Circulation and biodistribution profiles of long-circulating PEG-liposomes of various sizes in rabbits

Awasthi, Vibhudutta; Garcia, Delia M.; Goins, Beth; Phillips, William T.

doi:10.1016/s0378-5173(02)00703-2

Cited by 195 publications

(168 citation statements)

References 31 publications

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“…administration, believed to result from rapid uptake by the reticuloendothelial system (RES) (25). Grafting liposomes with polyethylene glycol (PEG) has been shown to prolong liposome circulation in vivo (26)(27)(28). The presence of PEG attracts water to the liposome surface and provides a barrier against opsonins and cells of the RES (29).…”

Section: Introductionmentioning

confidence: 99%

PEGylation of a Factor VIII–Phosphatidylinositol Complex: Pharmacokinetics and Immunogenicity in Hemophilia A Mice

Peng

Kosloski

Nakamura

et al. 2011

AAPS J

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Abstract. Hemophilia A is an X-linked bleeding disorder caused by the deficiency of factor VIII (FVIII). Exogenous FVIII is administered therapeutically, and due to a short half-life, frequent infusions are often required. Fifteen to thirty-five percent of severe hemophilia A patients develop inhibitory antibodies toward FVIII that complicate clinical management of the disease. Previously, we used phosphatidylinositol (PI) containing lipidic nanoparticles to improve the therapeutic efficacy of recombinant FVIII by reducing immunogenicity and prolonging the circulating half-life. The objective of this study is to investigate further improvements in the FVIII-PI formulation resulting from the addition of polyethylene glycol (PEG) to the particle. PEGylation was achieved by passive transfer of PEG conjugated lipid into the FVIII-PI complex. PEGylated FVIII-PI (FVIII-PI/PEG) was generated with high association efficiency. Reduced activity in vitro and improved retention of activity in the presence of antibodies suggested strong shielding of FVIII by the particle; thus, in vivo studies were conducted in hemophilia A mice. Following intravenous administration, the apparent terminal half-life was improved versus both free FVIII and FVIII-PI, but exposure determined by area under the curve was reduced. The formation of inhibitory antibodies after subcutaneous immunization with FVIII-PI/PEG was lower than free FVIII but resulted in a significant increase in inhibitors following intravenous administration. Passive transfer of PEG onto the FVIII-PI complex does not provide any therapeutic benefit.

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Section: Introductionmentioning

confidence: 99%

PEGylation of a Factor VIII–Phosphatidylinositol Complex: Pharmacokinetics and Immunogenicity in Hemophilia A Mice

Peng

Kosloski

Nakamura

et al. 2011

AAPS J

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“…13 The particle size is small enough (e200 nm) to evade rapid sequestration by phagocytotic cells of the spleen and to allow long blood circulation. 14 As described in the Supporting Information (SI), 4-carboxyphenylboronic acid (0.15 g), N-hydroxysuccinimide (0.10 g), and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (0.2 g) were introduced to AP-MSN (400 mg) in DMSO (20 mL) to yield the boronic acid-functionalized (0.5 mmol g -1 ) MSN material (BA-MSN) (Figure 1b). The presence of both aminopropyl and phenylboronic acid groups stabilizes the formation of borates with 1,2-or 1,3-diols ( Figure 1a).…”

mentioning

confidence: 99%

Mesoporous Silica Nanoparticle-Based Double Drug Delivery System for Glucose-Responsive Controlled Release of Insulin and Cyclic AMP

et al. 2009

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A boronic acid-functionalized mesoporous silica nanoparticle-based drug delivery system (BA-MSN) for glucose-responsive controlled release of both insulin and cyclic adenosine monophosphate (cAMP) was synthesized. Fluorescein isothiocyanate-labeled, gluconic acid-modified insulin (FITC-G-Ins) proteins were immobilized on the exterior surface of BA-MSN and also served as caps to encapsulate cAMP molecules inside the mesopores of BA-MSN. The release of both G-Ins and cAMP was triggered by the introduction of saccharides. The selectivity of FITC-G-Ins release toward a series of carbohydrate triggers was determined to be fructose > glucose > other saccharides. The unique feature of this double-release system is that the decrease of FITC-G-Ins release with cycles can be balanced by the release of cAMP from mesopores of MSN, which is regulated by the gatekeeper effect of FITC-G-Ins. In vitro controlled release of cAMP was studied at two pH conditions (pH 7.4 and 8.5). Furthermore, the cytotoxicity of cAMP-loaded G-Ins-MSN with four different cell lines was investigated by cell viability and proliferation studies. The cellular uptake properties of cAMP-loaded FITC-BA-MSN with and without G-Ins capping were investigated by flow cytometry and fluorescence confocal microscopy. We envision that this glucose-responsive MSN-based double-release system could lead to a new generation of self-regulated insulin-releasing devices.

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“…Moreover, in the inflamed tissue the blood vessel presents endothelial junctions allowing the escape of the particles smaller that 200 nm from the blood circulation (Crommelin et al, 1999). (Awasthi et al, 2003;Crommelin et al, 1999;Erdogan et al, 2000;Boerman et al, 1997;Litzinger et al, 1994). In contrast, large liposomes are not retained in infectious sites since that they are rapidly cleared from circulation by the organs of the mononuclear phagocyte system (Oyen et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Technetium-99m-labeled stealth pH-sensitive liposomes: a new strategy to identify infection in experimental model

Carmo

Oliveira

Mota

et al. 2007

Braz. arch. biol. technol.

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Circulation and biodistribution profiles of long-circulating PEG-liposomes of various sizes in rabbits

Cited by 195 publications

References 31 publications

PEGylation of a Factor VIII–Phosphatidylinositol Complex: Pharmacokinetics and Immunogenicity in Hemophilia A Mice

PEGylation of a Factor VIII–Phosphatidylinositol Complex: Pharmacokinetics and Immunogenicity in Hemophilia A Mice

Mesoporous Silica Nanoparticle-Based Double Drug Delivery System for Glucose-Responsive Controlled Release of Insulin and Cyclic AMP

Technetium-99m-labeled stealth pH-sensitive liposomes: a new strategy to identify infection in experimental model

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