Citral mitigates inflammation of Caco-2 cells induced by <i>Cronobacter sakazakii</i>

Guo, Du; Bai, Fangting; Zhan, Xiangjun; Zhang, Wenting; Tong, Jin; Wang, Yutang; Xia, Xiaodong; Shi, Chao

doi:10.1039/d2fo00098a

Cited by 4 publications

(3 citation statements)

References 44 publications

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“…Citral inhibited overexpression of several pro‐inflammatory genes including TNF‐α and IL‐6 tested in our study too (Campos et al., 2019; Charret et al., 2021; Emilio‐Silva et al., 2020; Shi et al., 2020). In addition to such suppression of inflammatory factors, a decrease in expression of proteins associated with inflammatory signaling pathways has been observed in vitro suggesting the potential of citral to prevent inflammation (Guo et al., 2022).…”

Section: Discussionmentioning

confidence: 99%

Anti‐inflammatory properties of an aldehydes‐enriched fraction of grapefruit essential oil

Nikolić

Bosco

Moschetti

et al. 2023

Journal of Food Science

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Chronic inflammation is linked to the development of numerous diseases and is accompanied by increased cytokine secretion. Macrophages provide a first line of defense against pathogens that under inflammatory stimuli release pro‐inflammatory cytokines. The essential oil (EO) fractions obtained from Citrus spp. rich in different compounds have gained the attention of both researchers and users during the last decades. In particular, grapefruit (Citrus paradisi) peel is rich in phenolics and flavonoids with several health benefits, including anti‐inflammatory actions. Additionally, its EO consists of a large number of compounds such as monoterpenes, sesquiterpenes, alcohols, aldehydes, esters, and oxides. Among the methods for encapsulating EOs, spray‐drying is the main one. In the present study, we aimed to determine the in vitro anti‐inflammatory activity of EO from C. paradisi (grapefruit essential oil [GEO]) (whole and fractions) in a lipopolysaccharide (LPS)‐induced inflammation model. Results indicate that Fr‐GEO and Fr‐GEO_SD exert protective effects against LPS‐induced inflammation by decreasing gene expression and levels of pro‐inflammatory cytokines as IL‐6 and TNF‐α. Monoterpenes as the most common components, as well as aldehydes and sesquiterpenes, might be responsible for such effects, although a synergistic action is not excluded. Furthermore, a higher percent of aldehydes is linked to improved olfactory properties. Our findings support the anti‐inflammatory effects of selected Fr‐GEO with a great potential for the development of new nutraceuticals and/or functional food for the treatment of inflammatory‐associated diseases. Practical Application The findings of this study support the anti‐inflammatory effects of selected Fr‐GEO with a great potential for the development of new nutraceuticals and/or functional food for the treatment of inflammatory‐associated diseases.

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Section: Discussionmentioning

confidence: 99%

Anti‐inflammatory properties of an aldehydes‐enriched fraction of grapefruit essential oil

Nikolić

Bosco

Moschetti

et al. 2023

Journal of Food Science

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“…The adhesion inhibitory effect was determined according to the method of Guo et al’s ( 2022 ) with slight alterations. The adhesion inhibitory effect of L. monocytogenes on Caco-2 cells was assessed by competition, exclusion, and displacement experiments using three bacteriocins.…”

Section: Methodsmentioning

confidence: 99%

Bacteriocins attenuate Listeria monocytogenes–induced intestinal barrier dysfunction and inflammatory response

