CircWDR26 regulates endometrial carcinoma progression via miR-212-3p-mediated typing genes MSH2

Abstract

Background Circular RNAs (circRNA) are important in mediating tumor progression, but their roles in endometrial carcinoma (EC) are not fully understood yet. Many circRNAs are dysregulated and may contribute to EC progression. The functions of circWDR26 in EC remain unknown. Methods The expression of circWDR26 in EC and adjacent normal tissues, and cell lines was determined by qPCR. The proliferation, apoptosis, migration, and invasion of EC cells was examined by CCK-8 assay, flow cytometry, wound healing assay and Transwell assay. The interaction between circWDR26, MSH2 and miR-212-3p was determined by luciferase assay. EC cells were inoculated into nude mice and tumor burden was determined by measuring tumor dimensions, size, and weight. The proliferative marker Ki67 in EC tissue was determined by immunohistochemistry. Results The expression of circWDR26 in EC tissues or cell lines was higher than in the normal tissue or endometrial epithelial cells. Downregulation of circWDR26 resulted in attenuated proliferation, increased apoptosis, reduced migration and invasion of EC cells. Mechanistically, circWDR26 targeted and suppressed the expression of miR-212-3p. We further found that MSH2 was the novel target of miR-212-3p and was upregulated by circWDR26 via inhibiting miR-212-3p. In vivo experiment demonstrated that circWDR26 was essential for EC tumor growth. Conclusion circWDR26 promoted EC progression by regulating miR-212-3p/MSH2 axis and provided novel insights into anti-cancer treatment.

Dysregulated Expression Patterns of Circular RNAs in Cancer: Uncovering Molecular Mechanisms and Biomarker Potential

“…Functionally, hsa_circ_0002577 demonstrated roles in proliferation, migration, and invasion in vitro with effects on tumor growth in vivo, attributed to the sponging of miR-197 and downstream targeting of the CTNNC1/Wnt/β-catenin axis [129]. Confirma-tion of its upregulated expression in tumor samples and in vitro and in vivo phenotypic effects, further characterization of hsa_circ_0002577 demonstrated its role in accelerating progression of EC via activation of the IGF1R/PI3K/Akt signaling pathway through sponging of miR-625-5p [130]. This circRNA, also known as circWDR26, was evaluated in EC cells and demonstrated, in addition to the previously mentioned functions, inhibition of apoptosis.…”

Comprehensive analysis reveals the involvement of hsa_circ_0037858/miR-5000-3p/FMR1 axis in malignant metastasis of clear cell renal cell carcinoma

“…It has been widely acknowledged that miRNAs are involved in circRNA's biological effects [28][29][30]. Intriguingly, CSCD database [21] analysis revealed that hsa_circ_0037858 possessed potential microRNA response elements.…”

The m6A methyltransferase METTL3 drives thyroid cancer progression and lymph node metastasis by targeting LINC00894

“…Cell proliferation was assessed using the 5-ethynyl-2′-deoxyuridine (EdU) assay kit (RiboBio) according to the manufacturer’s guidelines [ 26 , 29 – 31 ]. Tumour cells were inoculated into 96-well plates(2 × 10 4 cells/well ) 24 h after transfection.…”