Citrobacter freundii infection in silver catfish (Rhamdia quelen): Hematological and histological alterations

Bandeira, Guerino; Santos, Alessandro Casale dos; Souza, Carine de Freitas; Baldissera, Matheus D.; Moreira, Karen Luise dos Santos; Veiga, Marcelo Leite da; Rocha, Maria Izabel de Ugalde Marques da; Vargas, Águeda Castagna de; Cunha, Mauro Alves da; Baldisserotto, Bernardo

doi:10.1016/j.micpath.2018.09.038

Cited by 38 publications

(13 citation statements)

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“…Hematological parameters, as the main indicators of fish health status, are sensitive to bacterial infections [ 37 ]. Bacterial infections can adversely affect the oxygen-carrying capacity of the blood and the electrolyte balance of the blood, leading to extravasation of red blood cells and changes in cell size [ 38 ]. Therefore, hematological parameters are often used as important indicators to assess the health status of fish after exposure to bacterial infections and various other environmental stresses [ 38 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

“…Bacterial infections can adversely affect the oxygen-carrying capacity of the blood and the electrolyte balance of the blood, leading to extravasation of red blood cells and changes in cell size [ 38 ]. Therefore, hematological parameters are often used as important indicators to assess the health status of fish after exposure to bacterial infections and various other environmental stresses [ 38 , 39 ]. ROS, GLU, MDA, LZM, ALT, and AST are common serum examination parameters [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

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ROS Induced by Streptococcus agalactiae Activate Inflammatory Responses via the TNF-α/NF-κB Signaling Pathway in Golden Pompano Trachinotus ovatus (Linnaeus, 1758)

et al. 2022

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Streptococcus agalactiae is common pathogenic bacteria in aquaculture and can cause mass mortality after fish infection. This study aimed to investigate the effects of S. agalactiae infection on the immune and antioxidant regulatory mechanisms of golden pompano (Trachinotus ovatus). Serum and liver samples were obtained at 0, 6, 12, 24, 48, 96, and 120 h after golden pompano infection with S. agalactiae for enzyme activity and gene expression analyses. After infection with S. agalactiae, the content of reactive oxygen species (ROS) in serum was significantly increased (p < 0.05). Serum levels of glucose (GLU), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and malondialdehyde (MDA) increased and then decreased (p < 0.05), reaching a maximum at 6 h. Serum antioxidant enzyme (LZM) activity increased significantly (p < 0.05) and reached a maximum at 120 h. In addition, the mRNA expression levels of antioxidant genes (SOD, CAT, and GPx) in the liver increased and then decreased, reaching the maximum at 24 h, 48 h, and 24 h, respectively. During the experimental period, the mRNA expression levels of NF-κB-related genes of the inflammatory signaling pathway inhibitory κB (IκB) showed an overall decreasing trend (p < 0.05) and the lowest expression at 120 h, whereas the mRNA expression levels of tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), IκB kinase (IKK), and nuclear factor NF-κB increased significantly (p < 0.05) and the highest expression was at 120 h. In conclusion, these results showed that S. agalactiae could activate internal regulatory signaling in the liver of golden pompano to induce defense and immune responses. This study is expected to lay a foundation to develop the healthy aquaculture of golden pompano and promote a more comprehensive understanding of its disease resistance mechanisms.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

ROS Induced by Streptococcus agalactiae Activate Inflammatory Responses via the TNF-α/NF-κB Signaling Pathway in Golden Pompano Trachinotus ovatus (Linnaeus, 1758)

et al. 2022

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“…Several C. freundii strains contain flagella used for locomotion while some are non-motile. Citrobacter freundii is closely related to E.coli and Salmonella and is prevalent in diverse ecological niches such as soil, water, sewage, food and the gut of animals including humans ( Knirel et al, 2002 ; Murray et al, 2010 ; Abbott, 2011 ; Bandeira Junior et al, 2018 ). Citrobacter freundii , a causative agent of gastroenteritis and hemorrhagic septicemia, is a common pathogen of diverse freshwater fish where infected fish show symptoms such as skin ulceration, systemic infectious signs on the liver, kidney, muscles and gills ( Jeremić et al, 2003 ; Baldissera et al, 2018 ; Sun et al, 2018 ).…”

Section: Proteobacteria (Class: Gammaproteobacteria)mentioning

confidence: 99%

Secretion Systems in Gram-Negative Bacterial Fish Pathogens

Mekasha

Linke

2021

Front. Microbiol.

