Citrus Extracts as Inhibitors of Quorum Sensing, Biofilm Formation and Motility of<i>Campylobacter jejuni</i>

Castillo, Sandra; Heredia, Norma; Aréchiga-Carvajal, Elva Teresa; García, Santos

doi:10.1080/08905436.2014.895947

Cited by 52 publications

(49 citation statements)

References 37 publications

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“…Our results from the cell culture revealed that BR reduced C. jejuni adhesion and invasion of human enterocytes by 0.7 and 0.5 Log CFU/ml respectively compared to control. This reduction was similar to that observed with other phytochemicals such as berry extracts (Salaheen et al, 2014), and extracts from Acacia farnesiana , Artemisia ludoviciana , Opuntia ficus-indica , and Cynara scolymus (Castillo et al, 2014). Since Campylobacter adhesion to epithelial cells is an important step for colonization (Jin et al, 2001; Hermans et al, 2011a), a reduction in this virulence attribute could potentially reduce colonization in birds.…”

Section: Discussionsupporting

confidence: 84%

“…We found that presence of BR in the medium reduced Campylobacter motility as compared to controls. Similar results of reduced Campylobacter motility were observed when Campylobacter was exposed to citrus extract (Castillo et al, 2014) and berries (Salaheen et al, 2014). Since a chicken cecal epithelial cell line is not commercially available, we used the well-established human epithelial cells (Caco-2) for conducting attachment and invasion assays.…”

Section: Discussionsupporting

confidence: 78%

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Application of β-Resorcylic Acid as Potential Antimicrobial Feed Additive to Reduce Campylobacter Colonization in Broiler Chickens

et al. 2017

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Campylobacter is one of the major foodborne pathogens that result in severe gastroenteritis in humans, primarily through consumption of contaminated poultry products. Chickens are the reservoir host of Campylobacter, where the pathogen colonizes the ceca, thereby leading to contamination of carcass during slaughter. A reduction in cecal colonization by Campylobacter would directly translate into reduced product contamination and risk of human infections. With increasing consumer demand for antibiotic free chickens, significant research is being conducted to discover natural, safe and economical antimicrobials that can effectively control Campylobacter colonization in birds. This study investigated the efficacy of in-feed supplementation of a phytophenolic compound, β-resorcylic acid (BR) for reducing Campylobacter colonization in broiler chickens. In two separate, replicate trials, day-old-chicks (Cobb500; n = 10 birds/treatment) were fed with BR (0, 0.25, 0.5, or 1%) in feed for a period of 14 days (n = 40/trial). Birds were challenged with a four-strain mixture of Campylobacter jejuni (∼106 CFU/ml; 250 μl/bird) on day 7 and cecal samples were collected on day 14 for enumerating surviving Campylobacter in cecal contents. In addition, the effect of BR on the critical colonization factors of Campylobacter (motility, epithelial cell attachment) was studied using phenotypic assay, cell culture, and real-time quantitative PCR. Supplementation of BR in poultry feed for 14 days at 0.5 and 1% reduced Campylobacter populations in cecal contents by ∼2.5 and 1.7 Log CFU/g, respectively (P < 0.05). No significant differences in feed intake and body weight gain were observed between control and treatment birds fed the compound (P > 0.05). Follow up mechanistic analysis revealed that sub-inhibitory concentration of BR significantly reduced Campylobacter motility, attachment to and invasion of Caco-2 cells. In addition, the expression of C. jejuni genes coding for motility (motA, motB, fliA) and attachment (jlpA, ciaB) was down-regulated as compared to controls (P < 0.05). These results suggest that BR could potentially be used as a feed additive to reduce Campylobacter colonization in broilers.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionsupporting

confidence: 78%

Application of β-Resorcylic Acid as Potential Antimicrobial Feed Additive to Reduce Campylobacter Colonization in Broiler Chickens

et al. 2017

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“…The polyphenolic extract of bergamot peel, although less effective, The data acquired showed that the polyphenolic extracts obtained from the two portions (peel and pulp) of the three Citrus taxa were generally able not only to limit the formation of biofilm by pathogenic bacteria, but also to alter the metabolic activity of the cells present within the biofilm, thus counteracting the formation of biofilm on several fronts, including that of acting on the metabolism of biofilm bacterial cells. This confirms the great and interesting applicative versatility of Citrus, whose species can provide extracts or essential oils capable of preventing or at least reducing, the pathogenicity of different bacteria, acting through different mechanisms of action [38]. The presence of flavonoids in the extracts appears to modulate the mechanisms of cellular communication.…”

