Cis?trans isomerase gene in psychrophilic Pseudomonas syringae is constitutively expressed during growth and under conditions of temperature and solvent stress

Kiran, Madanahally D.; Annapoorni, S.; Suzuki, Iwane; Murata, Norio; Shivaji, Sisinthy

doi:10.1007/s00792-005-0435-6

Cited by 39 publications

(31 citation statements)

References 28 publications

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“…In contrast to our data, most studies to date have suggested that pseudomonal CTIs are constitutively expressed (47). However, resistance to hydrocarbons, in particular toluene, has previously been linked with the isomerization of cis to trans FAs through an upregulation of CTI (up to 1.4-fold) in P. putida (48).…”

Section: Figcontrasting

confidence: 99%

Acquisition and Role of Molybdate in Pseudomonas aeruginosa

Pederick

Eijkelkamp

Ween

et al. 2014

Appl Environ Microbiol

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In microaerophilic or anaerobic environments, Pseudomonas aeruginosa utilizes nitrate reduction for energy production, a process dependent on the availability of the oxyanionic form of molybdenum, molybdate (MoO 4 2؊ ). Here, we show that molybdate acquisition in P. aeruginosa occurs via a high-affinity ATP-binding cassette permease (ModABC). ModA is a cluster D-III solute binding protein capable of interacting with molybdate or tungstate oxyanions. Deletion of the modA gene reduces cellular molybdate concentrations and results in inhibition of anaerobic growth and nitrate reduction. Further, we show that conditions that permit nitrate reduction also cause inhibition of biofilm formation and an alteration in fatty acid composition of P. aeruginosa. Collectively, these data highlight the importance of molybdate for anaerobic growth of P. aeruginosa and reveal novel consequences of nitrate reduction on biofilm formation and cell membrane composition.

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Section: Figcontrasting

confidence: 99%

Acquisition and Role of Molybdate in Pseudomonas aeruginosa

Pederick

Eijkelkamp

Ween

et al. 2014

Appl Environ Microbiol

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“…P. syringae was mutagenized with a Tn5 transposon-based suicide plasmid vector (pOT182) (35,42), and cold-sensitive mutants were identified based on their inability to grow or their delayed growth on plates incubated at 4°C for 1 week. Growth characteristics were also analyzed using a UV-visible spectrophotometer (Shimadzu, Kyoto, Japan) (36,37).…”

Section: Generation Of Cold-sensitive Mutants Psychrophilic Pseudomomentioning

confidence: 99%

“…The disrupted gene was identified by inverse PCR (46) using 35 cycles and transposon-specific outward primers (primers Ash 1 and Ash 3 for upstream regions and primers Ash 1 and Ash 2 for downstream regions) ( Table 2). PCR-amplified products were purified and sequenced with a ABI Prism 3700 DNA sequencer (Applied Biosystems, California) (12,13,36,37) using Ash 1 and Ash 3.…”

Section: Generation Of Cold-sensitive Mutants Psychrophilic Pseudomomentioning

confidence: 99%

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Importance of trmE for Growth of the Psychrophile Pseudomonas syringae at Low Temperatures

Singh

Pindi

Dube

et al. 2009

Appl Environ Microbiol

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Transposon mutagenesis of Pseudomonas syringae Lz4W, a psychrophilic bacterium capable of growing at temperatures between 2 and 30°C, yielded 30 cold-sensitive mutants, and CSM1, one of these cold-sensitive mutants, was characterized. Growth of CSM1 was retarded when it was cultured at 4°C but not when it was cultured at 22°C and 28°C compared to the growth of wild-type cells, indicating that CSM1 is a cold-sensitive mutant of P. syringae Lz4W. The mutated gene in CSM1 was identified as trmE (coding for tRNA modification GTPase), and evidence is provided that this gene is induced at low temperatures. Further, the cold-inducible nature of the trmE promoter was demonstrated. In addition, the transcription start site and the various regulatory elements of the trmE promoter, such as the ؊10 region, ؊35 region, UP element, cold box, and DEAD box, were identified, and the importance of these regulatory elements in promoter activity were confirmed. The importance of trmE in rapid adaptation to growth at low temperatures was further highlighted by plasmid-mediated complementation that alleviated the cold-sensitive phenotype of CSM1.Psychrophilic bacteria (57) constitute a sizeable proportion of bacterial diversity because a large proportion of Earth's biosphere (75%) is either transiently or permanently cold (temperature, Ͻ5°C) (2). Studies have indicated that psychrophiles adapt to low temperatures by being able to sense changes in temperature (41,48,59), by modulating membrane fluidity (11-13, 28, 29), and because they possess enzymes and genes which are active at low temperatures (8,10,19,35,50,60,64). In psychrophilic bacteria pnp (encoding polynucleotide phosphorylase) (23), oppA (mediation of the transport of oligonucleotides) (5), and recD (51) have been identified as genes required for low-temperature growth. In contrast, in mesophilic bacteria many genes are induced following a downshift in temperature; these genes include genes for fatty acid desaturases and other enzymes (26,32,62), cold shock genes (33, 47), and genes involved in replication transcription and translation (3,9,26,33,69). The question is whether such genes are induced in psychrophiles, which, unlike mesophiles, are not cold stressed but are cold adapted. The present study investigated the role of trmE in low-temperature growth. MATERIALS AND METHODS Generation of cold-sensitive mutants. Psychrophilic Pseudomonas syringaeLz4W (referred to as P.syringae below) and Escherichia coli strains DH-5␣ and S-17-1 (Table 1) were grown in Antarctic bacterial medium (58) or Luria-Bertani medium (66). P. syringae was mutagenized with a Tn5 transposon-based suicide plasmid vector (pOT182) (35, 42), and cold-sensitive mutants were identified based on their inability to grow or their delayed growth on plates incubated at 4°C for 1 week. Growth characteristics were also analyzed using a UV-visible spectrophotometer (Shimadzu, Kyoto, Japan) (36, 37).Identification of the disrupted gene. Southern analysis using genomic DNA of CSM1 (one of the cold-sensitive mutan...

