Circulatory miR-221 &amp; miR-542 expression profiles as potential molecular biomarkers in Hepatitis C Virus mediated liver cirrhosis and hepatocellular carcinoma

Yasser, Montaser Bellah; abdellatif, Mohamed; Emad, Esraa; Jafer, Afnan; Ahmed, Samar; Nageb, Lobna; Abdelshafy, Hanan; Al-Anany, Amany Mohamed; Al-Arab, Mohamed Ali Ezz; Gibriel, Abdullah Ahmed

doi:10.1016/j.virusres.2021.198341

Cited by 16 publications

(15 citation statements)

References 52 publications

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“…Significant changes in the ratio of vitamin intake were found to affect the CpG island methylation pattern in the loci of DNMT1, DNMT3A and DNMT3B, consequently leading to increased expression of the methyltransferases [ 29 ]. Even levels of miRNAs like that of miR-133 (linked to muscle and cardiac tissue metabolism and certain kinds of cancer) [ 54 , 58 , 65 , 85 , 86 , 98 , 101 ] and miR-221 (linked to oncogenesis) [ 10 , 25 , 50 , 56 , 60 , 71 , 79 , 90 , 96 , 100 ] changed in response to a skewed ratio of intake of the two vitamins. Other than sub or supra-optimal levels of folate or B12 intake, vitamin C or ascorbic acid consumption during the perinatal period also seems significant [ 19 ].…”

Section: Maternal Dietary and Supplementary Intakesmentioning

confidence: 99%

DNA methylation and regulation of gene expression: Guardian of our health

Dhar

Saha

Mitra

et al. 2021

Nucleus

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One of the most critical epigenetic signatures present in the genome of higher eukaryotes is the methylation of DNA at the C-5 position of the cytosine ring. Based on the sites of DNA methylation in a locus, it can serve as a repressive or activation mark for gene expression. In a crosstalk with histone modifiers, DNA methylation can consequently either inhibit binding of the transcription machinery or generate a landscape conducive for transcription. During developmental phases, the DNA methylation pattern in the genome undergoes alterations as a result of regulated balance between de novo DNA methylation and demethylation. Resultantly, differentiated cells inherit a unique DNA methylation pattern that fine tunes tissue-specific gene expression. Although apparently a stable epigenetic mark, DNA methylation is actually labile and is a complex reflection of interaction between epigenome, genome and environmental factors prior to birth and during progression of life. Recent findings indicate that levels of DNA methylation in an individual is a dynamic outcome, strongly influenced by the dietary environment during germ cell formation, embryogenesis and post birth exposures. Loss of balances in DNA methylation during developmental stages may result in imprinting disorders, while at any later stage may lead to increased predisposition to various diseases and abnormalities. This review aims to provide an outline of how our epigenome is uniquely guided by our lifetime of experiences beginning in the womb and how understanding it better holds future possibilities of improvised clinical applications.

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Section: Maternal Dietary and Supplementary Intakesmentioning

confidence: 99%

DNA methylation and regulation of gene expression: Guardian of our health

Dhar

Saha

Mitra

et al. 2021

Nucleus

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“…Liver cirrhosis is an advanced scar formation process. Due to long‐term liver injury, healthy liver tissue is replaced by nodules and scar tissue, and the scar tissue is surrounded by fiber bundles 4 . Irreversible liver cirrhosis may eventually develop into HCC.…”

Section: Introductionmentioning

confidence: 99%

“…Due to long‐term liver injury, healthy liver tissue is replaced by nodules and scar tissue, and the scar tissue is surrounded by fiber bundles. 4 Irreversible liver cirrhosis may eventually develop into HCC. Therefore, finding new targets for therapeutic and diagnostic methods will help in the early diagnosis and intervention of HCC.…”

Section: Introductionmentioning

confidence: 99%

LncRNA SLC16A1‐AS1 contributes to the progression of hepatocellular carcinoma cells by modulating miR‐411/MITD1 axis

