CITRX thioredoxin interacts with the tomato Cf-9 resistance protein and negatively regulates defence

Rivas, Susana; Rougon‐Cardoso, Alejandra; Smoker, Matthew; Schauser, Leif; Yoshioka, Hirofumi; Jones, Jonathan D. G.

doi:10.1038/sj.emboj.7600224

Cited by 125 publications

(117 citation statements)

References 55 publications

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“…Ascorbate-deficient mutants exhibit microlesions and constitutive PR gene expression, lack induction of H 2 O 2 -sensitive genes, and display higher tolerance to infection by P. syringae when compared to wild type (Pavet et al, 2005). Silencing of the tobacco thioredoxin CITRX resulted in enhanced pathogen-induced ROS accumulation, calcium-dependent protein kinase activation, and defense gene expression (Rivas et al, 2004). Moreover, there is a possible implication of the cytosolic thioredoxin TRXh5 in response to pathogens and to oxidative stresses (Laloi et al, 2004).…”

Section: Mapks: the Chicken Or The Egg In Ros Signaling?mentioning

confidence: 99%

Mitogen-Activated Protein Kinases and Reactive Oxygen Species Signaling in Plants

2006

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Section: Mapks: the Chicken Or The Egg In Ros Signaling?mentioning

confidence: 99%

Mitogen-Activated Protein Kinases and Reactive Oxygen Species Signaling in Plants

2006

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“…Hydrogen peroxide accumulation was detected by endogenous peroxidase-dependent in situ histochemical staining using 3,3Ј-diaminobenzidine as described in ref. 24.…”

Section: Agrobacterium-mediated Transient Expression In Plant Leavesmentioning

confidence: 99%

Ethylene-mediated cross-talk between calcium-dependent protein kinase and MAPK signaling controls stress responses in plants

Ludwig

Saitoh

Felix

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

292

204

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Plants are constantly exposed to environmental changes and need to integrate multiple external stress cues. Calcium-dependent protein kinases (CDPKs) are implicated as major primary Ca 2؉ sensors in plants. CDPK activation, like activation of mitogen-activated protein kinases (MAPKs), is triggered by biotic and abiotic stresses, although distinct stimulus-specific stress responses are induced. To investigate whether CDPKs are part of an underlying mechanism to guarantee response specificity, we identified CDPK-controlled signaling pathways. A truncated form of Nicotiana tabacum CDPK2 lacking its regulatory autoinhibitor and calcium-binding domains was ectopically expressed in Nicotiana benthamiana. Infiltrated leaves responded to an abiotic stress stimulus with the activation of biotic stress reactions. These responses included synthesis of reactive oxygen species, defense gene induction, and SGT1-dependent cell death. Furthermore, N-terminal CDPK2 signaling triggered enhanced levels of the phytohormones jasmonic acid, 12-oxo-phytodienoic acid, and ethylene but not salicylic acid. These responses, commonly only observed after challenge with a strong biotic stimulus, were prevented when the CDPK's intrinsic autoinhibitory peptide was coexpressed. Remarkably, elevated CDPK signaling compromised stress-induced MAPK activation, and this inhibition required ethylene synthesis and perception. These data indicate that CDPK and MAPK pathways do not function independently and that a concerted activation of both pathways controls response specificity to biotic and abiotic stress.

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“…In one report, virus-induced gene silencing of the cytosolic TRX-like protein CITRX convincingly demonstrated its implication in the Cf9/Avr9-triggered hypersensitive response (Rivas et al, 2004). The alternative NADPH-dependent redox pathway, which implicates glutaredoxins, is a potential source of redundancy.…”

Section: Introductionmentioning

confidence: 99%

Inactivation of Thioredoxin Reductases Reveals a Complex Interplay between Thioredoxin and Glutathione Pathways in Arabidopsis Development

et al. 2007

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NADPH-dependent thioredoxin reductases (NTRs) are key regulatory enzymes determining the redox state of the thioredoxin system. The Arabidopsis thaliana genome has two genes coding for NTRs (NTRA and NTRB), both of which encode mitochondrial and cytosolic isoforms. Surprisingly, plants of the ntra ntrb knockout mutant are viable and fertile, although with a wrinkled seed phenotype, slower plant growth, and pollen with reduced fitness. Thus, in contrast with mammals, our data demonstrate that neither cytosolic nor mitochondrial NTRs are essential in plants. Nevertheless, in the double mutant, the cytosolic thioredoxin h3 is only partially oxidized, suggesting an alternative mechanism for thioredoxin reduction. Plant growth in ntra ntrb plants is hypersensitive to buthionine sulfoximine (BSO), a specific inhibitor of glutathione biosynthesis, and thioredoxin h3 is totally oxidized under this treatment. Interestingly, this BSO-mediated growth arrest is fully reversible, suggesting that BSO induces a growth arrest signal but not a toxic accumulation of activated oxygen species. Moreover, crossing ntra ntrb with rootmeristemless1, a mutant blocked in root growth due to strongly reduced glutathione synthesis, led to complete inhibition of both shoot and root growth, indicating that either the NTR or the glutathione pathway is required for postembryonic activity in the apical meristem.

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CITRX thioredoxin interacts with the tomato Cf-9 resistance protein and negatively regulates defence

Cited by 125 publications

References 55 publications

Mitogen-Activated Protein Kinases and Reactive Oxygen Species Signaling in Plants

Mitogen-Activated Protein Kinases and Reactive Oxygen Species Signaling in Plants

Ethylene-mediated cross-talk between calcium-dependent protein kinase and MAPK signaling controls stress responses in plants

Inactivation of Thioredoxin Reductases Reveals a Complex Interplay between Thioredoxin and Glutathione Pathways in Arabidopsis Development

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