates expression of ribosomal protein L10 family gene and plays a role in UV‐B response

Zhang

Yuan

et al. 2019

Plant Biol J

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Plastid casein kinase 2 (CK2), which is a major Ser/Thr-specific enzyme in higher organisms, plays an essential role in plant development and diverse abiotic stresses. CKB1 is a regulatory subunit beta of CK2. To expand our understand of functions of the CKB1 gene in Arabidopsis thaliana, protein changes among wild-type (WT) and CKB1 gain-and loss-of-function mutants were compared.• Proteins extracted from the CKB1 knockout mutant and overexpressing mutant were compared with Col-0 plants using 2D-PAGE. Proteins regulated by CKB1 were identified with matrix-assisted laser desorption ionisation time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF), and its transcript was verified by qRT-PCR. Bioinformatics analysis, including gene ontology and protein-protein interaction analysis, were employed.• The results of mass spectra and bioinformatics analysis suggest that CKB1 may have functions in regulation of the ribosomal protein L10 (RPL10) family and is involved in ultraviolet-B (UV-B) response. Furthermore, qRT-PCR verification showed CKB1 expression was up-regulated by UV-B stress. The expression levels of five genes in the RPL10 family were reduced in the ckb1 T-DNA insertion mutants, whereas they increased in the CKB1 overexpressing mutants under both normal conditions and UV-B treatment.• In conclusion, CKB1 has important functions in UV-B radiation stress. Our study implies that CKB1 positively regulates UV-B radiation stress signalling, possibly through modulating expression of the RPL10 family.

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CKB1 is involved in abscisic acid and gibberellic acid signaling to regulate stress responses in Arabidopsis thaliana

Cited by 16 publications

References 64 publications

Casein kinase 2 α and β subunits inversely modulate ABA signal output in Arabidopsis protoplasts

Casein kinase 2 α and β subunits inversely modulate ABA signal output in Arabidopsis protoplasts

A Mini Zinc-Finger Protein (MIF) from Gerbera hybrida Activates the GASA Protein Family Gene, GEG, to Inhibit Ray Petal Elongation

CKB1 regulates expression of ribosomal protein L10 family gene and plays a role in UV‐B response

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