CITED4 gene silencing in colorectal cancer cells modulates adherens/tight junction gene expression and reduces cell proliferation

Rogers, Michael A.; Kalter, Verena; Marcias, Gemma; Zapatka, Marc; Barbus, Sebastian; Lichter, Peter

doi:10.1007/s00432-015-2011-5

Cited by 15 publications

(25 citation statements)

References 53 publications

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“…CITED4 interacts with CBP/p300, as well as the transcription factor AP‐2 (TFAP2), mediating TFAP2‐dependent transcription . Notably, CITED4 is downregulated in several cancer types, including colorectal cancer, brain cancer, and breast cancer, and contributes to the acquisition of malignant characteristics such as proliferation, survival, angiogenesis, and adherens junctions/tight junctions . Furthermore, Fox et al showed that the expression of CITED4 can be downregulated or translocated to the cytoplasm in breast cancer and inhibits HIF‐1α‐mediated transcription and angiogenesis through direct competition of p300 binding.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Copy Number Variations in Oral Cavity Squamous Cell Carcinoma Reveals a Novel Role for MLLT3 in Cell Invasiveness

et al. 2019

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Background. DNA copy number variations (CNVs) are a hallmark of cancer, and the current study aimed to demonstrate the profile of the CNVs for oral cavity squamous cell carcinoma (OSCC) and elucidate the clinicopathological associations and molecular mechanisms of a potential marker derived from CNVs, mixed-lineage leukemia translocated to chromosome 3 protein (MLLT3), in OSCC carcinogenesis. Materials and Methods. CNVs in 37 OSCC tissue specimens were analyzed using a high-resolution microarray, the OncoScan array. Gene expression was analyzed by real-time polymerase chain reaction in 127 OSCC and normal tissue samples. Cell function assays included cell cycle, migration, invasion and chromatin immunoprecipitation assays. Results. We found a novel copy number amplified region, chromosome 9p, encompassing MLLT3 via the comparison of our data set with six other OSCC genome-wide CNV data sets. MLLT3 overexpression was associated with

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Section: Discussionmentioning

confidence: 99%

Characterization of Copy Number Variations in Oral Cavity Squamous Cell Carcinoma Reveals a Novel Role for MLLT3 in Cell Invasiveness

et al. 2019

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“…The genes of interest were normalized against the housekeeping genes DCTN2 and PGK1. For a detailed protocol, see Rogers et al [18].…”

Section: Microarray Candidate Gene Validation By Qrt-pcrmentioning

confidence: 99%

“…The protein of interest was identified using Enhanced Chemiluminescence (ECL, Pierce) coupled with X-ray film exposure. For a detailed protocol, see Rogers et al [18].…”

Section: Western Blot Analysismentioning

confidence: 99%

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IGF2 knockdown in two colorectal cancer cell lines decreases survival, adhesion and modulates survival-associated genes

et al. 2016

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Increased expression of insulin-like growth factor 2 (IGF2) is found in tumors of colorectal cancer (CRC) patients exhibiting a gained region on chromosome 11q15 and is implicated in poor patient survival. This study analyzes in vitro phenotypic- and gene expression changes associated with IGF2 shRNA-mediated knockdown. Initially, doxycycline inducible IGF2 knockdown cell lines were generated in the CRC cell lines SW480 and LS174T. The cells were analyzed for changes in proliferation, cell cycle, apoptosis, adhesion, and invasion. Expression profiling analysis was performed, and, for a subset of the identified genes, expression was validated by qRT-PCR and Western blot. IGF2 knockdown inhibited cell proliferation in both cell lines induced G1 cell cycle blockade and decreased adhesion to several extracellular matrix proteins. Knockdown of IGF2 did not alter invasiveness in SW480 cells, while a slight increase in apoptosis was seen only in the LS174T cell line. Knockdown of IGF2 in SW480 deregulated 58 genes, several of which were associated with proliferation and cell-cell/cell-ECM contacts. A subset of these genes, including CDK2, YAP1, and BIRC5 (Survivin), are members of a common network. This study supports the concept of direct autocrine/paracrine tumor cell activation through IGF2 and a shows role of IGF2 in CRC proliferation, adhesion and, to a limited extent, apoptosis.

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“…Several reports have now pointed out the expression and the role of CITED proteins in stem cells (including ESC), in which they are involved in selfrenewal and cell fate decisions [38,[41][42][43][44][45][46]. Not surprisingly, due to the plethora of cellular and embryonic functions exerted by CITED1, CITED2 and CITED4, these genes were also showed to play intricate roles in tumorigenic processes [5,45,[47][48][49][50][51][52][53][54][55][56][57][58][59]. Therefore, CITED proteins are truly Regenerative Medicine Frontiers…”

Section: Introductionmentioning

confidence: 99%

CITED Proteins in the Heart of Pluripotent Cells and in Heart’s Full Potential

2019

Regen Med Front.

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The CITED family of transcriptional modulators plays multiple roles in the development of vertebrates. These proteins are characterized by the conservation of a carboxy-terminal domain which defines this family and has a high affinity for the transcriptional co-activators CBP/p300. In humans, mutations in some CITED genes or deregulation of their expression are associated with developmental anomalies. In particular, CITED2 dysfunction has been associated with congenital heart disease.Although most studies have reported the critical function of CITED proteins during development, there is increasing evidence supporting an important role for these proteins in physiological functions of adult organisms and pathologies such as cardiomyopathies and cancer. In addition, recent studies have pointed out the involvement of CITED proteins in embryonic and adult stem cells self-renewal, and cell fate decisions. Here, we present an overview of the CITED transcriptional modulators, their protein interactors and gene networks, with an emphasis on their importance in pluripotent stem cells and cardiogenesis.

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CITED4 gene silencing in colorectal cancer cells modulates adherens/tight junction gene expression and reduces cell proliferation

Cited by 15 publications

References 53 publications

Characterization of Copy Number Variations in Oral Cavity Squamous Cell Carcinoma Reveals a Novel Role for MLLT3 in Cell Invasiveness

Characterization of Copy Number Variations in Oral Cavity Squamous Cell Carcinoma Reveals a Novel Role for MLLT3 in Cell Invasiveness

IGF2 knockdown in two colorectal cancer cell lines decreases survival, adhesion and modulates survival-associated genes

CITED Proteins in the Heart of Pluripotent Cells and in Heart’s Full Potential

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