Civa II Klorürün Sıçan Beyin Dokusunda Oluşturduğu Toksisiteye Karşı Borik Asidin Koruyucu Etkisinin İncelenmesi

Abstract

In this study, the protective effects of boric acid (BA) in the prevention of brain damage caused by mercury II chloride ( HgCl2) in rats were investigated. In the experiment, 24 adult and Wistar albino male rats weighing roundly 200-300 g were used. Group I (Control, n=8): Isotonic saline (i.p), Group II (HgCl2,n=8):(0.01g/kg)(oral), Group III (HgCl2 + (BA) (n:8): HgCl2(0.01g/kg) + BA (3.25mg/kg/day i.p.) group were administered. The rats in all groups were sacrificed at the end of the 10th day and their brain tissues were taken. Biochemical parameters including the enzyme activities of SOD, CAT and GSH-Px were measured. The enzyme activity of SOD was reduced in HgCl2 treated group in comparison to the control group (p<0.001). Activity of the enzyme was increased after BA administration (p˂0.001). CAT enzyme activity decreased in HgCl2 and HgCl2+BA administered groups with control (p˂0.001). An increase in enzyme activity in BA group with HgCl2 administered group was observed. GSH-Px enzyme activity decreased in HgCl2 (p˂0.001) and HgCl2+BA (p˂0.05) groups with control. However, an increase was found in BA administered group with HgCl2 administered group (p˂0.001). It is thought that antioxidant enzyme activities such as SOD, CAT and GSH-Px are significantly changed and BA may have a protective effect in the histopathological examination of brain tissue.