Citrullus colocynthis (L.) Schrad: Chemical characterization, scavenging and cytotoxic activities

Bourhia, Mohammed; Messaoudi, Mouhcine; Bakrim, Hanane; Mothana, Ramzi A.; Sddiqui, Nasir A.; Almarfadi, Omer M.; Mzibri, Mohammed El; Ghalem, Saïd; Laglaoui, Amin; Benbacer, Laïla

doi:10.1515/chem-2020-0124

Cited by 25 publications

(19 citation statements)

References 19 publications

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“…The pharmacological activities investigated in this study are frequently related to the identified compounds in the C. colocynthis seed extract since the chemical analysis showed many potentially bioactive compounds with pharmacological activities, including antioxidant and antibacterial activities as reported in earlier works [30][31][32][33][34][35]. In this sense, Jasmonic acid played a crucial role in defense against insects, pathogenic microorganisms, kin aging, as well cancer development [36,37].…”

Section: Discussionmentioning

confidence: 54%

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Chemical Profiling, Antioxidant, Antiproliferative, and Antibacterial Potentials of Chemically Characterized Extract of Citrullus colocynthis L. Seeds

Bourhia

Bouothmany

Bakrim³

et al. 2021

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Background: Citrullus colocynthis L. (C. colocynthis) is commonly known as colocynth. It belongs to the family Cucurbitaceae that is frequently used in alternative medicine in the north of Africa. The aim of the study: the present research was undertaken to investigate the chemical composition, antioxidant, antiproliferative, and antibacterial potentials of C. colocynthis seed extract. Material and methods: the chemical composition of C. colocynthis seed organic extract was characterized using gas chromatography/mass spectrometry (GC-MS). The antioxidant property was carried out using both β-carotene bleaching and DPPH assays. The antibacterial effect was effectuated using the agar disc diffusion method. The antiproliferative activity vs. human colorectal adenocarcinoma cell line (HT-29) and human breast adenocarcinoma cell line (MDA MB 231) were carried by WST-1 test. The chemical analysis showed the presence of interesting potentially bioactive compounds. The studied plant extract exhibited antioxidant potential with IC50 value of 2. 22 mg/mL (β-carotene bleaching) and 8.98 ± 0.619 mg/mL (DPPH). Concerning the antiproliferative activity, the seed extract was effective in MDA-MB-231 and HT-29 cancer cells with IC50 values 86.89 ± 3.395 and 242.1 ± 17.9 μg/mL, respectively, whilst the extract of Citrullus colocynthis seeds was non-toxic in healthy human dermal fibroblasts. Regarding the antibacterial test, the extract was effective in Gram-positive bacteria only. Conclusion: The outcome of this research indicated that the extracts from C. colocynthis seeds may compose a promising source with interesting compounds that can be used to fight cancer, free radicals damage, and bacterial infections.

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Section: Discussionmentioning

confidence: 54%

“…In this sense, the differences can be attributed to solvent polarities used for extraction. The antibacterial activity could probably due to the presence of cucurbitacin molecules since the cucurbitacin E was effective against M. tuberculosis H37Rvat [32].…”

Section: Antibacterial Activitymentioning

confidence: 99%

Chemical Profiling, Antioxidant, Antiproliferative, and Antibacterial Potentials of Chemically Characterized Extract of Citrullus colocynthis L. Seeds

Bourhia

Bouothmany

Bakrim³

et al. 2021

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“…Using CC fruits and seeds extracts was reported to alleviate the oxidative stress by increasing SOD and GHS-Px activities, while decreasing the MDA levels in the induced-Parkinson'sdiseased mouse model [46]. The presented positive impact of CCs supplementation on redox status to heat-stressed broilers can be contributed to its antioxidant compound contents (e.g., α-tocopherol, γ-tocopherol, β-carotene, phenolic acid and flavonoids) [18,42,47,48].…”

Section: Discussionmentioning

confidence: 99%

Citrullus colocynthis Seeds: A Potential Natural Immune Modulator Source for Broiler Reared under Chronic Heat Stress

