CircXPO1 Promotes Glioblastoma Malignancy by Sponging miR-7-5p

Wang, Xuehui; Wang, Jiaying; An, Zihui; Yang, Adeline; Qiu, Mengsheng; Tan, Zhou

doi:10.3390/cells12060831

Cited by 2 publications

(1 citation statement)

References 48 publications

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“…39 Besides, the results of the rescue experiments revealed that miR-488-3p inhibition or MEF2C upregulation reversed the inhibitory impact of circVCAN silencing on glioma cell growth as well as metastasis, implicating that circVCAN boosted the malignant behaviors of glioma cells through regulating the miR-488-3p/MEF2C axis, which was in line with previous reports. 40,41 MEF2C, a member of the myocyte enhancer factor 2 family, is a transcription factor essential for the development of organs such as heart 42 and nervous system. 43 The function of MEF2C in cancer is specific to the environment, serving as an activator of tumorigenesis in T-cell acute lymphoblastic leukemia, 44 driving chemotherapy resistance in hepatocellular carcinoma cells, 45 or inhibiting tumor growth in soft-tissue sarcomas.…”

Section: Discussionmentioning

confidence: 99%

CircVCAN promotes glioma progression through the miR‐488‐3p/MEF2C‐JAGGED1 axis

Yang,

Gao,

Dong

2024

Environmental Toxicology

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Gliomas are the most prevalent primary malignant brain tumors worldwide. Growing evidences indicate that circular RNAs (circRNAs) play an important role in the regulation of biological behavior of tumors. We aimed to investigate the role and mechanism of circVCAN in glioma. RNase R treatment was utilized to assess the cyclic properties of circVCAN. CircVCAN, miR‐488‐3p, and myocyte enhancer factor 2C (MEF2C) levels in glioma tissues and cells were detected by reverse transcription real‐time polymerase chain reaction (RT‐qPCR), and the localization of them in glioma cells was determined with fluorescence in situ hybridization. Furthermore, a variety of biologically functional assessments were used to validate the role of circVCAN in glioma. The regulatory mechanisms of circVCAN, miR‐488‐3p, and MEF2C were further confirmed by double luciferase reporter gene assay, RNA immunoprecipitation and RNA pull‐down assay, and the binding of MEF2C to JAGGED1 was revealed by chromatin immunoprecipitation. Additionally, a xenograft tumor model was constructed to demonstrate the effect of circVCAN on tumor growth in vivo. Our results indicated that circVCAN was more stable than its linear RNA and was significantly upregulated in gliomas. CircVCAN overexpression stimulated glioma cells to proliferate and metastasize, but circVCAN silencing exerted the opposite effect. Meanwhile, silencing circVCAN inhibited tumor growth in vivo. Moreover, we found that circVCAN interacted with miR‐488‐3p to regulate MEF2C expression, and miR‐488‐3p inhibition or MEF2C overexpression reversed the inhibitory effect on malignant bio‐behaviors mediated by circVCAN knockdown in glioma cells. MEF2C promoted the transcription of JAGGED1, and circVCAN knockdown reduced the binding between MEF2C and JAGGED1. Collectively, circVCAN is a carcinogenic circRNA in glioma, and the circVCAN/miR‐488‐3p/MEF2C‐JAGGED1 axis could serve as a potential target for the management of glioma.

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Section: Discussionmentioning

confidence: 99%

CircVCAN promotes glioma progression through the miR‐488‐3p/MEF2C‐JAGGED1 axis

Yang,

Gao,

Dong

2024

Environmental Toxicology

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The Glioblastoma CircularRNAome

Tirpe,

Streianu,

Tirpe

et al. 2023

IJMS

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Glioblastoma remains one of the most aggressive cancers of the brain, warranting new methods for early diagnosis and more efficient treatment options. Circular RNAs (circRNAs) are rather new entities with increased stability compared to their linear counterparts that interact with proteins and act as microRNA sponges, among other functions. Herein, we provide a critical overview of the recently described glioblastoma-related circRNAs in the literature, focusing on their roles on glioblastoma cancer cell proliferation, survival, migration, invasion and metastasis, metabolic reprogramming, and therapeutic resistance. The main roles of circRNAs in regulating cancer processes are due to their regulatory roles in essential oncogenic pathways, including MAPK, PI3K/AKT/mTOR, and Wnt, which are influenced by various circRNAs. The present work pictures the wide implication of circRNAs in glioblastoma, thus highlighting their potential as future biomarkers and therapeutic targets/agents.

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CircXPO1 Promotes Glioblastoma Malignancy by Sponging miR-7-5p

Cited by 2 publications

References 48 publications

CircVCAN promotes glioma progression through the miR‐488‐3p/MEF2C‐JAGGED1 axis

CircVCAN promotes glioma progression through the miR‐488‐3p/MEF2C‐JAGGED1 axis

The Glioblastoma CircularRNAome

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