Citrus fruit flavonoids influence on neutrophil apoptosis and oxidative metabolism

Abstract

Citrus flavonoids are dietary antioxidants that may protect against oxidative stress linked to inflammation and reduce the risk of macromolecule damage caused by free radicals. Three citrus flavonoids - naringin, naringenin and hesperidin - have been examined for their ability to activate caspase-3, a marker of apoptosis execution, in human polymorphonuclear neutrophils in vitro, stimulated and non-stimulated with phorbol 12-myristate 13-acetate. The flavonoid potency to reduce reactive oxygen species generation was also assessed in measurements of superoxide radical and luminol dependent chemiluminescence. The caspase-3 activity depended on the time of flavonoid treatment and was increased markedly in phorbol-treated cells incubated with flavonoids for 24 h. Hesperidin at a concentration of 100 microm exerted the strongest stimulating effect on the enzyme (137% of control). Flavonoids inhibited the neutrophil ability to generate superoxide radical and 10-100 microM hesperidin appeared the most active phytochemical. The antioxidant capacity of the examined flavonoids was most prominently expressed in the chemiluminescent measurements. The obtained results suggest that reactive oxygen species may inhibit apoptosis via caspase-3 inhibition and the antioxidant action of citrus flavonoids may reverse this process.

Evaluation of antioxidative and anti-inflammatory potential of hesperidin and naringin on the rat air pouch model of inflammation

“…These results suggest the antiperoxidative potential of naringin, which has been reported earlier [5].…”

Antioxidant and cytotoxic properties of an aqueous extract from the Argentinean plantHedeoma multiflorum

“…A reduction in neutrophils apoptosis has already been linked to several inflammatory conditions, including rheumatoid arthritis and acute respiratory distress syndrome (Chilvers et al, 2000), and there is some evidence that pharmacological manipulation can reduce or increase the inflammatory cell apoptosis (Ward et al, 1999). Recently, it was also reported that some antioxidant may revert the inhibition of human PMN neutrophil apoptosis, via caspas-3, a principle apoptosis executor, induced by reactive oxygen species (Zielinska-Przyjemska & Ignatowicz, 2008). Previously, we have shown that caffeoyl conjugates exert a marked notable activity on some of the biological functions of PMN implicated in the initiation and maintenance of inflammation (Góngora et al, 2002).…”

Protective effects of neohesperidin and poncirin isolated from the fruits of Poncirus trifoliata on potential gastric disease

“…The formation zymosan-stimulated human neutrophils in inflammation (Zielińska-Przyjemska and Ignatowicz, 2008). As shown in Table 1, neohesperidin, which is a derivative of hesperidin, with free radical scavenging activity is expected to protect against gastric mucosal injury through the antioxidative mechanism.…”