Circumferential migration of ameboid microglia in the margin of the developing quail retina

Marín‐Teva, José L.; Calvente, Ruth; Cuadros, Miguel A.; Almendros, Antonio; Navascués, Julio

doi:10.1002/(sici)1098-1136(199909)27:3<226::aid-glia4>3.0.co;2-t

Cited by 26 publications

(16 citation statements)

References 41 publications

(46 reference statements)

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“…Macrophages and microglial cells are present in the developing and mature retina, and their distribution through the retinal layers has been well documented in vertebrates (Ashwell, 1989;Marín-Teva et al, 1998;Marín-Teva et al, 1999a;Marín-Teva et al, 1999b;Rodríguez-Gallardo et al, 2005;Santos et al, 2008;BejaranoEscobar et al, 2011). Abundant microglial cells, identified with nucleoside diphosphatase and tomato lectin histochemistry, can also be observed in the retina, optic nerve and optic tectum of adult tench (Velasco et al, 1995;Velasco et al, 1999).…”

Section: Microglia In the Experimental Tench Retinal Tissuementioning

confidence: 98%

Light-induced degeneration and microglial response in the retina of an epibenthonic pigmented teleost: age-dependent photoreceptor susceptibility to cell death

Bejarano-Escobar

Blasco²,

Martı́n-Partido

et al. 2012

Journal of Experimental Biology

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SUMMARYConstant intense light causes apoptosis of photoreceptors in the retina of albino fish. However, very few studies have been performed on pigmented species. Tench (Tinca tinca) is a teleost inhabiting dimly lit environments that has a predominance of rods within the photoreceptor layer. To test the hypothesis that constant high intensity light can result in retinal damage in such pigmented epibenthonic teleost species, photodegeneration of the retina was investigated in the larvae and in juveniles of tench to assess whether any damage may also be dependent on fish age. We exposed both groups of animals to 5days of constant darkness, followed by 4days of constant 20,000lx light, and then by 6days of recovery in a 14h light:10h dark cycle. The results showed that the retina of the larvae group exhibited abundant photoreceptor cell apoptosis during the time of exposition to intense light, whereas that of juveniles was indifferent to it. Damaged retinas showed a strong TUNEL signal in photoreceptor nuclei, and occasionally a weak cytoplasmic TUNEL signal in Müller glia. Specific labelling of microglial cells with Griffonia simplicifolia lectin (GSL) histochemistry revealed that photoreceptor cell death alerts microglia in the degenerating retina, leading to local proliferation, migration towards the injured outer nuclear layer (ONL), and enhanced phagocytosis of photoreceptor debris. During the first days of intense light treatment, Müller cells phagocytosed dead photoreceptor cells but, once microglial cells became activated, there was a progressive increase in the phagocytic capacity of the microglia.

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Section: Microglia In the Experimental Tench Retinal Tissuementioning

confidence: 98%

Light-induced degeneration and microglial response in the retina of an epibenthonic pigmented teleost: age-dependent photoreceptor susceptibility to cell death

Bejarano-Escobar

Blasco²,

Martı́n-Partido

et al. 2012

Journal of Experimental Biology

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“…Later in development, microglial precursors enter the differentiating retina by crossing the vitreal surface of the retina, from the optic nerve head, and by migration from nonneural ciliary regions after crossing the peripheral margin of the retina (Díaz‐Araya et al, ; Navascués et al, ; Marín‐Teva et al, 1999a, b; Santos et al, ; Bejarano‐Escobar et al, ). These specialized phagocytes colonize the visual system by different forms of migration.…”

Section: Phagocytosis Of Cell Debris During Ontogenymentioning

confidence: 99%

Ontogenetic Cell Death and Phagocytosis in the Visual System of Vertebrates

Francisco-Morcillo

Bejarano-Escobar

Rodríguez-León

et al. 2014

Developmental Dynamics

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Programmed cell death (PCD), together with cell proliferation, cell migration, and cell differentiation, is an essential process during development of the vertebrate nervous system. The visual system has been an excellent model on which to investigate the mechanisms involved in ontogenetic cell death. Several phases of PCD have been reported to occur during visual system ontogeny. During these phases, comparative analyses demonstrate that dying cells show similar but not identical spatiotemporally restricted patterns in different vertebrates. Additionally, the chronotopographical coincidence of PCD with the entry of specialized phagocytes in some regions of the developing vertebrate visual system suggests that factors released from degenerating cells are involved in the cell migration of macrophages and microglial cells. Contradicting this hypothesis however, in many cases the cell corpses generated during degeneration are rapidly phagocytosed by neighboring cells, such as neuroepithelial cells or M€ uller cells. In this review, we describe the occurrence and the sites of PCD during the morphogenesis and differentiation of the retina and optic pathways of different vertebrates, and discuss the possible relationship between PCD and phagocytes during ontogeny.

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“…Mü ller cells were identified by immunostaining with the monoclonal antibody H5 (DSHB), which recognizes vimentin, a component of the Mü ller cell cytoskeleton (Belmonte et al, 2000). Monoclonal antibodies C4 and M1B4 (both from DSHB) were used to label s-laminin and tenascin, respectively; s-laminin is an adhesion-related glycoprotein expressed in the retina by Mü ller cells (Libby et al, 1997;Marín-Teva et al, 1999b), and tenascin is an extracellular matrix protein expressed in the plexiform layers of the embryonic retina (Perez and Halfter, 1993;Belmonte et al, 2000).…”

Section: Immunocytochemistrymentioning

confidence: 99%

Radial migration of developing microglial cells in quail retina: A confocal microscopy study

Sánchez‐López

Cuadros

Calvente

et al. 2004

Glia

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Microglial cells spread within the nervous system by tangential and radial migration. The cellular mechanism of tangential migration of microglia has been described in the quail retina but the mechanism of their radial migration has not been studied. In this work, we clarify some aspects of this mechanism by analyzing morphological features of microglial cells at different steps of their radial migration in the quail retina. Microglial cells migrate in the vitreal half of the retina by successive jumps from the vitreal border to progressively more scleral levels located at the vitreal border, intermediate regions, and scleral border of the inner plexiform layer (IPL). The cellular mechanism used for each jump consists of the emission of a leading thin radial process that ramifies at a more scleral level before retraction of the rear of the cell. Hence, radial migration and ramification of microglial cells are simultaneous events. Once at the scleral border of the IPL, microglial cells migrate through the inner nuclear layer to the outer plexiform layer by another mechanism: they retract cell processes, become round, and squeeze through neuronal bodies. Microglial cells use radial processes of s-lamininexpressing Mü ller cells as substratum for radial migration. Levels where microglial cells stop and ramify at each jump are always interfaces between retinal strata with strong tenascin immunostaining and strata showing weak or no tenascin immunoreactivity. When microglial cell radial migration ends, tenascin immunostaining is no longer present in the retina. These findings suggest that tenascin plays a role in the stopping and ramification of radially migrating microglial cells.

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Circumferential migration of ameboid microglia in the margin of the developing quail retina

Cited by 26 publications

References 41 publications

Light-induced degeneration and microglial response in the retina of an epibenthonic pigmented teleost: age-dependent photoreceptor susceptibility to cell death

Light-induced degeneration and microglial response in the retina of an epibenthonic pigmented teleost: age-dependent photoreceptor susceptibility to cell death

Ontogenetic Cell Death and Phagocytosis in the Visual System of Vertebrates

Radial migration of developing microglial cells in quail retina: A confocal microscopy study

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