Citrobacter freundii Mutants Deficient in Host Specificity Functions and Their Recipient Ability for Foreign Deoxyribonucleic Acid

Graaff, J. de; Stouthamer, A. H.

doi:10.1099/00221287-67-1-91

Cited by 16 publications

(9 citation statements)

References 32 publications

(14 reference statements)

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“…Mutants with increased recipient ability were isolated and, in most cases, proven to be restriction-deficient, by measuring the efficiencies of plating of suitable bacteriophages (Colson & Colson, 1967;Okada, Watanabe & Miyake, 1968;de Graaf & Stouthamer, 1971).…”

Section: G C O R N E L I S a N D C C O L S O N Discussionmentioning

confidence: 99%

Restriction of DNA in Yersinia enterocolitica Detected Recipient Ability for a Derepressed R Factor from Escherichia coli

Cornelis

Colson²

1975

Journal of General Microbiology

114

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A derepressed R factor, RYzdrdz, was transferred at a frequency of 5 x I O -~ between two strains of Yersinia enterocolitica mated on a membrane. Under the same conditions transfer of this R factor from Escherichia coli to Y. enterocolitica was observed at a frequency of only 7.7 x IO-~. This frequency was greatly increased when the recipient strain was heat-treated before mating. Heat exposure for optimum fertility was 50 to 52 "C for a period of 2 to 3 min. Mutants of Y. enterocolitica were isolated which were infected by RYzdrd;! from E. coli or from Y. enterocolitica at the same frequency. These observations strongly suggest that a DNA restriction and modification system in Y. enterocolitica causes its low recipient ability for plasmids from other species.

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Section: G C O R N E L I S a N D C C O L S O N Discussionmentioning

confidence: 99%

Restriction of DNA in Yersinia enterocolitica Detected Recipient Ability for a Derepressed R Factor from Escherichia coli

Cornelis

Colson²

1975

Journal of General Microbiology

114

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“…Citrobacter freundii strain ~~~~9 7 5 0 was maintained on minimal agar plus glucose (MAG) supplemented with either IOO /AM-methionine, or with 133 pM-methionhe, I mMsodium glutamate, I 10 pwlysine and 9 pM-thiamin (de Graaf & Stouthamer, 1971). Cultures were stored at 4 "C for up to 4 weeks.…”

Section: E T H O D Smentioning

confidence: 99%

“…In contrast, other species grow poorly, ifat al1,unless supplementsof amino acids and vitamins are added (Vkron & Le Minor, 1975). Citrobacter freundii is normally cultured in complex media, but aerobic growth also occurs in a minimal medium supplemented with glutamate, methionine, lysine and thiamin (de Graaf & Stouthamer, 1971). We used this simplified medium for our recent investigation into the regulation of aerobic and anaerobic metabolism in C. freundii, but found on analysis of data from carbon balances, that cell yields decreased during the later stages of anaerobic growth, possibly because of the accumulation of an inhibitory metabolite of one of the supplements (Keevil, Hough & Cole 1976).…”

Section: Introductionmentioning

confidence: 99%

Prototrophic Growth of Citrobacter freundii and the Biochemical Basis for its Apparent Growth Requirements in Aerated Media

Keevil

Hough

Cole

1977

Journal of General Microbiology

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“…Therefore, virulence factors homologous, and some even identical, to those described in E. coli pathotypes were detected in C. freundii strains isolated from sporadic cases of infantile diarrhea [26-29]. Additionally, isolates of C. freundii have been identified as effective recipient strains even since the first articles concerning E. coli conjugation mediated by F pili were published [30]. Reports on the transfer of E. coli thermo-stable toxin genes between these species raised considerations about the virulence potential of the bacterial conjugation [29,31,32].…”

Section: Introductionmentioning

confidence: 99%

Diarrhea-associated biofilm formed by enteroaggregative Escherichia coli and aggregative Citrobacter freundii: a consortium mediated by putative F pili

et al. 2010

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BackgroundEnteroaggregative Escherichia coli (EAEC) are enteropathogenic strains identified by the aggregative adhesion (AA) pattern that share the capability to form biofilms. Citrobacter freundii is classically considered as an indigenous intestinal species that is sporadically associated with diarrhea.ResultsDuring an epidemiologic study focusing on infantile diarrhea, aggregative C. freundii (EACF) and EAEC strains were concomitantly recovered from a severe case of mucous diarrhea. Thereby, the occurrence of synergic events involving these strains was investigated. Coinfection of HeLa cells with EACF and EAEC strains showed an 8-fold increase in the overall bacterial adhesion compared with single infections (P < 0.001). The synergic effect was mediated by physical interactions among the bacteria and primed in the absence of chemical signaling and without the participation of host cells. Thus, significant increases (2.7-fold on average) in bacterial adhesion were also observed during the formation of mixed biofilms on abiotic surfaces. Bacterial settling assays showed that EAEC strains harboring F-pili genes (traA) were capable of forming bacterial aggregates only in the presence of EACF. Scanning electronic microscopy analyses revealed that bacterial aggregates as well as enhanced biofilms formed by EACF and traA-positive EAEC were mediated by non-bundle forming, flexible pili. Moreover, mixed biofilms formed by EACF and traA-positive EAEC strains were significantly reduced using nonlethal concentration of zinc, a specific inhibitor of F pili. In addition, EAEC strains isolated from diarrheic children frequently produced single biofilms sensitive to zinc.ConclusionsPutative F pili expressed by EAEC strains boosted mixed biofilm formation when in the presence of aggregative C. freundii.

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Citrobacter freundii Mutants Deficient in Host Specificity Functions and Their Recipient Ability for Foreign Deoxyribonucleic Acid

Cited by 16 publications

References 32 publications

Restriction of DNA in Yersinia enterocolitica Detected Recipient Ability for a Derepressed R Factor from Escherichia coli

Restriction of DNA in Yersinia enterocolitica Detected Recipient Ability for a Derepressed R Factor from Escherichia coli

Prototrophic Growth of Citrobacter freundii and the Biochemical Basis for its Apparent Growth Requirements in Aerated Media

Diarrhea-associated biofilm formed by enteroaggregative Escherichia coli and aggregative Citrobacter freundii: a consortium mediated by putative F pili

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