Circulating Tumour Cells in locally advanced head and neck cancer: Preliminary report about their possible role in predicting response to non-surgical treatment and survival

Buglione, Michela; Grisanti, Salvatore; Almici, Camillo; Mangoni, Monica; Polli, Caterina; Consoli, Francesca; Verardi, Rosanna; Costa, Loredana; Paiar, Fabiola; Pasinetti, Nadia; Bolzoni, Andrea; Marini, Mirella; Simoncini, Edda; Nicolai, Piero; Biti, Gianpaolo; Magrini, Stefano Maria

doi:10.1016/j.ejca.2012.05.007

Cited by 87 publications

(90 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Interestingly, absence or disappearance of CTCs during therapy was shown to be associated with partial or complete response to therapy (29). Furthermore, similar to our findings, CTCs were more frequently detected in patients with advancedstaged tumors (T4 vs. T1-3; 29) and positivity rates were comparable between these and our studies.…”

Section: Discussionsupporting

confidence: 81%

“…Data about CTC detection with the CellSearch system in patients with head and neck cancer have already been published; however, patient cohorts were heterogeneous with SCCs of different subanatomical regions, including hypo-and oropharyngeal cancer (29,30). Interestingly, absence or disappearance of CTCs during therapy was shown to be associated with partial or complete response to therapy (29).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Prognostic Relevance of Circulating Tumor Cells in Blood and Disseminated Tumor Cells in Bone Marrow of Patients with Squamous Cell Carcinoma of the Oral Cavity

Gröbe

Blessmann

Hanken

et al. 2014

Clinical Cancer Research

116

View full text Add to dashboard Cite

Purpose: Current staging methods for squamous cell carcinomas (SCC) of the oral cavity (OSCC) need to be improved to predict the risk of individual patients. Because hematogenous tumor cell dissemination is a key event in tumor progression, we assessed the prognostic significance of disseminated tumor cells (DTC) in bone marrow and circulating tumor cells (CTC) in peripheral blood from patients with OSCC.Experimental Design: From 110 patients with OSCC, tumors were surgically resected (R0) without neoadjuvant therapy. The CellSearch system was used to enumerate CTCs. Bone marrow was aspirated from the iliac crest, and mononuclear cells (MNC) were enriched by Ficoll density gradient centrifugation. To detect DTCs, MNCs were immunostained with the pan-keratin antibody A45-B/B3. Results were correlated with clinicopathologic parameters and clinical outcome such as recurrence and death during follow-up time (mean 916 days).Results: Ten of 80 patients (12.5%) harbored CTCs in peripheral blood, whereas in 18 of 90 patients (20.0%) DTCs in bone marrow could be detected. Surprisingly, in only 2 patients (1.8%) CTCs and DTCs were detected simultaneously. Significant correlations could be found for CTCs and tumor size (P ¼ 0.04), nodal status and DTCs (P ¼ 0.02), and distant metastasis with CTCs (P ¼ 0.004) and DTCs (P ¼ 0.005). Univariate and multivariate analyses revealed that CTCs and DTCs were significant and independent predictors of recurrence-free survival (P < 0.001).Conclusions: Both DTCs and CTCs are independent prognostic markers in patients with OSCC, predicting relapse with higher sensitivity at various disease stages than routine staging procedures. Bone marrow might be an interesting target organ for future therapeutic interventions. Clin Cancer Res; 20(2); 425-33. Ó2013 AACR.

show abstract

Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Prognostic Relevance of Circulating Tumor Cells in Blood and Disseminated Tumor Cells in Bone Marrow of Patients with Squamous Cell Carcinoma of the Oral Cavity

Gröbe

Blessmann

Hanken

et al. 2014

Clinical Cancer Research

116

View full text Add to dashboard Cite

show abstract

“…Recent research suggests a possible role of circulating tumor cells (CTCs) in determining different types of cancer [90]. These CTCs have been used to identify molecular alterations of PIK3CA and PTEN in patients with SCCHN.…”