Duan

2022

Clinical Laboratory Analysis

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Background Hepatocellular carcinoma (HCC) is a common malignancy with high morbidity. The current study aimed to explore the molecular mechanism of lncRNA SLC16A1‐AS1 in the tumorigenesis of HCC. Material and methods The expression of SLC16A1‐AS1 and miR‐411 was examined in clinical HCC tissues. HCC cell lines Hep3B and Huh‐7 were employed and transfected with si‐SLC16A1‐AS1. The correlation between SLC16A1‐AS1 and miR‐411 was verified by luciferase reporter assay. Cell viability was detected by CCK‐8 assay. Cell migration and invasion capacity were examined by transwell assay. The protein level of MITD1 was analyzed by western blotting. Results The expression of SLC16A1‐AS1 markedly increased in HCC tissues and cell lines. Subsequent studies identified SLC16A1‐AS1 as a downstream target of miR‐411. In addition, SLC16A1‐AS1 knockdown and miR‐411 overexpression significantly stagnated the progression of HCC cells. SLC16A1‐AS1 knockdown also downregulated MITD1 levels. Conclusion Our findings showed that SLC16A1‐AS1 was overexpressed in HCC cells and tissues. SLC16A1‐AS1 promoted the malignant characteristics of HCC cells and acted as an oncogene. Its regulatory effect may be associated with miR‐411/MITD1 axis. Therefore, SLC16A1‐AS1 has the potential to be used as a biomarker or therapeutic target for the treatment of HCC.

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“… 7 , 8 As a small-molecule RNA, is abnormally expressed in patients suffering from hepatitis C, participating in the occurrence and development of hepatitis C as a hidden target for hepatitis C treatment. 9 , 10 Multiple miRNAs are dysregulating and have been recognized for being potential and promising biomarkers in HCC diagnosis and staging, 11 , 12 including miR-30b, 13 miR-485, 14 miR-545, 15 miR-30a-5p, 16 miR-214, 17 miR-497-5p 18 and miR-9-5p. 19 According to current researches, miR-155 and miR-224 participate in mediating hepatocyte immune response, exert a significant effect on the progression and metastasis of HCC, and are expected to become potential markers for the diagnosis of HCC.…”

Section: Introductionmentioning

confidence: 99%

Expression and Clinical Values of Serum miR-155 and miR-224 in Chinese Patients with HCV Infection

X¹,

Zhang²,

Wang³

et al. 2022

IJGM

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Background To investigate the expression of serum miR-155 and miR-224 among patients with hepatitis C virus (HCV) infection and analyze their clinical values. Methods A total of 116 patients suffering from HCV infection admitted to our hospital and 70 healthy subjects were selected. According to the diagnostic results, patients with HCV infection were divided into 48 cases of chronic hepatitis C (CHC), 43 cases of liver cirrhosis and 25 cases of hepatocellular carcinoma (HCC). The expression signature for miR-155 and miR-224 was detected in serum samples. ROC curve and Pearson correlation test were conducted to investigate their diagnostic value and correlation. Results The expression extent for serum miR-155 and miR-224 increased along with the increase of malignancy (all P < 0.05). According to ROC curve, the area under the curve (0.918, 95% CI: 0.856–0.974) of miR-155 and miR-224 combined in the diagnosis of HCC was the largest, and its sensitivity and specificity were 93.0% and 86.2%. There is a positive relationship for expression level between miR-155 and miR-224 in CHC and HCC group (all P < 0.001). Conclusion miR-155 and miR-224 are remarkably increased in patients suffering from HCV infection. The combination of miR-155 and miR-224 has a good diagnostic value for HCC caused by HCV infection.

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Circulatory miR-221 & miR-542 expression profiles as potential molecular biomarkers in Hepatitis C Virus mediated liver cirrhosis and hepatocellular carcinoma

Cited by 16 publications

References 52 publications

DNA methylation and regulation of gene expression: Guardian of our health

DNA methylation and regulation of gene expression: Guardian of our health

LncRNA SLC16A1‐AS1 contributes to the progression of hepatocellular carcinoma cells by modulating miR‐411/MITD1 axis

Expression and Clinical Values of Serum miR-155 and miR-224 in Chinese Patients with HCV Infection

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