Circulating tumour cells and DNA as liquid biopsies in gastrointestinal cancer

Circulating tumor DNA quantity is related to tumor volume and both predict survival in metastatic pancreatic ductal adenocarcinoma

“…At baseline, there was no statistically significant relationship between CA19.9 and ctDNA ( n = 47; p = 0.170). Other studies have shown a correlation between CA19.9 and ctDNA, although the cohorts were of limited size . Surprisingly, we did not find ECOG status to be a significant prognosticator in the regression analysis.…”

“…Other studies have shown a correlation between CA19.9 and ctDNA, although the cohorts were of limited size. 25,36 Surprisingly, we did not find ECOG status to be a significant prognosticator in the regression analysis. This seems to be due to the fact that only ECOG performance status 3 had prognostic strength, but that there were only three patients with ECOG 3.…”

Effects of Lidocaine and Ropivacaine on Gastric Cancer Cells Through Down-regulation of ERK1/2 Phosphorylation In Vitro

“…After surgery, the recurrence and metastasis of gastric cancer are pressing issues, and are complicated processes involved a series of events (6,7), such as tumor heterogeneity, and epigenetic changes (8,9). It has been confirmed that circulating tumour cells with the ability to diffuse and metastasize are a prime prognostic marker for cancer recurrence (10,11). Even if patients undergo a complete resection of their tumour, circulating tumour cells shed from the tumour into the circulatory or lymphatic system, enabling the recurrence and metastasis of cancer (12).…”

Liquid biopsy in pancreatic ductal adenocarcinoma: current status of circulating tumor cells and circulating tumor DNA

“…ctDNA is a subset of circulating extracellular DNA in plasma (also called cell‐free DNA, cfDNA), specifically released from cancer cells. ctDNA (known as tumor‐derived cfDNA) may originate from apoptotic and necrotic tumor cells, from living tumor cells, or even from CTCs; thus, it has a variable half‐life from 15 minutes up to 2 h (Alix‐Panabieres and Pantel, ; Diaz and Bardelli, ; Diehl et al ., ; Kidess and Jeffrey, ; Nordgard et al ., ). While the size of cfDNA released by apoptotic cells represents approximately 166 bp, ctDNA has recently been reported as being more highly fragmented (Mouliere and Rosenfeld, ; Underhill et al ., ).…”