Section: Future Clinical Perspectivesmentioning

confidence: 99%

Targeting the PI3K/AKT/mTOR pathway in squamous cell carcinoma of the head and neck

2015

View full text Add to dashboard Cite

“…However, there are several incongruous and moderately significant results demonstrating that the higher the disease stage, the higher the yield of detected CTCs. Although statistically not significant, a trend regarding this conclusion was shown by Buglione et al [82] last year, comparing T1 to higher tumour stages in HN-SCC, but in the OSCC subgroup (13/152 patients), no CTCs were found. Previously, Guney et al [83] found similar results for HNSCC but in a smaller cohort of patients.…”

Section: A Discussion Of the Results From Studies On Head And Neck Camentioning

confidence: 77%

“…Regarding the therapeutic response in HN-SCC with decreased levels of previously detected CTCs, a positive effect on outcome was presumed by Buglione et al [82] because partial or complete response could be stated along with these findings.…”

Section: A Discussion Of the Results From Studies On Head And Neck Camentioning

confidence: 96%

Squamous cell carcinoma of the oral cavity and circulating tumour cells

Wikner

Gröbe

Pantel

2014

WJCO

View full text Add to dashboard Cite

Due to a lack of substantial improvement in the outcome of patients suffering from oral squamous cell carcinoma (OSCC) during the past decades, current staging methods need to be revised. This disease is associated with poor survival rates despite considerable advances in diagnosis and treatment. The early detection of metastases is an important indicator of survival, prognosis and relapse. Therefore, a better understanding of the mechanisms underlying metastasis is crucial. Exploring alternative measures apart from common procedures is needed to identify new prognostic markers. Similar to previous findings predominantly for other solid tumours, recently published studies demonstrate that circulating tumour cells (CTCs) and disseminated tumour cells (DTCs) might serve as prognostic markers and could supplement routine staging in OSCC. Thus, the detection of CTCs/DTCs is a promising tool to determine the individual need for therapeutic intervention. Encouraging results and new approaches point to the future use of targeted therapies for OSCC, an exceedingly heterogeneous subgroup of head and neck cancer. This review focuses on summarising technologies currently used to detect CTCs/DTCs. The translational relevance for OSCC is highlighted. The inherent challenges in detecting CTCs/DTCs will be emphasised. Core tip: Oral squamous cell carcinoma (OSCC), among head and neck cancer, is related to poor survival rates despite considerable advances in diagnosis and treatment. Therefore, detecting tumour cell dissemination early and understanding the underlying mechanisms are crucial for predicting prognosis, relapse and survival. According to previous findings, circulating tumour cells (CTCs) and disseminated tumour cells (DTCs) might serve as prognostic markers to supplement routine staging and support determining individual therapeutic interventions. This review focuses on summarising the current knowledge about the detection of CTCs/DTCs with special emphasis on patients suffering from OSCC. The translational relevance of CTCs/DTCs and challenges for clinical application are highlighted.Wikner J, Gröbe A, Pantel K, Riethdorf S. Squamous cell carcinoma of the oral cavity and circulating tumour cells.

show abstract

Circulating Tumour Cells in locally advanced head and neck cancer: Preliminary report about their possible role in predicting response to non-surgical treatment and survival

Cited by 87 publications

References 29 publications

Prognostic Relevance of Circulating Tumor Cells in Blood and Disseminated Tumor Cells in Bone Marrow of Patients with Squamous Cell Carcinoma of the Oral Cavity

Prognostic Relevance of Circulating Tumor Cells in Blood and Disseminated Tumor Cells in Bone Marrow of Patients with Squamous Cell Carcinoma of the Oral Cavity

Targeting the PI3K/AKT/mTOR pathway in squamous cell carcinoma of the head and neck

Squamous cell carcinoma of the oral cavity and circulating tumour cells

Contact Info

Product

Resources

About