Cistanche deserticola polysaccharides protects PC12 cells against OGD/RP-induced injury

Liu, Yue; Wang, Hui; Yang, Mei; Liu, Ning; Zhao, Yuping; Qi, Xue; Niu, Yang; Sun, Tao; Li, Yuxiang; Yu, Jian‐Qiang

doi:10.1016/j.biopha.2018.01.114

Cited by 26 publications

(10 citation statements)

References 44 publications

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“…Oxygen glucose deprivation/reperfusion (OGD/P) experiments were performed as literature. 22 Cortical neurons were exposed to glucose-free aCSF solution. Then, it was incubated for 2 hours in an incubator of 5% CO 2 and 95% N 2 (OGD).…”

Section: Simulation Of I/r In Vitro and Gene Transfermentioning

confidence: 99%

<p>MicroRNA-579-3p Exerts Neuroprotective Effects Against Ischemic Stroke via Anti-Inflammation and Anti-Apoptosis</p>

Jia¹,

Cui²,

Tan³

et al. 2020

NDT

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Background/Aims: Multiple studies have found that microRNAs (miRNAs) are involved in the development of cerebral ischemia. MiR-579-3p can inhibit inflammatory responses and apoptosis, leading to ischemia/reperfusion (I/R) damage. However, the mechanism of how miR-579-3p actions in brain I/R injury remains unclear. This study aimed to investigate the mechanism of the role of miR-579-3p in brain I/R injury. Methods: A rat model of cerebral ischemia-reperfusion injury was established by suture method. The effects of miR-579-3p on cerebral infarction size, brain water content, and neurological symptoms were evaluated. Flow cytometry was used to detect apoptosis. ELISA was used to detect the level of inflammatory factors. Western blot was used to detect the expression of P65, NCOA1, Bcl-2 and Bax. The relationship between miR-579-3p and NCOA1 was analyzed by bioinformatics analysis and luciferase assay. Results: Overexpression of miR-579-3p reduced infarct volume, brain water content and neurological deficits. Overexpression of miR-579-3p inhibited the expression level of the inflammatory cytokines, such as TNF-α, IL-6, COX-2 and iNOS, and increased the expression level of IL-10. MiR-579-3p overexpression inhibited NF-кB activity by reducing NRIP1. In addition, miR-579-3p could reduce the apoptotic rate of cortical neurons. Overexpression of miR-579-3p inhibited the activity of caspase-3, increased the expression level of anti-apoptotic gene Bcl-2 in neurons, and decreased the expression level of apoptotic gene Bax. Conclusion: miR-579-3p can be used to treat brain I/R injury, and its neuroprotective effect may be ascribed to the reduction of inflammation and apoptosis.

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Section: Simulation Of I/r In Vitro and Gene Transfermentioning

confidence: 99%

<p>MicroRNA-579-3p Exerts Neuroprotective Effects Against Ischemic Stroke via Anti-Inflammation and Anti-Apoptosis</p>

Jia¹,

Cui²,

Tan³

et al. 2020

NDT

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“…Its components are useful in ethanol‐induced liver injury and intestinal inflammatory hyperplasia; it can also be used as an anti‐fatigue, anti‐inflammatory and anti‐tumour reagent . Recently, Guo et al reported that Cistanche deserticola polysaccharide (CDP), one of its main components, possessed antioxidant and hepatoprotective activity; another two studies identified its role in protecting cells from oxidative stress injury in oxygen‐glucose deprivation/reperfusion and osteoporosis conditions . However, the role of CDP in depigmentation diseases has not been elucidated.…”

Section: Introductionmentioning

confidence: 99%

Cistanche deserticola polysaccharide induces melanogenesis in melanocytes and reduces oxidative stress via activating NRF2/HO‐1 pathway