Alzarah

Althobiati

Abbas

et al. 2021

Animals

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There is an extensive search for natural products that can be introduced to broiler rations to improve performance, especially during the unfavorable breeding conditions. Under heat-stress conditions, the immune response seriously deteriorates, which consequently impairs broiler production performance. Thus, the present study aimed to investigate the potentials of Citrullus colocynthis seeds (CCs) supplementation to modulate the immune response of broilers subjected to chronic heat stress. A total of 300 Cobb-500 male broiler chickens aged 21 days were randomly divided into two equal groups and reared under either thermo-neutral condition (24 ± 1 °C) or subjected to cyclic heat stress (34 ± 1 °C for 8 h). Each group was further divided into two groups (5 replicate × 15 chicks) and was fed either the basal diet or the basal diet with 0.1% CCs supplementation. The results showed that heat stress impaired the production performance by lowering the final body weight and feed intake as well as impairing feed conversion. The levels of stress markers (i.e., malondialdehyde, tumor necrosis factor-α and corticosterone) increased (p < 0.05), whereas the activity of antioxidant enzymes decreased in broilers exposed to heat stress. Further, heat stress caused direct suppression of broiler humoral and cell-mediated immune responses. The stimulating index of T and B lymphocytes proliferation, as well as the antibody titer against sheep red blood cells, were significantly (p < 0.05) reduced by heat-stress exposure. However, CCs supplementation to broilers subjected to heat stress improved (p < 0.05) the final body weight, feed intake, and feed conversion ratio (FCR), compared to the non-supplemented stressed group. The cellular and cell-mediated immune response indicators significantly enhanced (p < 0.05) with CCs supplementation. Supplementation of CCs to broilers reared under similar environmental conditions elevated the total white blood cells (TWBCs) count and the broiler stimulating index of T and B lymphocytes. It can be concluded that CC seeds can be effectively used to stimulate the immune response and improve the production performance of broilers reared under heat-stress condition.

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“…Cucurbitacin B, E, and I, among the most potent components of C. colocynthis seed, have been shown to have antibacterial action against Staphylococcus aureus, Bacillus cereus, and K. pneumonia (Ali et al 2013). Cucurbitacin E has also been shown to have antibacterial properties against M. tuberculosis H37Rvat (Bourhia et al 2020). The biological activity of plant extracts is dependent on the solvent and extraction method used, and the antimicrobial effectiveness of plant extracts is dependent on the active substances, selected bacterial strains, and plant parts tested,so antimicrobial activity may differ from one bacterium gram negative to another gram positive (Qaralleh et al 2019).…”

Section: Discussionmentioning

confidence: 99%

Ruta Graveolens, Peganum Harmala and Citrullus Colocynthis Methanolic Extracts Have in Vitro Protoscolocidal Effects and Act Against Bacteria Isolated From Echinococcal Hydatid Cyst Fliud

Qaisi

Khleifat

Oran

et al. 2022

Preprint

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Echinococcosis is a common and endemic disease that affects both humans and animals. In this study, the in vitro activities of methanolic extracts of Ruta graveolens, Peganum harmala aerial parts, and Citrullus colocynthis seeds against protoscolosis and isolated bacterial strains from hydatid cysts were assessed using disc diffusion methos and Minimum Inhibitory Concentration (MIC). The chemical composition of aerial sections of R. graveolensand P. harmala, as well as methanolic extracts of C. colocynthis seeds, was studied using LC-MS. These plants produced a total of 26, 31, and 23 chemicals, respectively. The bacteria listed below were isolated from hydatid cyst fluid collected from a variety of sick locations, including the lung and liver. Micrococcus spp., E. coli, Klebsiella oxytoca, Enterobacter aerogenes, Enterobacter amnigenus, Pseudomonas aeruginosa, Staphylococcus xylosus, and Achromobacter xylosoxidans are among the bacteria that have been identified. The most effective extract was R. graveolens, followed by P. harmala and C. colocynthis, according to the results of antibacterial activity using the disc diffusion method. R. graveolens extract had the lowest MIC values (less than 2 mg/mL) against all microorganisms tested. This shows that the R. graveolens extract has additional properties, such as the ability to be both scolocidal and bactericidal. Because these bacteria are among the most prevalent pathogenic bacteria that increase the risk of secondary infection during hydatid cysts, the results of inhibitory zones and MICs of the R. graveolens methanol extract are considered highly promising.

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Citrullus colocynthis (L.) Schrad: Chemical characterization, scavenging and cytotoxic activities

Cited by 25 publications

References 19 publications

Chemical Profiling, Antioxidant, Antiproliferative, and Antibacterial Potentials of Chemically Characterized Extract of Citrullus colocynthis L. Seeds

Chemical Profiling, Antioxidant, Antiproliferative, and Antibacterial Potentials of Chemically Characterized Extract of Citrullus colocynthis L. Seeds

Citrullus colocynthis Seeds: A Potential Natural Immune Modulator Source for Broiler Reared under Chronic Heat Stress

Ruta Graveolens, Peganum Harmala and Citrullus Colocynthis Methanolic Extracts Have in Vitro Protoscolocidal Effects and Act Against Bacteria Isolated From Echinococcal Hydatid Cyst Fliud

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