Circulation of Asian-I and Cosmopolitan genotypes of Dengue-2 virus in northeast India, 2016–2017

Ujwal, S; Sabeena, Sasidharanpillai; Bhaskar, R. Kumar; D'Souza, G. F.; Santhosha, D; Auti, Amogh Milind; Kumar, Rajendra; Ramachandran, Sumathi; Maity, Hindol; Bhandari, Yuvraj; Jazeel, A; Arunkumar, G.

doi:10.4103/0972-9062.289402

Cited by 3 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cosmopolitan genotype comprised of two distinct groups. One group included sequences belonging to the Indian sub-continent lineage, which has largely been reported in the Indian sub-continent 66 , 67 . The other group included the rest of cosmopolitan genotype sequences reported from other countries, especially in Southeast Asia.…”

Section: Resultsmentioning

confidence: 99%

Evolution, heterogeneity and global dispersal of cosmopolitan genotype of Dengue virus type 2

Yenamandra

Koo

Chiang

et al. 2021

Sci Rep

View full text Add to dashboard Cite

Dengue virus type 2 (DENV-2) contributes substantially to the dengue burden and dengue-related mortality in the tropics and sub-tropics. DENV-2 includes six genotypes, among which cosmopolitan genotype is the most widespread. The present study investigated the evolution, intra-genotype heterogeneity and dispersal of cosmopolitan genotype to understand unique genetic characteristics that have shaped the molecular epidemiology and distribution of cosmopolitan lineages. The spatial analysis demonstrated a wide geo-distribution of cosmopolitan genotype through an extensive inter-continental network, anchored in Southeast Asia and Indian sub-continent. Intra-genotype analyses using 3367 envelope gene sequences revealed six distinct lineages within the cosmopolitan genotype, namely the Indian sub-continent lineage and five other lineages. Indian sub-continent lineage was the most diverged among six lineages and has almost reached the nucleotide divergence threshold of 6% within E gene to qualify as a separate genotype. Genome wide amino acid signatures and selection pressure analyses further suggested differences in evolutionary characteristics between the Indian sub-continent lineage and other lineages. The present study narrates a comprehensive genomic analysis of cosmopolitan genotype and presents notable genetic characteristics that occurred during its evolution and global expansion. Whether those characteristics conferred a fitness advantage to cosmopolitan genotype in different geographies warrant further investigations.

show abstract

Section: Resultsmentioning

confidence: 99%

Evolution, heterogeneity and global dispersal of cosmopolitan genotype of Dengue virus type 2

Yenamandra

Koo

Chiang

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…A total of 30 samples from our study along with 46 GenBank sequences from Bangladesh (33 samples from 2017, 12 samples from 2004 to 2011, and 1 sample from 2019) were used to produce a phylogenetic tree. The recent emergence of DENV2 cosmopolitan clade C has also been reported in northeastern India, Thailand, and China [ 34 , 56 , 57 ]. It would be interesting to compare the in vitro growth characteristics of the clade B virus with those of the clade C virus to determine why clade B viruses disappeared from Bangladesh.…”

Section: Discussionmentioning

confidence: 99%

Genetic Analysis of Dengue Virus in Severe and Non-Severe Cases in Dhaka, Bangladesh, in 2018–2022

Rahim

Hasan

Phadungsombat

et al. 2023

Viruses

View full text Add to dashboard Cite

Dengue virus (DENV) infections have unpredictable clinical outcomes, ranging from asymptomatic or minor febrile illness to severe and fatal disease. The severity of dengue infection is at least partly related to the replacement of circulating DENV serotypes and/or genotypes. To describe clinical profiles of patients and the viral sequence diversity corresponding to non-severe and severe cases, we collected patient samples from 2018 to 2022 at Evercare Hospital Dhaka, Bangladesh. Serotyping of 495 cases and sequencing of 179 cases showed that the dominant serotype of DENV shifted from DENV2 in 2017 and 2018 to DENV3 in 2019. DENV3 persisted as the only representative serotype until 2022. Co-circulation of clades B and C of the DENV2 cosmopolitan genotype in 2017 was replaced by circulation of clade C alone in 2018 with all clones disappearing thereafter. DENV3 genotype I was first detected in 2017 and was the only genotype in circulation until 2022. We observed a high incidence of severe cases in 2019 when the DENV3 genotype I became the only virus in circulation. Phylogenetic analysis revealed clusters of severe cases in several different subclades of DENV3 genotype I. Thus, these serotype and genotype changes in DENV may explain the large dengue outbreaks and increased severity of the disease in 2019.

show abstract

Instability of the NS1 Glycoprotein from La Reunion 2018 Dengue 2 Virus (Cosmopolitan-1 Genotype) in Huh7 Cells Is Due to Lysine Residues on Positions 272 and 324

Ogire

Diaz

Vidalain

et al. 2021

IJMS

View full text Add to dashboard Cite

La Reunion island in the South West Indian Ocean is now endemic for dengue following the introduction of dengue virus serotype 2 (DENV-2) cosmopolitan-I genotype in 2017. DENV-2 infection causes a wide spectrum of clinical manifestations ranging from flu-like disease to severe dengue. The nonstructural glycoprotein 1 (NS1) has been identified as playing a key role in dengue disease severity. The intracellular NS1 exists as a homodimer, whereas a fraction is driven towards the plasma membrane or released as a soluble hexameric protein. Here, we characterized the NS1 glycoproteins from clinical isolates DES-14 and RUN-18 that were collected during the DENV-2 epidemics in Tanzania in 2014 and La Reunion island in 2018, respectively. In relation to hepatotropism of the DENV, expression of recombinant DES-14 NS1 and RUN-18 NS1 glycoproteins was compared in human hepatoma Huh7 cells. We observed that RUN-18 NS1 was poorly stable in Huh7 cells compared to DES-14 NS1. The instability of RUN-18 NS1 leading to a low level of NS1 secretion mostly relates to lysine residues on positions 272 and 324. Our data raise the issue of the consequences of a defect in NS1 stability in human hepatocytes in relation to the major role of NS1 in the pathogenesis of the DENV-2 infection.

show abstract

Circulation of Asian-I and Cosmopolitan genotypes of Dengue-2 virus in northeast India, 2016–2017

Cited by 3 publications

References 0 publications

Evolution, heterogeneity and global dispersal of cosmopolitan genotype of Dengue virus type 2

Evolution, heterogeneity and global dispersal of cosmopolitan genotype of Dengue virus type 2

Genetic Analysis of Dengue Virus in Severe and Non-Severe Cases in Dhaka, Bangladesh, in 2018–2022

Instability of the NS1 Glycoprotein from La Reunion 2018 Dengue 2 Virus (Cosmopolitan-1 Genotype) in Huh7 Cells Is Due to Lysine Residues on Positions 272 and 324

Contact Info

Product

Resources

About