Section: Biofilm Formation and Metabolic Activity Of Biofilm Cellssupporting

confidence: 61%

Polyphenols, Antioxidant, Antibacterial, and Biofilm Inhibitory Activities of Peel and Pulp of Citrus medica L., Citrus bergamia, and Citrus medica cv. Salò Cultivated in Southern Italy

et al. 2019

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The aim of this paper was to study the polyphenols of peel and pulp of three Citrus taxa-Citrus medica, Citrus bergamia, and Citrus medica cv. Salò-cultivated in the Cosenza province, Southern Italy, and to evaluate their antioxidant and antibacterial activity, performed against Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Staphylococcus aureus, and Pectobacterium carotovorum. Furthermore, we assessed the inhibitory effect of the extracts on bacterial capacity to form biofilm, and on the metabolic activity of the cells present therein. The results indicated that such extracts could find new potential applications in the field of natural antioxidant and anti-bacterial agents in pharmaceutics, agriculture, and food fields.

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“…Castillo et al [25] reported that citrus extracts reduced C. jejuni AI-2 activity in this bioluminescence assay, which correlated with its motility and biofilm formation. Furthermore, these citrus extracts reduced C. jejuni invasion of and adhesion to HeLa cells and the expression of the virulence genes cadF and ciaB [26].…”

Section: Discussionmentioning

confidence: 64%

Modulation of Campylobacter jejuni Motility, Adhesion to Polystyrene Surfaces, and Invasion of INT407 Cells by Quorum-Sensing Inhibition

Šimunović

Ramić

et al. 2020

Microorganisms

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Campylobacter jejuni is a major foodborne pathogen, and the LuxS-mediated quorum-sensing (QS) system influences its motility, biofilm formation, invasion, host colonization, and virulence. QS therefore represents a target for the control of C. jejuni. The aim of this study was to investigate the correlation of QS inhibition with changes in C. jejuni motility, adhesion to polystyrene surfaces, and adhesion to and invasion of INT407 cells. This was achieved by studying (i) the luxS-deficient mutant and (ii) treatment of C. jejuni with 20 natural extracts as six essential oils, 11 ethanolic extracts, and three pure compounds. Compared to the wild-type, the ΔluxS mutant showed decreased motility, adhesion to polystyrene surfaces, and invasion of INT407 cells. The anti-QS effects of the treatments (n = 15/20) were assayed using Vibrio harveyi BB170 bioluminescence. Moderate positive correlation was shown between C. jejuni QS reduction and reduced motility (τ = 0.492, p = 0.024), adhesion to polystyrene surfaces (τ = 0.419, p = 0.008), and invasion (r = 0.394, p = 0.068). The best overall effect was achieved with a Sedum rosea (roseroot) extract, with 96% QS reduction, a 1.41 log (96%) decrease in adhesion to polystyrene surfaces, and an 82% decrease in invasion. We show that natural extracts can reduce motility, adhesion to polystyrene surfaces, and invasion of INT407 cells by C. jejuni through modulation of the LuxS (QS) system.

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Citrus Extracts as Inhibitors of Quorum Sensing, Biofilm Formation and Motility ofCampylobacter jejuni

Cited by 52 publications

References 37 publications

Application of β-Resorcylic Acid as Potential Antimicrobial Feed Additive to Reduce Campylobacter Colonization in Broiler Chickens

Application of β-Resorcylic Acid as Potential Antimicrobial Feed Additive to Reduce Campylobacter Colonization in Broiler Chickens

Polyphenols, Antioxidant, Antibacterial, and Biofilm Inhibitory Activities of Peel and Pulp of Citrus medica L., Citrus bergamia, and Citrus medica cv. Salò Cultivated in Southern Italy

Modulation of Campylobacter jejuni Motility, Adhesion to Polystyrene Surfaces, and Invasion of INT407 Cells by Quorum-Sensing Inhibition

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