Circulating Tumour Cells in Predictive Molecular Pathology: Focus on Drug-Sensitive Assays and 3D Culture

Pallante, Pierlorenzo; Pisapia, Pasquale; Bellevicine, Claudio; Malapelle, Umberto; Troncone, Giancarlo

doi:10.1159/000496213

Cited by 5 publications

(3 citation statements)

References 121 publications

(174 reference statements)

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“…Essentially, this assay is based on the singular propension of tumor cells to disrupt and ingest collagen adhesion matrix fragments, providing information about cancer cells invasiveness [ 54 ] . On the other hand, the detection of proteins secreted, released or shed by viable cancer cells, is the core of the EPithelial ImmunoSPOT (EPISPOT) assay [ 55 ] . These types of techniques enable in vitro CTC expansions since they well preserve cell viability [ 12 , 17 ] [ Table 1 ].…”

Section: Ctc Cultures: a Strenuous Challengementioning

confidence: 99%

In vitro cultures of circulating tumor cells: a potential tool to unravel drug sensitivity

Renzi¹,

Marco²,

Meo³

et al. 2022

CDR

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Since taking part as leading actors in driving the metastatic process, circulating tumor cells (CTCs) have displayed a wide range of potential applications in the cancer-related research field. Besides their well-proved prognostic value, the role of CTCs in both predictive and diagnostics terms might be extremely informative about cancer properties and therefore highly helpful in the clinical decision-making process. Unfortunately, CTCs are scarcely released in the blood circulation and their counts vary a lot among different types of cancer, therefore CTC detection and consequent characterization are still highly challenging. In this context, in vitro CTC cultures could potentially offer a great opportunity to expand the number of tumor cells isolated at different stages of the disease and thus simplify the analysis of their biological and molecular features, allowing a deeper comprehension of the nature of neoplastic diseases. The aim of this review is to highlight the main attempts to establish in vitro CTC cultures from patients harboring different tumor types in order to highlight how powerful this practice could be, especially in optimizing the therapeutic strategies available in clinical practice and potentially preventing or contrasting the development of treatment resistance.

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Section: Ctc Cultures: a Strenuous Challengementioning

confidence: 99%

In vitro cultures of circulating tumor cells: a potential tool to unravel drug sensitivity

Renzi¹,

Marco²,

Meo³

et al. 2022

CDR

View full text Add to dashboard Cite

show abstract

“…For instance, the immunocytochemistry of CTCs permits CTC morphological analysis and labeling of specific ligands [129,130], whereas qRT-PCR allows the detection of specific biomarkers with high sensitivity [131]. Post-functional assays, where enriched CTCs are cultured in 2D or 3D models, also enable the evaluation of migration and invasion abilities [132,133]. The advancement in NGS technologies even granted the possibility of dissecting CTC at the single-cell level [134].…”

Section: Importance Of Enrichment Technique Over Selection Of Ctc Analysis and Characterization In The Crc Screening Stagementioning

confidence: 99%

Liquid Biopsy-Based Colorectal Cancer Screening via Surface Markers of Circulating Tumor Cells

et al. 2021

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Colorectal cancer (CRC) is ranked second for cancer-related deaths worldwide with approximately half of the patients being diagnosed at the late stages. The untimely detection of CRC results in advancement to the metastatic stage and nearly 90% of cancer-related deaths. The early detection of CRC is crucial to decrease its overall incidence and mortality rates. The recent introduction of circulating tumor cells (CTCs) has enabled a less invasive sampling method from liquid biopsies, besides revealing key information toward CRC metastasis. The current gold standard for CTC identification is the CellSearch® system (Veridex). This first-generation instrumentation relies on a single cell surface marker (CSM) to capture and count CTCs. Detection of CTCs allows the identification of patients at risk for metastasis, whereas CTC enumeration could improve risk assessment, monitoring of systemic therapy, and detection of therapy resistance in advanced metastatic CRC. In this review, we compared the pros and cons between single CSM-based CTC enrichment techniques and multi-marker-based systems. We also highlighted the challenges faced in the routine implementation of CSM-dependent CTC detection methods in CRC screening, prediction, prognosis, disease monitoring, and therapy selection toward precision medicine, as well as the dwelling on post-CTC analysis and characterization methods.

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“… 11 Biomarkers such as the presence of CTCs have been the subject of significant study, with a well-understood biological mechanism linking their presence to the risk of metastasis in a variety of cancers. 12,13 A quantitative imaging biomarker (QIB) is one which is extracted specifically from a quantitative imaging technique, i.e . one where it is possible to quantitatively measure properties from a given image.…”

Section: Introductionmentioning

confidence: 99%

Clinical quantitative MRI and the need for metrology

Cashmore

McCann²,

Wastling

et al. 2021

The British Journal of Radiology

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MRI has been an essential diagnostic tool in healthcare for several decades. It offers unique insights into most tissues without the need for ionising radiation. Historically, MRI has been predominantly used qualitatively, images are formed to allow visual discrimination of tissues types and pathologies, rather than providing quantitative measurements. Increasingly, quantitative MRI (qMRI) is also finding clinical application, where images provide the basis for physical measurements of, e.g. tissue volume measures and represent aspects of tissue composition and microstructure. This article reviews some common current research and clinical applications of qMRI from the perspective of measurement science. qMRI not only offers additional information for radiologists, but also the opportunity for improved harmonisation and calibration between scanners and as such it is well-suited to large-scale investigations such as clinical trials and longitudinal studies. Realising these benefits, however, presents a new kind of technical challenge to MRI practioners. When measuring a parameter quantitatively, it is crucial that the reliability and reproducibility of the technique are well understood. Strictly speaking, a numerical result of a measurement is meaningless unless it is accompanied by a description of the associated measurement uncertainty. It is therefore necessary to produce not just estimates of physical properties in a quantitative image, but also their associated uncertainties. As the process of determining a physical property from the raw MR signal is complicated and multistep, estimation of uncertainty is challenging and there are many aspects of the MRI process that require validation. With the clinical implementation of qMRI techniques and its continued expansion, there is a clear and urgent need for metrology in this field.

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Circulating Tumour Cells in Predictive Molecular Pathology: Focus on Drug-Sensitive Assays and 3D Culture

Cited by 5 publications

References 121 publications

In vitro cultures of circulating tumor cells: a potential tool to unravel drug sensitivity

In vitro cultures of circulating tumor cells: a potential tool to unravel drug sensitivity

Liquid Biopsy-Based Colorectal Cancer Screening via Surface Markers of Circulating Tumor Cells

Clinical quantitative MRI and the need for metrology

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