Cisplatin and Probiotic Biomass Loaded Pessaries for the Management of Cervical Cancer

Abstract

Introduction: Cancer is a type of disease, in which the growth of cells is abnormal and uncontrolled. One of the most common cancers among women is cervical cancer. In India, cervical cancer is one of leading causes of cancer mortality among women 30 to 69 years of age, accounting for 17% of all cancer deaths. The work present here shows the combined effects of anticancer drug along with probiotics to circumvent the side effects associated with chemotherapy and to enhance the therapeutic effect. Material and Methods: Cisplatin and drug loaded pessaries were prepared by melt mold method using the blend of PEG’s (Polyethylene Glycol) and further characterized for various in vitro and in vivo parameters. Result and Discussion: The free radical scavenging activity of probiotic Lactobacillus rhamnosus by DPPH (2,2-diphenyl-1-picrylhydrazyl) assay was observed to be 60.77μg/mL The mean weight variation, melting time, content uniformity, friability and hardness of the prepared pessary were 1.25±0.025mg, 10.86±0.64min, 99.89±0.74, 0.25%, 2.2kg/cm2. Histopathology studies presented that the developed formulation are safe for local delivery of cisplatin. Conclusion: This study provides the basis for a combination of local delivery approach along with the beneficial effects of probiotic strain which could be better a approach for the treatment of cervical cancer.

The role of probiotics in vaginal health

“…In another meta-analysis ( Qiu et al., 2019 ) comparing the incidence of probiotics in the prevention of diarrhea caused by cervical cancer radiation therapy, the probiotics group had a lower incidence of RID, RR 0.61 (95% CI 0.46-0.81; P = 0.0007). Negi et al ( Negi et al., 2020 ) developed cisplatin and probiotic bioburden pessaries for the treatment of cervical cancer. Histopathological studies showed that the preparation was safe for local administration of cisplatin.…”

Relationship between Human Papillomavirus Status and the Cervicovaginal Microbiome in Cervical Cancer

“…For instance, cisplatin is a common therapy for CC; however, its toxicity affects normal cells and tissues. Negi et al [ 116 ] combined cisplatin with probiotic-loaded pessaries (Lactobacillus rhamnosus) in a vaginal mouse model. They found a better outcome with fewer side effects of cisplatin and a reduced tumor volume in the treated group.…”

Vaginal microbes confounders and implications on women's health

“…Probiotics were also used for their anticancer activities in cervical cancer via activating the maturation of natural killer cells and promoting cellular and humoral immunity. Additionally, probiotics were added to reduce the side effects of radiation therapy for cervical malignancies[ 25 ].…”