Citron Kinase is an essential effector of the Pbl-activated Rho signalling pathway in<i>Drosophila melanogaster</i>

Shandala, Tetyana; Gregory, Stephen L.; Dalton, Hazel; Smallhorn, Masha; Saint, Robert

doi:10.1242/dev.01382

Cited by 47 publications

(61 citation statements)

References 63 publications

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“…This phenotype was similar at temperatures ranging from 18°to 36°b ut was more dramatic on solid media (where many cells had up to seven or nine septa) than in liquid media. In a typical time-lapse series ( Figure 5A; File S3), no successful cell separation was observed during .4.5 hr , as well as a Rho-GEF from Drosophila (Pebble) and one from humans (ECT2) that have also been implicated in cytokinesis (Miki et al 1993;Prokopenko et al 1999;Tatsumoto et al 1999;O'Keefe et al 2001;Giansanti et al 2004;Shandala et al 2004). Clustal W was used to align the Rho-GEF domains and then derive a phylogenetic tree based on this alignment.…”

Section: Resultsmentioning

confidence: 99%

Cooperation Between the Septins and the Actomyosin Ring and Role of a Cell-Integrity Pathway During Cell Division in Fission Yeast

Wang

et al. 2010

Genetics

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A major question about cytokinesis concerns the role of the septin proteins, which localize to the division site in all animal and fungal cells but are essential for cytokinesis only in some cell types. For example, in Schizosaccharomyces pombe, four septins localize to the division site, but deletion of the four genes produces only a modest delay in cell separation. To ask if the S. pombe septins function redundantly in cytokinesis, we conducted a synthetic-lethal screen in a septin-deficient strain and identified seven mutations. One mutation affects Cdc4, a myosin light chain that is an essential component of the cytokinetic actomyosin ring. Five others cause frequent cell lysis during cell separation and map to two loci. These mutations and their dosage suppressors define a signaling pathway (including Rho1 and a novel arrestin) for repairing cell-wall damage. The seventh mutation affects the poorly understood RNAbinding protein Scw1 and severely delays cell separation when combined either with a septin mutation or with a mutation affecting the septin-interacting, anillin-like protein Mid2, suggesting that Scw1 functions in a pathway parallel to that of the septins. Taken together, our results suggest that the S. pombe septins participate redundantly in one or more pathways that cooperate with the actomyosin ring during cytokinesis and that a septin defect causes septum defects that can be repaired effectively only when the cellintegrity pathway is intact.

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Section: Resultsmentioning

confidence: 99%

Cooperation Between the Septins and the Actomyosin Ring and Role of a Cell-Integrity Pathway During Cell Division in Fission Yeast

Wang

et al. 2010

Genetics

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“…However, CIT-K-knockout mice display cytokinesis defects only in some cell populations of the central nervous system and during spermatogenesis, indicating that CIT-K may be a tissue-specific Rho effector during cytokinesis (Di Cunto et al, 2000;Di Cunto et al, 2002). By contrast, the Drosophila CIT-K orthologue, Sticky (STI), is essential for cell division in all tissues, and its inactivation by mutation or RNAi leads to late cytokinesis failure accompanied by the formation of abnormal actin blebs at the equatorial cortex (D' Avino et al, 2004;Echard et al, 2004;Naim et al, 2004;Shandala et al, 2004). CIT-K in flies might thus be important for contractile ring organization rather than contractility (D'Avino et al, 2004).…”

Section: Regulation Of Cortical Activity During Cytokinesismentioning

confidence: 99%

Cleavage furrow formation and ingression during animal cytokinesis: a microtubule legacy

D’Avino

Savoian

Glover

2005

Journal of Cell Science

171

163

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Cytokinesis ensures the proper partitioning of the nuclear and cytoplasmic contents into independent daughter cells at the end of cell division. Although the metazoan mitotic spindle has been implicated in the placement and advancement of the cleavage furrow, the molecules responsible for these processes have remained elusive. Recent studies have provided insights into the role of different microtubule structures and associated proteins in cleavage furrow positioning and ingression together with the signalling events that regulate the dynamics of the equatorial cell cortex during cytokinesis. We try to unify these findings into a general model of cytokinesis in which both astral and central spindle microtubules have the ability to induce furrowing. We further propose that the evolutionarily conserved centralspindlin complex serves as a master controller of cell cleavage in Drosophila by promoting both furrow formation and ingression. The same mechanism might be conserved in other organisms.

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“…In drosophila and HeLa cells, citron-K loss of function causes defects in intercellular bridge structure and stability, delays resolution, and causes reversion of cytokinesis and multinucleation, indicating a requirement for citron-K at the end of cytokinesis for cellular abscission (38,(43)(44)(45)(46). Both flies and mice lacking citron-K exhibit severe phenotypes in neuroblasts and spermatocytes due to cytokinesis defect and induction of apoptosis (43,(45)(46)(47)(48).…”

Section: Introductionmentioning

confidence: 99%

p27Kip1 controls cytokinesis via the regulation of citron kinase activation

Serres

Kossatz-Boehlert²,

Chi³

et al. 2012

J. Clin. Invest.

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p27 Kip1 (p27) acts as a tumor suppressor by inhibiting cyclin-cyclin-dependent kinase (cyclin-CDK) activity. However, mice expressing a form of p27 that is unable to bind or inhibit cyclin-CDK complexes (p27 CK-) have increased incidence of tumor development as compared with wild-type and p27 -/-mice, revealing an oncogenic role for p27. Here, we identified a phenotype of multinucleation and polyploidy in p27 CK-mice not present in p27 -/-animals, suggesting a role for p27 in G 2 /M that is independent of cyclin-CDK regulation. Further analysis revealed that p27 CK-expression caused a cytokinesis and abscission defect in mouse embryonic fibroblasts. We identified the Rho effector citron kinase (citron-K) as a p27-interacting protein in vitro and in vivo and found that p27 and citron-K colocalized at the contractile ring and mid-body during telophase and cytokinesis. Moreover, overexpression of the minimal p27-binding domain of citron-K was sufficient to rescue the phenotype caused by p27 CK-. Conversely, expression of a mutant p27 CK-unable to bind citron-K did not induce multinucleation. Finally, by binding to citron-K, p27 prevented the interaction of citron-K with its activator RhoA. Taken together, these data suggest a role for p27 during cytokinesis via the regulation of citron-K activity.

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Citron Kinase is an essential effector of the Pbl-activated Rho signalling pathway inDrosophila melanogaster

Cited by 47 publications

References 63 publications

Cooperation Between the Septins and the Actomyosin Ring and Role of a Cell-Integrity Pathway During Cell Division in Fission Yeast

Cooperation Between the Septins and the Actomyosin Ring and Role of a Cell-Integrity Pathway During Cell Division in Fission Yeast

Cleavage furrow formation and ingression during animal cytokinesis: a microtubule legacy

p27Kip1 controls cytokinesis via the regulation of citron kinase activation

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