Huang

et al. 2020

J Cellular Molecular Medi

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As a main part of pigmentation disorders, skin depigmentation diseases such as vitiligo and achromic naevus are very common and get more attention now. The pathogenesis of depigmentation includes melanocyte dysfunction and loss, which are possibly caused by heredity, autoimmunity and oxidative stress. Among them, oxidative stress plays a key role; however, few clinical treatments can deal with oxidative stress. As reported, Cistanche deserticola polysaccharide (CDP) is an effective antioxidant; based on that, we evaluated its role in melanocyte and further revealed the mechanisms.In this study, we found that CDP could promote melanogenesis in human epidermal melanocytes (HEMs) and mouse melanoma B16F10 cells, it also induced pigmentation in zebrafish. Furthermore, CDP could activate mitogen-activated protein kinase (MAPK) signal pathway, then up-regulated the expression of microphthalmiaassociated transcription factor (MITF) and downstream genes TYR, TRP1, TRP2 and RAB27A. Otherwise, we found that CDP could attenuate H 2 O 2 -induced cytotoxicity and apoptosis in melanocytes. Further evidence revealed that CDP could enhance NRF2/HO-1 antioxidant pathway and scavenge intracellular ROS. In summary, CDP can promote melanogenesis and prevent melanocytes from oxidative stress injury, suggesting that CDP helps maintain the normal status of melanocytes. Thus, CDP may be a novel drug for the treatment of depigmentation diseases. K E Y W O R D S Cistanche deserticola polysaccharide, depigmentation disease, melanocyte, melanogenesis, NRF2, oxidative stress S U PP O RTI N G I N FO R M ATI O N Additional supporting information may be found online in the Supporting Information section. How to cite this article: Hu Y, Huang J, Li Y, et al. Cistanche deserticola polysaccharide induces melanogenesis in melanocytes and reduces oxidative stress via activating NRF2/HO-1 pathway.

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“…Total glycosides from C. deserticola exert protective effect against middle cerebral artery occlusion (MCAO)‐induced cerebral injury by downregulating Keap1 expression and facilitating Nrf2 nuclear translocation 118 . C. deserticola polysaccharides also show neuroprotective activity against oxygen‐glucose deprivation/reperfusion‐induced injury via inhibiting oxidative stress 119 . Moreover, both ECH and VB show protective potential against ischemia/reperfusion‐induced neuronal injury.…”

Section: Pharmacological Activitiesmentioning

confidence: 99%

Cistanches Herba, from an endangered species to a big brand of Chinese medicine

Song

Zeng

Jiang

et al. 2021

Medicinal Research Reviews

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Cistanches Herba (CH, Chinese name: Roucongrong), is a very precious, tonic Chinese medicine. Cistanche deserticola and Cistanche tubulosa are the two commonly used species and authenticated in Chinese Pharmacopoeia. Due to the parasitic nature of Cistanche plants, the wild source was once endangered and listed in the Appendix II of Convention on International Trade in Endangered Species of Wild Fauna and Flora. However, after continuously struggling in the past decades, CH has grown up to a big brand of Chinese medicine featured with the cultivation area as 1.26 million mu, the annual output as 6000 tons, and the related industrial output value as more than 20 billion China Yuan, attributing to large‐scale cultivation and in‐depth phytochemical and pharmacological investigations. Noteworthily, great achievements have reached concerning the research and development of relevant products, such as modern drugs, traditional Chinese medicine prescriptions, and dietary supplements. The current review summarizes the research progresses concerning the distribution and cultivation, phytochemistry, pharmacology, metabolism and product development of CH in the past decades, and the emerging challenges and developing prospects are discussed as well.

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Cistanche deserticola polysaccharides protects PC12 cells against OGD/RP-induced injury

Cited by 26 publications

References 44 publications

<p>MicroRNA-579-3p Exerts Neuroprotective Effects Against Ischemic Stroke via Anti-Inflammation and Anti-Apoptosis</p>

<p>MicroRNA-579-3p Exerts Neuroprotective Effects Against Ischemic Stroke via Anti-Inflammation and Anti-Apoptosis</p>

Cistanche deserticola polysaccharide induces melanogenesis in melanocytes and reduces oxidative stress via activating NRF2/HO‐1 pathway

Cistanches Herba, from an endangered species to a big brand of Chinese medicine

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