Circulation of HAdV-41 with diverse genome types and recombination in acute gastroenteritis among children in Shanghai

Abstract

Human adenovirus F (HAdV-F) is one of the major causative species detected in acute gastroenteritis in children worldwide. HAdV-F is composed of serotypes 40 and 41. Most studies have reported the prevalence of HAdV-41 and focused on its epidemiologic characteristics. In this study, seventeen samples were identified as HAdV-41 out of 273 fecal specimens from children with acute diarrhea in Shanghai. Five isolates were isolated and subjected to whole genome sequencing and analysis to characterize the genetic variation and evolution. Full genome analysis revealed low genetic variation (99.07–99.92% identity) among the isolates, and InDels are observed in the E2A gene and the hexon gene compared to the reference strain NIVD103. Phylogenetic analysis showed that all isolates mainly formed two genome-type clusters but with incongruence in the trees of whole genomes and individual genes. The recombination breakpoints of the five isolates were inferred by the Recombination Detection Program (RDP) and varied in the number and location of the recombination events, indicating different evolution origins. Overall, our study highlights the genetic diversity of HAdV-41 isolates circulating in Shanghai, which may have evolved from inter-strain recombination.

Molecular and epidemiological trends of human bocavirus and adenovirus in children with acute gastroenteritis in Bangladesh during 2015 to 2019

“…In this study, most of the infants and children with AdV infection (64%) were aged less than 24 months. This finding was similar to the studies on AdV infection associated with acute gastroenteritis occurred predominantly in infants and young children worldwide 31,33,37‐40 …”

“…This finding was similar to the studies on AdV infection associated with acute gastroenteritis occurred predominantly in infants and young children worldwide. 31,33,[37][38][39][40] The most common signs and symptoms of infants and children infected with AdVs were diarrhea (93%), vomiting (57%), fever (50%), abdominal pain (42%) and dehydration (14.3%) which was in agreement with the previous reports of AdV-infected patients. 31 The study AdV strains had many substitution mutations at positions 20 to 40 and 105 to 145.…”

“…31,32 Epidemiological research detected AdVs in stool samples collected from infants and young children with acute gastroenteritis, in the developed and developing world. [31][32][33] Out of 290 fecal specimens tested, 14 (4.82%) were positive for AdVs. This prevalence was slightly higher than the previous findings in South Asia and Asia Pacific regions in which its prevalence was around 0.8% to 3.4%.…”

“…Molecular epidemiological research is increasingly popular in the field of clinical AdV research 31,32 . Epidemiological research detected AdVs in stool samples collected from infants and young children with acute gastroenteritis, in the developed and developing world 31‐33 …”

Viral gastroenteritis in Tocantins, Brazil: characterizing the diversity of human adenovirus F through next-generation sequencing and bioinformatics

“…The existence of some temporal order on genetic HAdV-F sequences was not observed. Although an increasing prevalence of HAdV-F41 was reported in acute gastroenteritis [27,29,91], only a few complete genomes of HAdV-F41 are currently available in the GenBank database. These are the first near full-length genomes sequences of HAdV-F40 and 41 acquired from Brazil.…”