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“…Thus, the predominant response of most bacteria to environmental perturbations is the alteration of the saturated/ unsaturated fatty acid ratio (Cronan & Gelmann, 1975;Ingram, 1976;Härtig et al, 1999;Ramos et al, 2002;Mrozik et al, 2004;Bisbiroulas et al, 2011;Paulucci et al, 2011). Other mechanisms, such as isomerization of cis/ trans double bonds (Gillan et al, 1981;Okuyama et al, 1991;Diefenbach et al, 1992;Heipieper et al, 2003;Bernal et al, 2007b;Kiran et al, 2005), shortening or elongation of fatty acid chain lengths (Cronan & Gelmann, 1975;Russell, 1984;Diefenbach et al, 1992) and changes in the ratio of terminally branched iso/anteiso fatty acids (Kaneda, 1977;Konopásek et al, 2000), have been found to play a role in the fluidity balance within bacteria. Modifications in the polar heads of anionic phospholipids have also been associated with the response to certain types of stress.…”

Section: Introductionmentioning

confidence: 99%

Coordinated response of phospholipids and acyl components of membrane lipids in Pseudomonas putida A (ATCC 12633) under stress caused by cationic surfactants

et al. 2014

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The present study assessed the role of membrane components of Pseudomonas putida A (ATCC 12633) under chemical stress conditions originated by treatment with tetradecyltrimethylammonium bromide (TTAB), a cationic surfactant. We examined changes in fatty acid composition and in the fluidity of the membranes of cells exposed to TTAB at a specific point of growth as well as of cells growing with TTAB. The addition of 10-50 mg TTAB l "1 promoted an increase in the saturated/unsaturated fatty acid ratio. By using fluorescence polarization techniques, we found that TTAB exerted a fluidizing effect on P. putida A (ATCC 12633) membranes. However, a complete reversal of induced membrane fluidification was detected after 15 min of incubation with TTAB. Consistently, the proportion of unsaturated fatty acids was lower in TTAB-treated cells as compared with non-treated cells. In the presence of TTAB, the content of phosphatidylglycerol increased (120 %), whilst that of cardiolipin decreased (60 %). Analysis of the fatty acid composition of P. putida A (ATCC 12633) showed that phosphatidylglycerol carried the major proportion of saturated fatty acids (89 %), whilst cardiolipin carried an elevated proportion of unsaturated fatty acids (18 %). The increase in phosphatidylglycerol and consequently in saturated fatty acids, together with a decrease in cardiolipin content, enabled greater membrane resistance, reversing the fluidizing effect of TTAB. Therefore, results obtained in the present study point to changes in the fatty acid profile as an adaptive response of P. putida A (ATCC 12633) cells to stress caused by a cationic surfactant.

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Cis?trans isomerase gene in psychrophilic Pseudomonas syringae is constitutively expressed during growth and under conditions of temperature and solvent stress

Cited by 39 publications

References 28 publications

Acquisition and Role of Molybdate in Pseudomonas aeruginosa

Acquisition and Role of Molybdate in Pseudomonas aeruginosa

Importance of trmE for Growth of the Psychrophile Pseudomonas syringae at Low Temperatures

Coordinated response of phospholipids and acyl components of membrane lipids in Pseudomonas putida A (ATCC 12633) under stress caused by cationic surfactants

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