Citrate uptake in membrane vesicles of Klebsiella aerogenes

Johnson, C L; dma, Y A; Stern, Joseph R.

doi:10.1128/jb.121.2.682-687.1975

Cited by 23 publications

(6 citation statements)

References 18 publications

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“…Na+-Coupled Transport Na + -solute symport. Different citrate transport systems are expressed in K. pneurnoninae when the bacteria are grown on citrate under aerobic or anaerobic conditions (49,96,215). The most prominent difference between these systems is a specific requirement for Na+ or Li' by the anaerobic citrate transport system.…”

Section: Na+ Gradient As Driving Force For Endergonic Membrane Reactionsmentioning

confidence: 99%

Sodium ion transport decarboxylases and other aspects of sodium ion cycling in bacteria.

Dimroth¹

1987

Microbiological Reviews

163

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Section: Na+ Gradient As Driving Force For Endergonic Membrane Reactionsmentioning

confidence: 99%

Sodium ion transport decarboxylases and other aspects of sodium ion cycling in bacteria.

Dimroth¹

1987

Microbiological Reviews

163

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“…Most of the enterobacteria which are able to utilize citrate possess an inducible citrate transport system under aerobic as well as under anaerobic conditions [5,6,[85][86][87][88]98,99]. Bacteria like E. aerogenes, Aerobacter indologenes, A. cloacae, Proteus rettgeri and Klebsiella pneumoniae, therefore, are able to utilize citrate aerobically as well as anaerobically.…”

Section: Citrate Metabolism In Enteric Bacteriamentioning

confidence: 99%

Citrate metabolism in anaerobic bacteria

Antranikian

Giffhorn

1987

FEMS Microbiology Letters

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The regulation of anaerobic citrate metabolism is very diverse among different groups of bacteria. In organisms like Streptococcus lactis and Clostridium sporosphaeroides which lack citrate synthase, the activity of its antagonistic enzyme, citrate lyase, need not be regulated. Many anaerobes like Rhodocyclus gelatinosus and Clostridium sphenoides are able to synthesize their own l‐glutamate and contain citrate synthase. In these bacteria the activity of citrate metabolizing enzymes which are involved in a cascade system are under strict control. In Rc. gelatinosus activation/inactivation of citrate lyase is controlled by acetylation/deacetylation which is catalyzed by its corresponding regulatory enzymes, citrate lyase ligase and citrate lyase deacetylase. In C. sphenoides inactivation of citrate lyase is accomplished by deacetylation as well as by changing in the enzyme conformation. Activation of citrate lyase is catalyzed by citrate lyase ligase whose activity in addition is modulated by phosphorylation/dephosphorylation. Further, electron transport process also seems to play a role in the inactivation of citrate metabolizing enzymes in enteric bacteria.

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“…Another curious phenomenon which became evident from these studies was that intact cells responded differently to the artificial electron donor'system, PMS-ascorbate, than membrane vesicles isolated from the same bacterial species (3,5, 17,18), and to TMPD-ascorbate, which has been shown to be a satisfactory substitute for PMS (19,20). Thus, on the one hand, PMS-ascorbate energizes active transport in isolated membrane vesicles while, on the other hand, PMS-ascorbate inhibits active transport by intact cells from which the membrane vesicles were prepared.…”

Section: Effect Of Pms-ascorbate On the Phosphoenopyruvate (Pep) Phosmentioning

confidence: 99%

The inhibitory effect of the artificial electron donor system, phenazine methosulfate‐ascorbate, on bacterial transport mechanisms

1977

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The artificial electron donor system, phenazine methosulfate (PMS)-ascorbate, inhibited active transort of solutes in Pseudomonas aeruginosa irrespective of whether the active transport systems were shock sensitive or shock resistant. N,N,N',N'-tetramethylphenylenediamine could be substituted for PMS but a higher concentration was required. PMS-ascorbate also inhibited active transport in several other bacterial species with the exception of Escherichia coli and of a nonpigmented strain of Serratia marcescens. PMS-ascorbate previously has been shown to energize active transport in isolated membrane vesicles, even those prepared from the same bacterial species in whose intact cells active transport was inhibited. The apparent Km of glucose active transport in untreated cells of P. aeruginosa was 40 micron while the Km of glucose transport in cells incubated with PMS-ascorbate was 25 mM, and PMS-ascorbate had no effect on efflux of accumulated glucose. These results strongly suggested that facilitated diffusion resulted upon exposure of the cells to PMS- ascorbate. Thus, PMS-ascorbate appeared to have an uncoupler-like effect on cells of P. aeruginosa. The experimental data also pointed out that there are fundamental differences between the response of intact cells and membrane vesicles to exogenous electron donors.

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Citrate uptake in membrane vesicles of Klebsiella aerogenes

Cited by 23 publications

References 18 publications

Sodium ion transport decarboxylases and other aspects of sodium ion cycling in bacteria.

Sodium ion transport decarboxylases and other aspects of sodium ion cycling in bacteria.

Citrate metabolism in anaerobic bacteria

The inhibitory effect of the artificial electron donor system, phenazine methosulfate‐ascorbate, on bacterial transport mechanisms

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