Citric acid production from raw glycerol by acetate mutants of Yarrowia lipolytica

Rymowicz, Waldemar; Rywińska, Anita; Żarowska, Barbara; Juszczyk, Piotr

doi:10.2478/s11696-006-0071-3

Cited by 100 publications

(89 citation statements)

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“…A. niger are principally used to produce citric acid, however in culturing these fungi, the growth of Aspergillus in pellet form is desirable and this can be achieved by process optimization [5,6]. Yarrowia lipolytica has the ability to produce citric acid from raw glycerol [7], a by-product of biodiesel production from rapeseed oil. Citric acid is produced commercially employing various inexpensive and readily available raw materials like molasses, carob pod extract, rape seed oil, corn-cobs, apple and grape pomace, kiwi-fruit peel, mandarine orange and brewery wastes have been used as carbon substrates [8] in terrestrial environment.…”

Section: Introductionmentioning

confidence: 99%

An Experimental Study on Citric Acid Production by Aspergillus niger Using Gelidiella acerosa as a Substrate

Ramesh

Kalaiselvam

2011

Indian J Microbiol

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Citric acid (CA) is the most important commercial product which is produced by using various sugar substrates in the terrestrial environment. The present study made an attempt to produce citric acid by the fungal strain Aspergillus niger from red seaweed Gelidiella acerosa is the best alternative to sugar substrate in the marine environment. In this study three types of production media were prepared including control (sucrose) by following standard fermentation conditions. The acid production was indicated by the reduction of pH levels. The control medium gave the highest yield of 80 g/l at pH 1.5 and the medium containing crude seaweed powder and other compositions gave the yield of 30 g/l at pH 3.5 whereas the medium containing crude seaweed and 10% sucrose gave the yield of 50 g/l at pH 3.0. When calculating the benefit cost ratio, crude seaweed powder and 10% sucrose yielded 50 g of citric acid at the lower cost of Rs. 35, whereas the other two media gave the yield of 80 and 30 g respectively with the cost of Rs. 77 and 28. In economic point of view, the medium containing seaweed and 10% sucrose showed more benefit with lower cost.

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Section: Introductionmentioning

confidence: 99%

An Experimental Study on Citric Acid Production by Aspergillus niger Using Gelidiella acerosa as a Substrate

Ramesh

Kalaiselvam

2011

Indian J Microbiol

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“…Glycerol may be used as carbon source in many bioprocesses, and one promising exploitation is to produce 1,3-PDO by Klebsiella pneumoniae and Clostridium butyricum (Papanikolaou and Aggelis, 2009;Rymowicz et al, 2006). Production from crude glycerol from biodiesel can contribute to the reduction of environmental pollution and commercial valorization of this carbon source and to lower the production of 1,3-propanediol.…”

Section: Introductionmentioning

confidence: 99%

Anaerobic and micro-aerobic 1,3-propanediol production by engineered Escherichia coli with dha genes from Klebsiella pneumoniae GLC29

Neto¹,

Briganti²,

Layton³

et al. 2017

Afr. J. Biotechnol.

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1,3-Propanediol (1,3-PDO) is a bifunctional molecule, and used in applications similar to those of ethylene glycol, propylene glycol, 1,3-butanediol and 1,4-butanediol. The use of glycerol as a feedstock is an alternative to reduce production costs for both 1,3-PDO and biodiesel, since biodiesel glycerol can be used for the production of 1,3-PDO by bacteria. Also, using metabolic engineering, it is possible to manipulate the metabolic routes and obtain high value products, reduce or eliminate the formation of undesirable byproducts. The aim of the study was to produce 1,3-propanediol in E. coli cloned with dha genes from Klebsiella pneumoniae GLC29. Six genes responsible for 1,3-PDO production in Klebsiella pneumoniae GLC29 were cloned. These genes were assembled in pSB1C3 as an expression vector: Genes dhaB1, dhaB2, dhaB3 and dhaT (pSB1C3dhaB123T), and another vector with genes dhaF and dhaG (pSB1C3dhaB123TFG) were derived using Gibson's Assembly technique. Escherichia coli TCS099 and SZ63 stains were used as hosts for 1,3-PDO production, and kept at -80°C for long-term storage. Glycerol was used as the sole or main carbon source in all experiments. Fermentations were performed in flasks in aerobic and anaerobic conditions using minimal media. Also, two stage fermentation (aerobic-anaerobic) was performed for 1,3-propanediol production. Only pSB1C3dhaB123TFG was able to produce high amounts of 1,3-PDO in shake flasks experiments, producing 2.5 g/L in micro-aerobic conditions, using E. coli TCS099 as host. Besides, E. coli SZ63 hosting pSB1C3dhaB123TFG was able to produce high amounts of 1,3-PDO, corresponding to 11.3 g/L of 1,3-PDO using a two-stage fermentation process using low concentration of vitamin B12 (1 mg/L). Plasmid pSB1C3dhaB123TFG shows potential for producing high amounts of 1,3-PDO, specially because of dhaF and dhaG, reaffirming the importance of this genes on 1,3-PDO production, especially with the addition of low amounts of vitamin B12, which is an expensive compound.

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“…54 321 Accordingly, higher glycerol above 60 g/L is responsible to induce higher osmotic pressure 322 which could inhibit the oxygen uptake or create high osmotic pressure sufficient to inhibit 323 culture growth in other strains. 18 More often, Rymowicz et al (2006) have 328 17 found that Y. lipolytica mutants can be cultivated in the presence of raw glycerol at extremely 329 high concentrations (i.e. 200 g/L) and can achieve efficient cell growth ranging from 16.5-330 26.5 g/L.…”

Section: Optimization Study 154mentioning

confidence: 99%

Lipid production by Yarrowia lipolytica grown on biodiesel-derived crude glycerol: optimization of growth parameters and their effects on the fermentation efficiency

2016

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21Yarrowia lipolytica, a well-known oleaginous strain for single cells oil (SCO) production was 22 grown in nitrogen-limited flask cultures. The effect of increasing the initial crude glycerol and 23 nitrogen concentration was studied along fermentation process. Significant biomass and SCO 24 production was reported with high initial glycerol concentration of 89 g/L and 0.54 g NH 4 OH/L 25 during 66 h. Optimized culture conditions were tested using 5-L fermenter during two-stage 26 cultivation with a dissolved oxygen shift from 60% to 30% of dissolved oxygen corresponding to 27 50-80 h -1 . Lipid concentration of 13.6 ± 0.8 g/L and lipid content 52.7 ± 1.2% (w/w of dry 28 biomass) was obtained which is higher compared with literature values for Yarrowia species 29 grown on crude glycerol based media. The yeast lipids contained mainly oleic, palmitic, linoleic 30 and stearic acids which could serve as perfect precursors for the synthesis of biodiesel. 31 32

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Citric acid production from raw glycerol by acetate mutants of Yarrowia lipolytica

Cited by 100 publications

References 1 publication

An Experimental Study on Citric Acid Production by Aspergillus niger Using Gelidiella acerosa as a Substrate

An Experimental Study on Citric Acid Production by Aspergillus niger Using Gelidiella acerosa as a Substrate

Anaerobic and micro-aerobic 1,3-propanediol production by engineered Escherichia coli with dha genes from Klebsiella pneumoniae GLC29

Lipid production by Yarrowia lipolytica grown on biodiesel-derived crude glycerol: optimization of growth parameters and their effects on the fermentation efficiency

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