Wang,

Du,

et al. 2024

Appl Microbiol Biotechnol

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Bacteriocins have the potential to effectively improve food-borne infections or gastrointestinal diseases and hold promise as viable alternatives to antibiotics. This study aimed to explore the antibacterial activity of three bacteriocins (nisin, enterocin Gr17, and plantaricin RX-8) and their ability to attenuate intestinal barrier dysfunction and inflammatory responses induced by Listeria monocytogenes, respectively. Bacteriocins have shown excellent antibacterial activity against L. monocytogenes without causing any cytotoxicity. Bacteriocins inhibited the adhesion and invasion of L. monocytogenes on Caco-2 cells, lactate dehydrogenase (LDH), trans-epithelial electrical resistance (TEER), and cell migration showed that bacteriocin improved the permeability of Caco-2 cells. These results were attributed to the promotion of tight junction proteins (TJP) assembly, specifically zonula occludens-1 (ZO-1), occludin, and claudin-1. Furthermore, bacteriocins could alleviate inflammation by inhibiting the mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-κB) pathways and reducing the secretion of interleukin-6 (IL-6), interleukin-1 β (IL-1β) and tumor necrosis factor α (TNF-α). Among three bacteriocins, plantaricin RX-8 showed the best antibacterial activity against L. monocytogenes and the most pronounced protective effect on the intestinal barrier due to its unique structure. Based on our findings, we hypothesized that bacteriocins may inhibit the adhesion and invasion of L. monocytogenes by competing adhesion sites. Moreover, they may further enhance intestinal barrier function by inhibiting the expression of L. monocytogenes virulence factors, increasing the expression of TJP and decreasing the secretion of inflammatory factors. Therefore, bacteriocins will hopefully be an effective alternative to antibiotics, and this study provides valuable insights into food safety concerns. Key points • Bacteriocins show excellent antibacterial activity against L. monocytogenes • Bacteriocins improve intestinal barrier damage and inflammatory response • Plantaricin RX-8 has the best protective effect on Caco-2 cells damage Graphical Abstract

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“…Aside from the studies presented in Table 5, many others were found in the bibliographic search reporting the anti-inflammatory activity of monoterpenes by evaluating the expression of pro-inflammatory and/or anti-inflammatory mediators, as these are the most common endpoints found in the inflammatory response. Examples include carvacrol [165][166][167][168], 1,8-cineole [169,170], citral [171,172], isopulegol [173], linalool [174,175], linalyl acetate [174] and thymol [176][177][178].…”

Section: Geraniolmentioning

confidence: 99%

Plant Monoterpenes and Essential Oils as Potential Anti-Ageing Agents: Insights from Preclinical Data

Zuzarte,

Sousa,

Alves-Silva

et al. 2024

Biomedicines

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Ageing is a natural process characterized by a time-dependent decline of physiological integrity that compromises functionality and inevitably leads to death. This decline is also quite relevant in major human pathologies, being a primary risk factor in neurodegenerative diseases, metabolic disorders, cardiovascular diseases and musculoskeletal disorders. Bearing this in mind, it is not surprising that research aiming at improving human health during this process has burst in the last decades. Importantly, major hallmarks of the ageing process and phenotype have been identified, this knowledge being quite relevant for future studies towards the identification of putative pharmaceutical targets, enabling the development of preventive/therapeutic strategies to improve health and longevity. In this context, aromatic plants have emerged as a source of potential bioactive volatile molecules, mainly monoterpenes, with many studies referring to their anti-ageing potential. Nevertheless, an integrated review on the current knowledge is lacking, with several research approaches studying isolated ageing hallmarks or referring to an overall anti-ageing effect, without depicting possible mechanisms of action. Herein, we aim to provide an updated systematization of the bioactive potential of volatile monoterpenes on recently proposed ageing hallmarks, and highlight the main mechanisms of action already identified, as well as possible chemical entity–activity relations. By gathering and categorizing the available scattered information, we also aim to identify important research gaps that could help pave the way for future research in the field.

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Citral mitigates inflammation of Caco-2 cells induced by Cronobacter sakazakii

Abstract: The aim of this study was to explore the anti-inflammatory effect and mechanism of citral in Cronobacter sakazakii-stimulated Caco-2 cells. Safe doses of citral were first determined in Caco-2 cells....

Cited by 4 publications

References 44 publications

Anti‐inflammatory properties of an aldehydes‐enriched fraction of grapefruit essential oil

Anti‐inflammatory properties of an aldehydes‐enriched fraction of grapefruit essential oil

Bacteriocins attenuate Listeria monocytogenes–induced intestinal barrier dysfunction and inflammatory response

Plant Monoterpenes and Essential Oils as Potential Anti-Ageing Agents: Insights from Preclinical Data

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