Circumferential stretching of saphenous vein smooth muscle enhances vasoconstrictor responses by Rho kinase-dependent pathways

McGregor, Emma; Gosling, Martin; Beattie, D.K.; Ribbons, Duncan M P; Davies, Alun H.; Powell, J.T.

doi:10.1016/s0008-6363(01)00436-9

Cited by 19 publications

(20 citation statements)

References 26 publications

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“…Samples from current smokers and patients with diabetes were excluded. Paired segments of vein were exposed to simulated venous or arterial flow in vitro for 90 min as described previously (4). Medial dissection was performed and validated by immunostaining for smooth muscle cell actin, smooth muscle cell myosin, and CD31 as described previously (6).…”

Section: Methodsmentioning

confidence: 99%

“…We report an almost 2-fold increase in total HSP27, with a decreased representation of the less acidic (phosphorylated) isoforms after hemodynamic stress. We speculate that this would permit more rapid agonist-induced phosphorylation of HSP27, to contribute to the enhanced phenylephrine-induced contractility of saphenous vein after exposure to hemodynamic stress (4).…”

Section: Capz (F-actin Capping Protein)-mentioning

confidence: 97%

“…Previously, we have shown that circumferential deformations, imposed by simulated arterial flow, enhance the contractility of venous smooth muscle (4). The enhanced force generation can be attenuated by low concentrations of cytochalasin B, a reagent that inhibits actin polymerization and attenuates the myogenic response.…”

Section: Capz (F-actin Capping Protein)-mentioning

confidence: 99%

“…modynamics. These changes include sensitization to vasoconstrictors and increased force generation, which involve Rho-kinase signaling (4). These changes in contractile properties are similar to the myogenic response, where changes in the polymerization of actin and the formation of contractile stress fibers in vascular smooth muscle cells have been proposed as downstream of Rho-kinase and other signaling pathways (5).…”

Section: Human Saphenous Vein (Hsv)mentioning

confidence: 99%

“…Organ Chamber Studies-Vein rings were mounted in organ chambers as previously described (4). Briefly, vein rings 5 mm in length were mounted in a 10-ml organ chamber suspended between two 0.2-mm steel wire stirrups, the upper one being attached to an isometric force transducer.…”

Section: Separation Of Proteins Using One-dimensional (1-d) Sds-page-12%mentioning

confidence: 99%

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F-actin Capping (CapZ) and Other Contractile Saphenous Vein Smooth Muscle Proteins Are Altered by Hemodynamic Stress

McGregor

Kempster

Wait³

et al. 2004

Molecular & Cellular Proteomics

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Increased force generation and smooth muscle remodeling follow the implantation of saphenous vein as an arterial bypass graft. Previously, we characterized and mapped 129 proteins in human saphenous vein medial smooth muscle using two-dimensional (2-D) PAGE and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Here, we focus on actin filament remodeling in response to simulated arterial flow. Human saphenous vein was exposed to simulated venous or arterial flow for 90 min in vitro, and the contractile medial smooth muscle was dissected out and subjected to 2-D gel electrophoresis using a non-linear immobilized pH 3-10 gradient in the first dimension. Proteins were analyzed quantitatively using PDQuest 2-D software. Human saphenous vein (HSV)1 remains a common conduit of choice for bypass grafting in humans because HSV is readily accessed, relatively plentiful, easily harvested (1), and provides adequate flow to the recipient artery. In the arterial bypass situation, the vessel experiences an abrupt change from the low-pressure, minimally pulsatile venous circulation to the high-pressure, pulsatile arterial circulation. The adaptation of HSV to the altered hemodynamic environment is a crucial process in graft maturation and patency.In the artery wall, smooth muscle cells are organized circumferentially and spirally, allowing efficient conduction of the arterial pulse. In contrast, the smooth muscle cells of saphenous vein are orientated both longitudinally and circumferentially. Re-orientation of the medial smooth muscle cells of saphenous vein into co-ordinated circumferential and spiral units is a crucial adaptive response observed in vein grafts. The thin-walled vein dilates in response to arterial pressure. Restoration of a smaller graft lumen with increase in wall thickness, to reduce wall tension, is achieved by migration of smooth muscle cells into the intima, with alteration to a synthetic, proliferative phenotype. This healing response of intimal hyperplasia can be locally excessive, particularly at the anastomoses, and is an important underlying cause of vein graft failure (2, 3). There are very early changes in the contractile properties of HSV following exposure to arterial heFrom the ‡Department

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Section: Methodsmentioning

confidence: 99%

Section: Capz (F-actin Capping Protein)-mentioning

confidence: 97%

Section: Capz (F-actin Capping Protein)-mentioning

confidence: 99%

Section: Human Saphenous Vein (Hsv)mentioning

confidence: 99%

Section: Separation Of Proteins Using One-dimensional (1-d) Sds-page-12%mentioning

confidence: 99%

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