Circulatory exosomal tRF-Glu-CTC-005 and tRF-Gly-GCC-002 serve as predictive factors of successful microdissection testicular sperm extraction in patients with nonobstructive azoospermia

Zhang, Qiang; Zhao, Ling; Han, Xiaoxiao; Li, Ying; Xia, Tian; Zhu, Yehan; Li, Zhenbei; Wang, Liang; Lin, Hao; Hu, Fangfang; Cao, Yijuan; Han, Conghui; Zhu, Zuobin

doi:10.1016/j.fertnstert.2021.11.010

Cited by 8 publications

(6 citation statements)

References 33 publications

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“…Two studies have been identified in this review where tRNAs in EVs play potential roles in the diseases of the reproductive tract. Zhang et al [ 69 ] reported the use of exosomal tRFs to predict whether microdissection testicular sperm extraction (Micro-TESE) could be used to predict success as a sperm retrieval procedure for patients suffering from non-obstructive azoospermia (NOA). Differential expression of EV tRFs was analysed from the blood plasma of twelve patients with successful micro-TESE against eighteen patients where no sperm was retrieved.…”

Section: Resultsmentioning

confidence: 99%

The Clinical Significance of Transfer RNAs Present in Extracellular Vesicles

Liu

Yang

Asim

et al. 2022

IJMS

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Extracellular vesicles (EVs) are important for intercellular signalling in multi-cellular organisms. However, the role of mature transfer RNAs (tRNAs) and tRNA fragments in EVs has yet to be characterised. This systematic review aimed to identify up-to-date literature on tRNAs present within human EVs and explores their potential clinical significance in health and disease. A comprehensive and systematic literature search was performed, and the study was conducted in accordance with PRISMA guidelines. Electronic databases MEDLINE and EMBASE were searched up until 1 January 2022. From 685 papers, 60 studies were identified for analysis. The majority of papers reviewed focussed on the role of EV tRNAs in cancers (31.7%), with numerous other conditions represented. Blood and cell lines were the most common EV sources, representing 85.9% of protocols used. EV isolation methods included most known methods, precipitation being the most common (49.3%). The proportion of EV tRNAs was highly variable, ranging between 0.04% to >95% depending on tissue source. EV tRNAs are present in a multitude of sources and show promise as disease markers in breast cancer, gastrointestinal cancers, and other diseases. EV tRNA research is an emerging field, with increasing numbers of papers highlighting novel methodologies for tRNA and tRNA fragment discovery.

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Section: Resultsmentioning

confidence: 99%

The Clinical Significance of Transfer RNAs Present in Extracellular Vesicles

Liu

Yang

Asim

et al. 2022

IJMS

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“…57 In a recent study, Zhang et al identified the plasma extracellular vesicles and exosomes tRF-Gly-GCC-002 and tRF-Glu-CTC-005 as predictive markers of sperm retrieval. 58 Combining these markers with known variables may allow for a better model to predict sperm retrieval. Moreover, based on bioinformatics analysis and machine learning algorithms, including genomic differences, protein-protein interactions, and network analysis, NOA predictors include DDX20 and NCBP2, and single-cell RNA sequencing showed that these genes were significantly associated with spermatogenesis, leading to an artificial neural network diagnostic model.…”

Section: Future Perspectivesmentioning

confidence: 99%

“…In a recent study, Zhang et al. identified the plasma extracellular vesicles and exosomes tRF‐Gly‐GCC‐002 and tRF‐Glu‐CTC‐005 as predictive markers of sperm retrieval 58 . Combining these markers with known variables may allow for a better model to predict sperm retrieval.…”

Section: Future Perspectivesmentioning

confidence: 99%

Clinical management of nonobstructive azoospermia: An update

Takeshima,

Karibe,

Saito

et al. 2023

Int J of Urology

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Approximately 1% of the general male population has azoospermia, and nonobstructive azoospermia accounts for the majority of cases. The causes vary widely, including chromosomal and genetic abnormalities, varicocele, drug‐induced causes, and gonadotropin deficiency; however, the cause is often unknown. In azoospermia caused by hypogonadotropic hypogonadism, gonadotropin replacement therapy can be expected to produce sperm in the ejaculate. In some cases, upfront varicocelectomy for nonobstructive azoospermia with varicocele may result in the appearance of ejaculated spermatozoa; however, the appropriate indication should be selected. Each guideline recommends microdissection testicular sperm extraction for nonobstructive azoospermia in terms of successful sperm retrieval and avoidance of complications. Sperm retrieval rates generally ranged from 20% to 70% but vary depending on the causative disease. Various attempts have been made to predict sperm retrieval and improve sperm retrieval rates; however, the evidence is insufficient. Further evidence accumulation is needed for salvage treatment in cases of failed sperm retrieval. In Japan, there is inadequate provision on the right to know the origin of children born from artificial insemination of donated sperm and the rights of sperm donors, as well as information on unrelated family members, and the development of these systems is challenging. In the future, it is hoped that the pathogenesis of nonobstructive azoospermia with an unknown cause will be elucidated and that technology for omics technologies, human spermatogenesis using pluripotent cells, and organ culture methods will be developed.

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“…Moreover, the receiver operating characteristic area under the curve analysis presented these two tRFs as highly credible biomarkers for predicting SSR by micro-TESE in patients with INOA (Ref. 70). Exosome-carrying RNAs in seminal plasma have also been used to establish predictive models of the presence of testicular spermatozoa by micro-TESE in patients with INOA (Refs 71, 72).…”

Section: Rnasmentioning

confidence: 99%

Non-invasive molecular biomarkers for predicting outcomes of micro-TESE in patients with idiopathic non-obstructive azoospermia

Tang

et al. 2022

Expert Rev. Mol. Med.

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Non-obstructive azoospermia (NOA), the most severe type of male infertility, affects approximately 1% of men worldwide. However, the aetiology of most NOA cases is not definite, that is defined as idiopathic NOA (INOA), posing a clinical conundrum worldwide. Most of these patients must receive donor sperm treatment until the emergence of microdissection testicular sperm extraction (micro-TESE). Although this procedure has recently become a promising treatment for INOA, the low sperm retrieval rate and testicular trauma have prompted us to explore appropriate non-invasive molecular biomarkers to predict the outcomes of sperm recovery preoperatively. Previous studies have identified a spectrum of biomarkers to address this challenging issue at various levels in different tissues, such as DNAs, RNAs, protein and steroid levels in the blood and seminal fluid. To better understand and assess the predictive values of diverse molecular biomarkers from different tissues on the outcome of sperm retrieval by micro-TESE in patients with INOA, we summarised recent findings and discussed the potential applications of these methods. The ultimate goal of this study was to provide references for further studies and clinical management.

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Circulatory exosomal tRF-Glu-CTC-005 and tRF-Gly-GCC-002 serve as predictive factors of successful microdissection testicular sperm extraction in patients with nonobstructive azoospermia

Cited by 8 publications

References 33 publications

The Clinical Significance of Transfer RNAs Present in Extracellular Vesicles

The Clinical Significance of Transfer RNAs Present in Extracellular Vesicles

Clinical management of nonobstructive azoospermia: An update

Non-invasive molecular biomarkers for predicting outcomes of micro-TESE in patients with idiopathic non-obstructive azoospermia

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