Citrus flavonoids repress the mRNA for stearoyl-CoA desaturase, a key enzyme in lipid synthesis and obesity control, in rat primary hepatocytes

Nichols, LaNita A.; Jackson, Daniel M.; Manthey, John A.; Shukla, Shivendra D.; Holland, Lené J.

doi:10.1186/1476-511x-10-36

Cited by 43 publications

(27 citation statements)

References 35 publications

(38 reference statements)

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“…Our hypothesis is supported by studies showing that hesperidin could inhibit both hepatic HMG-CoA reductase and ACAT activities, while increasing fecal cholesterol and LDL receptor mRNA levels in animals and cells (56 -59). Furthermore, it also suppressed the mRNA expression for stearoyl-CoA desaturase, a key enzyme in lipid synthesis in rat primary hepatocytes (60).…”

Section: Discussionmentioning

confidence: 97%

Effects of Hesperidin on the Progression of Hypercholesterolemia and Fatty Liver Induced by High-Cholesterol Diet in Rats

et al. 2011

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Abstract. The protective effects of hesperidin against hypercholesterolemia and fatty liver were examined in male Wistar rats fed a high-cholesterol diet for 12 weeks. Compared with a standard diet, a high-cholesterol diet not only increased body weights, liver weights, and serum concentration of cholesterol, but also induced the fatty degeneration (steatosis) of liver. Hesperidin (0.08%) reduced levels of hepatic steatosis, adipose tissue and liver weights (P < 0.05), serum total cholesterol and retinol binding protein (RBP) 4 concentrations (P < 0.05) in rats fed with high-cholesterol diet, while reduction in low-density lipoprotein cholesterol levels and triglyceride concentrations was not significant. It also attenuated the marked changes in mRNA expression of lipid metabolism-related proteins: RBP, heart fatty acid-binding protein (H-FABP), and cutaneous fatty acidbinding protein (C-FABP), in liver and adipose tissue. According to the results of gas chromatography, serum concentrations of total cholesterol and biomarkers of cholesterol synthesis (lathosterol) and absorption (campesterol, β-sitosterol) were lower, and concentrations of cholesterol in feces were higher in the rats given hesperidin (P < 0.05). Hesperidin may improve hypercholesterolemia and fatty liver by inhibiting both the synthesis and absorption of cholesterol and regulating the expression of mRNA for RBP, C-FABP, and H-FABP.

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Section: Discussionmentioning

confidence: 97%

Effects of Hesperidin on the Progression of Hypercholesterolemia and Fatty Liver Induced by High-Cholesterol Diet in Rats

et al. 2011

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“…It is reported that many flavonoids have lipid-lowering effects [14,15]. Luteolin has been reported to reduce the accumulation of lipids in HepG2 cells [16].…”

Section: Discussionmentioning

confidence: 99%

Bioactivity-guided fractionation of the triglyceride-lowering component and in vivo and in vitro evaluation of hypolipidemic effects of Calyx seu Fructus Physalis

et al. 2012

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BackgroundIn folklore, some people take the decoction of Calyx seu Fructus Physalis (CSFP) for lowering blood lipids. The present study is designed to evaluate the lipid-lowering activities of CSFP, and search for its pharmacodynamical material.MethodsCSFP was extracted by water and 75% ethanol, respectively. The extracts of CSFP for reducing serum lipid levels were evaluated on mouse model of hyperlipidemia. The optimized extract was subjected to the bioactivity-guided fractionation in which the liquid-liquid extraction, collumn chromatography, the in vivo and in vitro models of hyperlipidemia were utilized. The structure of active component was determined by 13 C-NMR and 1H-NMR.ResultsThe 75% ethanol extract of CSFP decreased the serum total cholesterol (TC) and triglyceride (TG) levels in mouse model of hyperlipidemia. Followed a separation process for the 75% ethanol extract of CSFP, the fraction B was proved to be an active fraction for lowering lipid in vivo and in vitro experiments, which could significantly decrease the serum TC and TG levels in mouse model of hyperlipidemia, and remarkably decrease the increase of TG in primary mouse hepatocytes induced by high glucose and the increase of TG in HepG2 cells induced by oleic acid. The fraction B2, isolated from B on bioactivity-guided fractionation, could significantly decrease TG level in HepG2 cells. One compound with the highest content in B2 was isolated and determined as luteolin-7-O-beta-D-glucopyranoside by NMR spectra. It could significantly reduce the TG level in HepG2 cells, and inhibited the accumulation of lipids by oil red O stain.ConclusionOur results demonstrated that the 75% ethanol extract of CSFP could improve in vitro and in vivo lipid accumulation. Luteolin-7-O-beta-D-glucopyranoside might be a leading pharmacodynamical material of CSFP for lowering lipids.

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“…A significant increase of lipoprotein lipase and lecithin acyltransferase (LCAT) on feeding Ficus bengalensis flavonoids and quercetin to such groups was seen by Daniel et al [32]. Work done by Nichols et al [33] showed that citrus flavonoids regulated the transcription of the lowdensity lipoprotein receptor (LDLR) gene in HepG2 cells leading to their hypo-cholesterolemic effects.…”

Section: Discussionmentioning

confidence: 99%

A Study of the Hypolipidemic and Antioxidant Activities of Whole Plant Extracts of Ipomoea Aquatica Fork in Experimentally Induced Hyperlipidemia in Rabbits

Aziz

Gohain

2016

Int J Pharm Pharm Sci

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Objective: The aim of the study has been to investigate the possible hypolipidemic and antioxidant properties of the whole plant extract of Ipomoea aquatica in experimentally induced hyperlipidemia in rabbits.Methods: Ethanolic extract of I. aquatica whole plant (EEIAWP) was prepared by percolation method. The extract was evaluated for hypolipidemic and antioxidant activities using 400 mg/kg body weight per day in a high fat diet induced hyperlipidemia in rabbits. The results were analyzed using one-way analysis of variance (ANOVA) followed by Bonferroni's multiple comparison tests and compared to the normal control, experimental control and the standard drug (atorvastatin 2.1 mg/kg body weight per day) groups. The results were expressed as mean±standard error of mean (SEM). Values with p<0.05 were considered significant. Results:Oral administration of EEIAWP in the test group showed a significant reduction in the serum levels of total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C) and a significant increase in the high-density lipoprotein cholesterol (HDL-C) when compared to the experimental control group. There were also significantly elevated catalase and superoxide dismutase (SOD) activities and significantly lower malondialdehyde (MDA) levels in the test group compared to the experimental group. Similar results were also found in the standard drug group. Conclusion:The results of our experiment demonstrated that EEIAWP possesses significant antihyperlipidemic and antioxidant activities and hence could be a potential source of medication as an adjuvant to the existing therapy for treatment of dyslipidemia.

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Citrus flavonoids repress the mRNA for stearoyl-CoA desaturase, a key enzyme in lipid synthesis and obesity control, in rat primary hepatocytes

Cited by 43 publications

References 35 publications

Effects of Hesperidin on the Progression of Hypercholesterolemia and Fatty Liver Induced by High-Cholesterol Diet in Rats

Effects of Hesperidin on the Progression of Hypercholesterolemia and Fatty Liver Induced by High-Cholesterol Diet in Rats

Bioactivity-guided fractionation of the triglyceride-lowering component and in vivo and in vitro evaluation of hypolipidemic effects of Calyx seu Fructus Physalis

A Study of the Hypolipidemic and Antioxidant Activities of Whole Plant Extracts of Ipomoea Aquatica Fork in Experimentally Induced Hyperlipidemia in Rabbits

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