Cis- and trans-acting factors for transcription of the adenovirus 12 E1A gene

Shibata, Hitomi; Zheng, Jia‐Hua; Koikeda, Satoshi; Masamune, Yukito; Nakanishi, Yoshinobu

doi:10.1016/0167-4781(89)90037-7

Cited by 9 publications

(6 citation statements)

References 52 publications

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“…The nucleotide sequences of all fragments were t Present address: Diagen, Hilden, Germany. determined in an Applied Biosystems 373A sequencer by using appropriate 19to 24-nucleotide-pair primers and the chain termination method (37). The nucleotide sequence of both DNA strands was determined over the entire molecule (see the primer locations in Fig.…”

Section: Methodsmentioning

confidence: 99%

Nucleotide sequence of human adenovirus type 12 DNA: comparative functional analysis

Sprengel

Schmitz

Heuss-Neitzel

et al. 1994

J Virol

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A fresh inoculum of human adenovirus type 12 (Ad12) was obtained from the American Type Culture Collection and passaged once on human embryonic kidney cells, and Ad12 DNA was prepared from the first-passage yield to avoid higher passages which might have generated host-virus DNA recombinants. The 18 PstI fragments of Ad12 DNA were cloned into the pBluescript KS vector, and the entire nucleotide sequence of both strands from all 18 fragments was determined by using successive oligodeoxyribonucleotide primers. Ad12 DNA extends over 34,125 nucleotide pairs, and its molecular weight is calculated to be about 22 x 10(6). The nucleotide sequence of Ad12 DNA was subjected to computer analyses that determined possible open reading of frames on the two strands, the leader sequences, the position of the virus-associated RNA coding region, possible TATA, and polyadenylation signals. The distribution of the Ad12 open reading frames was similar to that in the previously sequenced Ad2 DNA, but there were also distinct differences. Ad12 DNA has an inverted terminal redundancy of 161 nucleotides, compared with 102 nucleotides in Ad2 DNA. There were stretches of sequence identity between Ad2 and Ad12 DNAs at both termini; the overall sequence similarity between the two viral genomes ranged between 59% (polypeptide IX) and 77% (in the E2 region), with high homology also in the sequences for the adenovirus DNA polymerase.

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Section: Methodsmentioning

confidence: 99%

Nucleotide sequence of human adenovirus type 12 DNA: comparative functional analysis

Sprengel

Schmitz

Heuss-Neitzel

et al. 1994

J Virol

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“…Thus, for Ad12 it was shown by in vitro transcription assays that the promoters of both TS1 and TS2 are individually stimulated by cellular factors Nakanishi et al, 1987). Previous reports have indicated that the binding of nuclear factor-I (NF-I) to a region located near the left end of the Ad12 genome is involved in stimulation of TS1 (Koikeda et al, 1990 ;Shibata et al, 1989) and that the DNA-binding activity of NF-I might be inhibited by the E1A protein (Koikeda et al, 1990 ;Shibata-Sakurai et al, 1991). In addition, the E1A-stimulating factor 1 (ESF-1) was found to activate basal transcription from TS2 by interacting with a palindromic DNA sequence immediately upstream of the TATA box (Shibata-Sakurai et al, 1991, 1993.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, Ad12 possesses two functional transcriptional start sites located at nt 306 (TS1) and 445 (TS2). Both initiation sites are active at an early stage after infection with virus (Saito et al, 1981 ;Sawada & Fujinaga, 1980), resulting in a long and a short 13S-and 12S-primary transcript (Fujinaga et al, 1984 ;Nakanishi et al, 1987 ;Shibata et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

A cis-acting element 7 bp upstream of the ESF-1-binding motif is involved in E1A 13S autoregulation of the adenovirus 12 TS2 promoter.

Pützer

Gnauck

Kirch

et al. 1997

Journal of General Virology

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“…Nuclear extracts were prepared from Ehrlich ascites tumor cells essentially by the procedure of Dignam et al (12). Cell-free transcription was done in a 25-,ul reaction mixture with 0.1 pmol of DNA templates and 10 ,ul of nuclear extracts, and transcripts were analyzed by primer extension as previously described (50,51). In primer extension, the CAT primer, 5'-CAACGGTGGTATATCCAGTG, was used for the Ad2 E3 gene, the human ,3-actin gene, and the proximal transcription of the Adl2 Ela gene, whereas the distal transcription of the Adl2 Ela gene was analyzed with the specific D primer (50).…”

Section: Methodsmentioning

confidence: 99%

Transcription stimulation of the adenovirus type 12 E1a gene in vitro by the 266-amino-acid E1A protein

Kawamura

Wada

Makino

et al. 1994

J Virol

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We previously showed that the 13S but not the 12S mRNA product of the Ela gene of the highly oncogenic type 12 adenovirus (Adl2) stimulates the expression of its own gene. In this study, the mechanism for the autoregulation of the Adl2 Ela gene was investigated in vitro. The 266-amino-acid ElA protein of Adl2 was synthesized in yeast cells and purified as a 57-kDa polypeptide. The purified Adl2 ElA protein stimulated transcription from the proximal promoter of its own gene but had almost no effect on that from the distal promoter. A 35-bp upstream region including a TATA box for the proximal promoter seemed to be sufficient for transcription stimulation by the EIA protein. The Adl2 ElA protein formed a complex with a TATA box-binding protein (TBP), as does the EIA protein of nononcogenic Ad serotypes. Moreover, the ElA protein significantly reduced the binding of TBP to a TATA sequence, while it did not affect the DNA-binding activity of nuclear factor I, a stimulatory protein of the distal transcription of the Adl2 Ela gene. These results suggest that the 13S mRNA product of the Adl2 Ela gene regulates the transcription of its own gene by modulating the activity of TBP.

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Cis- and trans-acting factors for transcription of the adenovirus 12 E1A gene

Cited by 9 publications

References 52 publications

Nucleotide sequence of human adenovirus type 12 DNA: comparative functional analysis

Nucleotide sequence of human adenovirus type 12 DNA: comparative functional analysis

A cis-acting element 7 bp upstream of the ESF-1-binding motif is involved in E1A 13S autoregulation of the adenovirus 12 TS2 promoter.

Transcription stimulation of the adenovirus type 12 E1a gene in vitro by the 266-amino-acid E1A protein

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