Citrobacter braakii carrying plasmid-borne mcr-1 colistin resistance gene from ready-to-eat food from a market in the Chaco region of Bolivia

Sennati, Samanta; Pilato, Vincenzo Di; Riccobono, Eleonora; Maggio, Tiziana Di; Villagrán, Ana Liz; Pallecchi, Lucia; Bartoloni, Alessandro; Rossolini, Gian María; Giani, Tommaso

doi:10.1093/jac/dkx078

Cited by 42 publications

(26 citation statements)

References 12 publications

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“…(Zhao and Zong, 2016), and Citrobacter spp. (Li et al, 2017;Sennati et al, 2017). The mcr-1 gene has been reported to exist in different food animals, such as in pigs (Meinersmann et al, 2017;Roschanski et al, 2017), poultry (Lima Barbieri et al, 2017;Monte et al, 2017) and cattle (Huang et al, 2017).…”

Section: Use Of Colistin In Animal Productionmentioning

confidence: 99%

Plasmid-mediated colistin resistance in animals: current status and future directions

Sun

Zeng

et al. 2017

Anim. Health. Res. Rev.

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Colistin, a peptide antibiotic belonging to the polymyxin family, is one of the last effective drugs for the treatment of multidrug resistant Gram-negative infections. Recent discovery of a novel mobile colistin resistance gene, mcr-1, from people and food animals has caused a significant public health concern and drawn worldwide attention. Extensive usage of colistin in food animals has been proposed as a major driving force for the emergence and transmission of mcr-1; thus, there is a worldwide trend to limit colistin usage in animal production. However, despite lack of colistin usage in food animals in the USA, mcr-1-positive Escherichia coli isolates were still isolated from swine. In this paper, we provided an overview of colistin usage and epidemiology of mcr-1 in food animals, and summarized the current status of mechanistic and evolutionary studies of the plasmid-mediated colistin resistance. Based on published information, we further discussed several non-colistin usage risk factors that may contribute to the persistence, transmission, and emergence of colistin resistance in an animal production system. Filling the knowledge gaps identified in this review is critical for risk assessment and risk management of colistin resistance, which will facilitate proactive and effective strategies to mitigate colistin resistance in future animal production systems.

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Section: Use Of Colistin In Animal Productionmentioning

confidence: 99%

Plasmid-mediated colistin resistance in animals: current status and future directions

Sun

Zeng

et al. 2017

Anim. Health. Res. Rev.

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“…Since its first discovery in Southern China in the late of 2015 [6], the mcr-1 gene have been detected in nearly 40 countries from five of seven continents [7, 20]. The mcr-1 -positive gut bacteria included Escherichia coli ( E. coli ) [21, 22], S. enterica [23, 24], K. pneumoniae [6, 25, 26], Enterobacter aerogenes ( E. aerogenes ) [27], Kluyvera ascorbata [28], Citrobacter freundii [29], and Citrobacter braakii [30]. Not only does the mcr-1 gene appear in diversified E. coli isolates with different sequence types [20, 31], but also it coexists with the NDM-1 [32, 33] and its variants, like NDM-5 [34, 35] and NDM-9 [36].…”

Section: Introductionmentioning

confidence: 99%

Expanding landscapes of the diversified mcr-1-bearing plasmid reservoirs

Wang

Sun

et al. 2017

Microbiome

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BackgroundPolymyxin is a cationic polypeptide antibiotic that can disrupt bacterial cell membrane by interacting with its lipopolysaccharide molecules and is used as a last resort drug against lethal infections by the carbapenem-resistant superbugs (like NDM-1). However, global discovery of the MCR-1 colistin resistance dramatically challenges the newly renewed interest in colistin for clinical use.MethodsThe mcr-1-harboring plasmids were acquired from swine and human Escherichia coli isolated in China, from 2015 to 2016, and subjected to Illumina PacBio RSII and Hi-Seq2000 for full genome sequencing. PCR was applied to close the gap of the assembled contigs. Ori-Finder was employed to predict the replication origin (oriC) in plasmids. The phenotype of MCR-1-producing isolates was evaluated on the LBA plates with various level of colistin. Genetic deletion was used to test the requirement of the initial “ATG” codon for the MCR-1 function.ResultsHere, we report full genomes of over 10 mcr-1-harboring plasmids with diversified replication incompatibilities. A novel hybrid IncI2/IncFIB plasmid pGD17-2 was discovered and characterized from a swine isolate with colistin resistance. Intriguingly, co-occurrence of two unique mcr-1-bearing plasmids (pGD65-3, IncI2, and pGD65-5, IncX4) was detected in a single isolate GD65, which might accelerate dissemination of the mcr-1 under environmental selection pressure. Genetic analyses of these plasmids mapped mobile elements in the context of antibiotic resistance and determined two insertion sequences (ISEcp1 and ISApl1) that are responsible for the mobilization of mcr-1. Gene deletion also proved that the first ATG codon is redundant in the mcr-1 gene.ConclusionsCollectively, our results extend landscapes of the diversified mcr-1-bearing plasmid reservoirs.Electronic supplementary materialThe online version of this article (doi:10.1186/s40168-017-0288-0) contains supplementary material, which is available to authorized users.

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“…Very recently, the presence of mcr-1 on a conjugative IncI2 plasmid from Citrobacter braakii was reported in Bolivia, indicating that Citrobacter spp. may be a new reservoir of mcr-1 [2]. Here we report a Citrobacter freundii strain possessing the mcr-1 gene on an IncHI2 plasmid.…”

Section: Emergence Of the Colistin Resistance Gene Mcr-1 In Citrobactmentioning

confidence: 95%

Emergence of the colistin resistance gene mcr-1 in Citrobacter freundii

Fang

Jiang

et al. 2017

International Journal of Antimicrobial Agents

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Citrobacter braakii carrying plasmid-borne mcr-1 colistin resistance gene from ready-to-eat food from a market in the Chaco region of Bolivia

Cited by 42 publications

References 12 publications

Plasmid-mediated colistin resistance in animals: current status and future directions

Plasmid-mediated colistin resistance in animals: current status and future directions

Expanding landscapes of the diversified mcr-1-bearing plasmid reservoirs

Emergence of the colistin resistance gene mcr-1 in Citrobacter freundii

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