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Bacterial fish pathogens are one of the key challenges in the aquaculture industry, one of the fast-growing industries worldwide. These pathogens rely on arsenal of virulence factors such as toxins, adhesins, effectors and enzymes to promote colonization and infection. Translocation of virulence factors across the membrane to either the extracellular environment or directly into the host cells is performed by single or multiple dedicated secretion systems. These secretion systems are often key to the infection process. They can range from simple single-protein systems to complex injection needles made from dozens of subunits. Here, we review the different types of secretion systems in Gram-negative bacterial fish pathogens and describe their putative roles in pathogenicity. We find that the available information is fragmented and often descriptive, and hope that our overview will help researchers to more systematically learn from the similarities and differences between the virulence factors and secretion systems of the fish-pathogenic species described here.

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“…The inoculum was prepared for the infection model in sterile saline (NaCl 0.9%) with the turbidity adjusted to an of OD 600 0.8, equivalent to 6.4x10 8 CFU mL -1 . The uninfected groups were inoculated intramuscularly into the right dorsal region with 100 μL of sterile saline, while the infected groups were inoculated with 100 μL of the inoculum, according to methods previously described (BANDEIRA JUNIOR et al, 2018a). The inoculated fish were distributed into 30 L tanks and submitted to daily baths of 1 h, with the different testing solutions, for 4-days, consecutively.…”

Section: In Vivo Experimentsmentioning

confidence: 99%

Plant essential oils against bacteria isolated from fish: an in vitro screening and in vivo efficacy of Lippia origanoides

et al. 2019

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The use of natural products, such as essential oils (EOs), is a potential novel approach to treat fish bacterial infections with a lower risk of developing resistance. There has been a number of studies reporting the activity of EOs as those obtained from the species Achyrocline satureioides, Aniba parviflora, Aniba rosaeodora, Anthemis nobilis, Conobea scoparioides, Cupressus sempervirens, Illicium verum, Lippia origanoides, and Melaleuca alternifolia against bacteria. However, there are few studies investigating the effect of these EOs against fish bacteria. Therefore, the aim of this study was to evaluate the in vitro antibacterial activity of EOs against the following fish bacteria, Aeromonas hydrophila, Citrobacter freundii, and Raoultella ornithinolytica. Additionally, the in vivo antibacterial activity of the EO L. origanoides was evaluated against experimentally induced A. hydrophila infection of silver catfish (Rhamdia quelen). The EO of L. origanoides was chosen as it showed the highest in vitro antibacterial activity, with minimum inhibitory concentrations ranging from 0.2 to 0.8 mg mL-1. This EO also presented a therapeutic success of 58.33%, on a 30 day A. hydrophila infection. Therefore, we suggested that the EO of L. origanoides may be a viable alternative as a treatment for A. hydrophila infection.

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Citrobacter freundii infection in silver catfish (Rhamdia quelen): Hematological and histological alterations

Cited by 38 publications

References 20 publications

ROS Induced by Streptococcus agalactiae Activate Inflammatory Responses via the TNF-α/NF-κB Signaling Pathway in Golden Pompano Trachinotus ovatus (Linnaeus, 1758)

ROS Induced by Streptococcus agalactiae Activate Inflammatory Responses via the TNF-α/NF-κB Signaling Pathway in Golden Pompano Trachinotus ovatus (Linnaeus, 1758)

Secretion Systems in Gram-Negative Bacterial Fish Pathogens

Plant essential oils against bacteria isolated from fish: an in vitro screening and in vivo efficacy of Lippia origanoides

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